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Mechanistic analysis of hepatitis C virus life cycle using small molecules

Research Project

Project/Area Number 23790514
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Virology
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

WATASHI Koichi  国立感染症研究所, ウイルス第二部, 主任研究官 (40378948)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsHCV / PLD / 抗ウイルス / ウイルス / phospholipase / 肝炎
Research Abstract

We analyzed the anti-hepatitis C virus (HCV) mechanism of halopemide, which was identified to reduce HCV production by a cell-based chemical screening. Halopemide inhibited the secretion process of HCV. Its anti-HCV effect was mediated by host phospholipase D (PLD). PLD regulated the release of HCV but not host secreted proteins. Inhibition of PLD stalled HCV particles around the Golgi. The above data suggest that host PLD specifically mediates the post-Golgi trafficking of HCV, and halopemide inhibited this process to reduce the production of infectious HCV.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (107 results)

All 2014 2013 2012 2011 Other

All Journal Article (22 results) (of which Peer Reviewed: 6 results) Presentation (79 results) (of which Invited: 3 results) Book (4 results) Remarks (2 results)

  • [Journal Article] Cyclosporin A and its analogs inhibit hepatitis B virus entry into cultured hepatocytes through targeting a membrane transporter NTCP2014

    • Author(s)
      Watashi K, Sluder A, Daito T, Matsunaga S, Ryo A, Nagamori S, Iwamoto M, Nakajima S, Tsukuda S, Borroto-Esoda K, Sugiyama M, Tanaka Y, Kanai Y, Kusuhara H, Mizokami M, Wakita T.
    • Journal Title

      Hepatology

      Volume: 59 Pages: 1726-1737

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] NTCP and beyond : opening the door to unveil hepatitis B virus entry2014

    • Author(s)
      Watashi K, Urban S, Li W, Wakita T.
    • Journal Title

      Int J Mol Sci

      Volume: 15 Pages: 2892-2905

    • Related Report
      2013 Final Research Report
  • [Journal Article] NTCP transporter as novel target for anti-hepatitis B virus agents2014

    • Author(s)
      Tsukuda S, Watashi K.
    • Journal Title

      Clin Res Infect Dis

      Volume: 1

    • Related Report
      2013 Final Research Report
  • [Journal Article] Evaluation and identification of hepatitis B virus entry inhibitors using HepG2 cells overexpressing a membrane transporter NTCP2014

    • Author(s)
      Iwamoto M, Watashi K, Tsukuda S, Aly HH, Fukasawa M, Fujimoto A, Suzuki R, Aizaki H, Ito T, Koiwai O, Kusuhara H, Wakita T.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 443 Pages: 808-813

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Production of single-round infectious chimeric flaviviruses with DNA-based Japanese encephalitis virus replicon2014

    • Author(s)
      Suzuki R, Ishikawa T, Konishi E, Matsuda M, Watashi K, Aizaki H, Takasaki T, Wakita T
    • Journal Title

      J Gen Virol

      Volume: 95 Pt 1 Pages: 60-65

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] NTCP and beyond: opening the door to unveil hepatitis B virus entry2014

    • Author(s)
      Watashi K, Urban S, Li W, Wakita T
    • Journal Title

      Int J Mol Sci

      Volume: 15 Issue: 2 Pages: 2892-2905

    • DOI

      10.3390/ijms15022892

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Total Synthesis and Anti-Hepatitis C Virus Activity of MA0262013

    • Author(s)
      Shimura S, Ishima M, Nakajima S, Fujii T, Himeno N, Ikeda K, Izaguirre-Carbonell J, Murata H, Takeuchi T, Kamisuki S, Suzuki T, Kuramochi K, Watashi K, Kobayashi S, Sugawara F
    • Journal Title

      J Am Chem Soc

      Volume: 135 Pages: 18949-18956

    • Related Report
      2013 Final Research Report
  • [Journal Article] Specific inhibition of hepatitis C virus entry into host hepatocytes by fungi-derived sulochrin and its derivatives2013

    • Author(s)
      Nakajima S, Watashi K, Kamisuki S, Tsukuda S, Takemoto K, Matsuda M, Suzuki R, Aizaki H, Sugawara F, Wakita T.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 440 Pages: 515-520

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Interleukin-1 and tumor necrosis factor-αtrigger restriction of hepatitis B virus infection via a cytidine deaminase activation-induced cytidine deaminase (AID)2013

