Establishment of novel HCV cell-culture system using HCV strains other than JFH-1
| Project/Area Number |
23790513
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
MURAYAMA Asako 国立感染症研究所, ウイルス第二部, 協力研究員 (40415534)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ウイルス / 細胞培養 / ゲノム複製 / C型肝炎ウイルス |
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| Research Abstract |
We established a novel HCV genotype 2a cell-culture system using J6CF strain harboring five mutations in NS4A, NS5B and 3'UTR. We also established an HCV genotype 2b cell-culture system using a chimeric genome of MA strain harboring minimal regions of JFH-1. These cell-culture systems will be useful for characterizing genotype 2a and 2b viruses and developing antiviral strategies. Our data provides information that will prove essential for the establishment of replication-competent variants of HCV strains that are currently replication incompetent in cultured cells.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Involvement of Hepatitis C Virus NS5A Hyperphosphorylation Mediated by Casein Kinase I-α in Infectious Virus Production2014
Author(s)
Masaki T, Matsunaga S, Takahashi H, Nakashima K, Kimura Y, Ito M, Matsuda M, Murayama A,Kato T, Hirano H, Endo Y, Lemon SM, Wakita T, Sawasaki T, Suzuki T
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Journal Title
Journal of Virology
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Novel cell culture-adapted genotype 2a hepatitis C virus infectious clone2012
Author(s)
Date T, Kato T, Kato J, Takahashi H, Morikawa K, Akazawa D, Murayama A, Tanaka-Kaneko K, Sata T, Tanaka Y, Mizokami M, Wakita T
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Journal Title
J Virol
Volume: 86(19)
Pages: 10805-10820
Related Report
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[Journal Article] Production of Infectious Chimeric Hepatitis C Virus Genotype 2b Harboring Minimal Regions of JFH-12012
Author(s)
Murayama A, Kato T, Akazawa D, Sugiyama N, Date T, Masaki T, Nakamoto S, Tanaka Y, Mizokami M, Yokosuka O, Nomoto A, Wakita T
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Journal Title
J Virol
Volume: 86(4)
Pages: 2143-2152
Related Report
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[Journal Article] In vivo adaptation of hepatitis C virus in chimpanzees for efficient virus production and evasion of apoptosis2011
Author(s)
Saeed M, Shiina M, Date T, Akazawa D, Watanabe N, Murayama A, Suzuki T, Watanabe H, Hiraga N, Imamura M, Chayama K, Choi Y, Krawczynski K, Liang TJ, Wakita T, Kato T
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Journal Title
Hepatology
Volume: 54
Issue: 2
Pages: 425-433
DOI
Related Report
Peer Reviewed
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