    • Author(s)
      Watashi K, Liang G, Iwamoto M, Marusawa H, Uchida N, Daito T, Kitamura K, Muramatsu M, Ohashi H, Kiyohara T, Suzuki R, Li J, Tong S, Tanaka Y, Murata K, Aizaki H, Wakita T.
    • Journal Title

      J Biol Chem

      Volume: 288 Pages: 31715-31727

    • NAID

      120006602140

    • Related Report
      2013 Final Research Report
  • [Journal Article] Signal peptidase complex subunit 1 participates in the assembly of hepatitis C virus through an interaction with E2 and NS22013

    • Author(s)
      Suzuki R, Matsuda M, Watashi K, Aizaki H, Matsuura Y, Wakita T, Suzuki T
    • Journal Title

      PLoS Pathog

      Volume: 9

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Antiviral activity of glycyrrhizin against hepatitis C virus in vitro2013

    • Author(s)
      Matsumoto Y, Matsuura T, Aoyagi H, Matsuda M, Hmwe SS, Date T, Watanabe N, Watashi K, Suzuki R, Ichinose S, Wake K, Suzuki T, Miyamura T, Wakita T, Aizaki H
    • Journal Title

      PLoS One

      Volume: 8

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] 薬学的視点からのウイルス学研究-肝炎ウイルス複製阻害化合物の同定とその作用機序-2013

    • Author(s)
      渡士幸一
    • Journal Title

      薬学雑誌

      Volume: 133 Pages: 1169-1175

    • NAID

      130003382269

    • Related Report
      2013 Final Research Report
  • [Journal Article] Total synthesis and anti-hepatitis C virus activity of MA0262013

    • Author(s)
      Shimura S, Ishima M, Nakajima S, Fujii T, Himeno N, Ikeda K, Izaguirre-Carbonell J, Murata H, Takeuchi T, Kamisuki S, Suzuki T, Kuramochi K, Watashi K, Kobayashi S, Sugawara F
    • Journal Title

      J Am Chem Soc

      Volume: 135 Issue: 50 Pages: 18949-18956

    • DOI

      10.1021/ja410145x

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interleukin-1 and tumor necrosis factor-α trigger restriction of hepatitis B virus infection via a cytidine deaminase activation-induced cytidine deaminase (AID)2013

    • Author(s)
      Watashi K, Liang G, Iwamoto M, Marusawa H, Uchida N, Daito T, Kitamura K, Muramatsu M, Ohashi H, Kiyohara T, Suzuki R, Li J, Tong S, Tanaka Y, Murata K, Aizaki H, Wakita T
    • Journal Title

      J Biol Chem

      Volume: 288 Issue: 44 Pages: 31715-31727

    • DOI

      10.1074/jbc.m113.501122

    • NAID

      120006602140

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 薬学的視点からのウイルス学研究―肝炎ウイルス複製阻害化合物の同定とその作用機序2013

    • Author(s)
      渡士幸一
    • Journal Title

      薬学雑誌

      Volume: 133 Pages: 1169-1175

    • NAID

      130003382269

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Different mechanisms of hepatitis C virus RNA polymerase activation by cyclophilin A and B in vitro2012

    • Author(s)
      Weng L, Tian X, Gao Y, Watashi K, Shimotohno K, Wakita T, Kohara M, Toyoda T
    • Journal Title

      Biochim Biophys Acta

      Volume: 1820 Pages: 1886-1892

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Japanese reference panel of blood specimens for evaluation of hepatitis C virus RNA and core antigen quantitative assays2012

    • Author(s)
      Murayama A, Sugiyama N, Watashi K, Masaki T, Suzuki R, Aizaki H, Mizuochi T, Wakita T, Kato T
    • Journal Title

      J Clin Microbiol

      Volume: 50 Pages: 1943-1949

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] HBV培養細胞系による新規抗ウイルス化合物のスクリーニング2012

    • Author(s)
      渡士幸一
    • Journal Title

      肝胆膵

      Volume: 65 Pages: 611-617

    • Related Report
      2013 Final Research Report
  • [Journal Article] Japanese reference panel of blood specimens for evaluation of hepatitis C virus RNA and core antigen quantitative assays.2012

    • Author(s)
      Maruyama, A.
    • Journal Title

      J. Clin. Microbiol.

      Volume: 50(6) Issue: 6 Pages: 1943-1949

    • DOI

      10.1128/jcm.00487-12

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Potent and selective inhibition of hepatitis C virus replication by novel phenanthridinone derivatives2011

    • Author(s)
      Salim MT, Aoyama H, Sugita K, Watashi K, Wakita T, Hamasaki T, Okamoto M, Urata Y, Hashimoto Y, Baba M
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 415 Pages: 714-719

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Cyclosporin A associated helicase-like protein facilitates the association of hepatitis C virus RNA polymerase with its cellular cyclophilin B2011

    • Author(s)
      Morohashi K+, Sahara H, Watashi K+, Iwabata K, Sunoki T, Kuramochi K, Takakusagi K, Miyashita H, Sato N, Tanabe A, Shimotohno K, Kobayashi S, Sakaguchi K, Sugawara F. (+equally contributed)
    • Journal Title

      PLoS One

      Volume: 6

    • Related Report
      2013 Final Research Report
  • [Journal Article] Cyclosporin A Associated Helicase-Like Protein Facilitates the Association of Hepatitis C Virus RNA Polymerase with Its Cellular Cyclophilin B2011

    • Author(s)
      諸橋謙吾、佐原広益、小林進、坂口謙吾、菅原二三男
    • Journal Title

      PLoS ONE

      Volume: 6 Issue: 4 Pages: 18285-18285

    • DOI

      10.1371/journal.pone.0018285

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] B型肝炎ウイルス感染を抑制するサイトカインの同定とその分子メカニズムの解析2014

    • Author(s)
      渡士幸一
    • Organizer
      Liver Forum in Kyoto
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] IL-1- and TNFalpha-triggered intracellular immune response against HBV2013

    • Author(s)
      K. Watashi
    • Organizer
      1^<st> Japan-Taiwan Research Symposium on Hepatitis B virus
    • Place of Presentation
      Tokyo(Japan)
    • Related Report
      2013 Final Research Report
  • [Presentation] Cyclophilin inhibitors potentiate interferon signaling through diminished PKR phosphorylation in HCV-infected cells2013

    • Author(s)
      K. Watashi, T. Daito, A. Sluder, K. Borroto-Esoda, T. Wakita
    • Organizer
      European Association for the Study of the Liver 2013
    • Place of Presentation
      Amsterdam(Netherlands)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] ウイルス感染系を基盤とした真菌由来天然化合物の生理活性探索2013

    • Author(s)
      渡士幸一、中嶋翔
    • Organizer
      天然物ケミカルバイオロジー第4回公開シンポジウム
    • Place of Presentation
      つくば
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Novel regulation mechanism of interferon signaling by cyclophilin through modulation of PKR in HCV-infected cells2013

    • Author(s)
      Watashi, T. Daito, A. Sluder, K. Borroto-Esoda, T. Wakita
    • Organizer
      20th International Symposium on Hepatitis C Virus and Related Viruses
    • Place of Presentation
      Melbourne(Australia)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Isolation of a natural product inhibiting the transcriptional activity of liver X receptor and reducing the production of infectious HCV2013

    • Author(s)
      S. Nakajima, K. Watashi, S. Kamisuki, K. Takemoto, R. Suzuki, H. Aizaki, F. Sugawara, T. Wakita
    • Organizer
      20th International Symposium on Hepatitis C Virus and Related Viruses
    • Place of Presentation
      Melbourne(Australia)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Dynamics of the cellular metabolome during hepatitis C virus infection–Regulation of the lipoprotein metabolisms by hepatic lipase -2013

    • Author(s)
      A. Fujimoto, H. Aizaki, M. Matsuda, N. Watanabe, K. Watashi, R. Suzuki, T. Suzuki, T. Miyamura, T. Wakita
    • Organizer
      20th International Symposium on Hepatitis C Virus and Related Viruses
    • Place of Presentation
      Melbourne(Australia)
    • Related Report
      2013 Final Research Report
  • [Presentation] Cyclosporin A and its analogs inhibit hepatitis B virus entry into cultured hepatocytes through targeting sodium taurocholate cotransporting polypeptide2013

    • Author(s)
      K. Watashi, A. Sluder, S. Matsunaga, Ryo, S. Nakajima, M. Iwamoto, S. Tsukuda, K. Borroto-Esoda, M. Sugiyama, Y. Tanaka, M. Mizokami, T. Wakita
    • Organizer
      2013 International Meeting on Molecular Biology of Hepatitis B viruses
    • Place of Presentation
      Shanghai(China)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Interleukin-1 and tumor necrosis factor-alpha trigger restriction of hepatitis B virus infection via a cytidine deaminase AID2013

    • Author(s)
      K. Watashi, G. Liang, M. Iwamoto, H. Marusawa, K. Kitamura, M. Muramatsu, R. Suzuki, J. Li, S. Tong, Y. Tanaka, K. Murata, H. Aizaki, T. Wakita
    • Organizer
      2013 International Meeting on Molecular Biology of Hepatitis B viruses
    • Place of Presentation
      Shanghai(China)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Mechanistic analysis on hepatitis B virus entry in an NTCP-overexpressing cell line2013

    • Author(s)
      M. Iwamoto, K. Watashi, S. Tsukuda, H. Aly, R. Suzuki, H. Aizaki, O. Koiwai, H. Kusuhara, T. Wakita
    • Organizer
      2013 International Meeting on Molecular Biology of Hepatitis B viruses
    • Place of Presentation
      Shanghai(China)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] A retinoid derivative inhibits hepatitis B virus entry mediated by NTCP2013

    • Author(s)
      S. Tsukuda, K. Watashi, M. Iwamoto, R. Suzuki, H. Aizaki, S. Kojima, T. Wakita
    • Organizer
      2013 International Meeting on Molecular Biology of Hepatitis B viruses
    • Place of Presentation
      Shanghai(China)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] The discovery of a new virus/host interaction regulating HBV life cycle2013

    • Author(s)
      H. Aly, K. Watashi, K. Chayama, T. Wakita
    • Organizer
      2013 International Meeting on Molecular Biology of Hepatitis B viruses
    • Place of Presentation
      Shanghai(China)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Formation of covalently closed circular (ccc)DNA tetracycline inducible HBV expression cell line2013

    • Author(s)
      N. Ogura, K. Watashi, T. Wakita
    • Organizer
      2013 International Meeting on Molecular Biology of Hepatitis B viruses
    • Place of Presentation
      Shanghai(China)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Analysis of bioactivity of fungal-derived natural products based on a virus infection system2013

    • Author(s)
      S. Nakajima, K. Watashi
    • Organizer
      The 2nd International Symposium on Chemical Biology of Natural Products
    • Place of Presentation
      Yokohama(Japan)
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] シチジンデアミナーゼAID誘導を介した抗B型肝炎ウイルス細胞内免疫応答機構の解明2013

    • Author(s)
      渡士幸一、Guoxin Liang、岩本将士、丸澤宏之、喜多村晃一、村松正道、鈴木亮介、相崎英樹、脇田隆字
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] PKR活性化制御を介するシクロフィリン阻害剤の新たな抗C型肝炎ウイルス作用機序2013

    • Author(s)
      大東卓史、渡士幸一、Ann Sluder、中嶋翔、Katyna Borroto-Esoda、藤田尚志、脇田隆字
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] C型肝炎ウイルス分泌過程はphospholipase Dが関わる膜輸送により制御される2013

    • Author(s)
      内田奈々子、渡士幸一、中嶋翔、岩本将士、鈴木亮介、相崎英樹、千葉丈、脇田隆字
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] ヒトNTCP安定発現細胞株におけるB型肝炎ウイルス侵入機構の解析2013

    • Author(s)
      岩本将士、渡士幸一、九十田千子、アリフセイン、鈴木亮介、相崎英樹、小祝修、楠原洋之、脇田隆字
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] B型肝炎ウイルス侵入阻害剤の同定およびそのNTCPを介した感染阻害機構の解明2013

    • Author(s)
      九十田千子、渡士幸一、岩本将士、鈴木亮介、相崎英樹、小嶋聡一、脇田隆字
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Liver X receptor転写活性および感染性C型肝炎ウイルス粒子産生を阻害する天然化合物の同定2013

    • Author(s)
      中嶋翔、渡士幸一、紙透伸治、竹本健二、鈴木亮介、相崎英樹、菅原二三男、脇田隆字
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Study of new virus/host interactions regulating HBV life cycle2013

    • Author(s)
      フセインアリ、渡士幸一、茶山一彰、脇田隆字
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 日本脳炎ウイルスレプリコンを用いたトランスパッケージング型1回感染性フラビウイルス粒子産生系の開発2013

    • Author(s)
      鈴木亮介、石川知弘、小西英二、嵯峨涼平、松田麻未、渡士幸一、相崎英樹、高崎智彦、脇田隆字
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] C型肝炎ウイルス感染による宿主細胞の脂質代謝変化とHepatic Lipase発現制御2013

    • Author(s)
      藤本陽、相崎英樹、松田麻未、渡邉則幸、渡士幸一、鈴木亮介、鈴木哲朗、宮村達男、脇田隆字
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] C型肝炎ウイルスNS5A結合膜タンパク質ELAVL1のウイルス複製・翻訳スイッチング機構の解析2013

    • Author(s)
      後藤耕司、相崎英樹、渡邉則幸、渡士幸一、鈴木亮介、山越智、四柳宏、森屋恭爾、小池和彦、鈴木哲朗、宮村達男、脇田隆字
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 細胞内発現抗体(イントラボディ)によるC型肝炎ウイルスの増殖抑制2013

    • Author(s)
      松田麻未、斎藤憲司、鈴木亮介、佐藤充、鐘ヶ江裕美、渡士幸一、相崎英樹、千葉丈、斎藤泉、脇田隆字、鈴木哲朗
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] PhospholipaseA2およびAutophagyによるC型肝炎ウイルス(HCV)分泌過程の制御-グリチルリチンによる抗HCV作用-2013

    • Author(s)
      青柳東代、相崎英樹、松本喜弘、松田麻未、SuSu Hmwe、渡邉則幸、渡士幸一、鈴木亮介、市野瀬志津子、松浦知和、鈴木哲朗、和気健二郎、宮村達男、脇田隆字
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Tetherin/BST-2はHBV複製を負に制御する2013

    • Author(s)
      古賀れい奈、宮川敬、松永智子、渡士幸一、脇田隆字、梁明秀
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 新規ピラノンカルボキサミド誘導体の抗HCV効果とその構造活性相関2013

    • Author(s)
      馬場昌範、外山政明、伊藤渉、岡本実佳、渡士幸一、脇田隆字、Sharon Ashoke
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] プラスミドトランスフェクションによるトランスパッケージング型1回感染性フラビウイルス産生系の確立2013

    • Author(s)
      鈴木亮介、石川知弘、小西英二、嵯峨涼平、松田麻未、渡士幸一、相崎英樹、高崎智彦、脇田隆字
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] PhospholipaseA2およびAutophagyによるC型肝炎ウイルス(HCV)分泌過程の制御-グリチルリチンによる抗HCV作用-2013

    • Author(s)
      青柳東代、相崎英樹、松本喜弘、松田麻未、SuSu Hmwe、渡邉則幸、渡士幸一、鈴木亮介、市野瀬志津子、松浦知和、鈴木哲朗、和気健二郎、宮村達男、脇田隆字
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 肝炎ウイルス複製を阻害する低分子化合物/生理活性物質の同定とその作用機序2013

    • Author(s)
      渡士幸一
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] IL-1- and TNFalpha-triggered intracellular immune response against HBV2013

    • Author(s)
      K. Watashi
    • Organizer
      1st Japan-Taiwan Research Symposium on Hepatitis B virus
    • Place of Presentation
      Tokyo(Japan)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Dynamics of the cellular metabolome during hepatitis C virus infection – Regulation of the lipoprotein metabolisms by hepatic lipase -2013

    • Author(s)
      A. Fujimoto, H. Aizaki, M. Matsuda, N. Watanabe, K. Watashi, R. Suzuki, T. Suzuki, T. Miyamura, T. Wakita
    • Organizer
      20th International Symposium on Hepatitis C Virus and Related Viruses
    • Place of Presentation
      Melbourne(Australia)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞内発現抗体(イントラボディ)によるC型肝炎ウイルスの増殖抑制2013

    • Author(s)
      松田麻未、斎藤憲司、鈴木亮介、佐藤充、鐘ヶ江裕美、渡士幸一、相崎英樹、千葉丈、斎藤泉、脇田隆字、鈴木哲朗
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] 数理モデルによる抗ウイルス薬の薬効評価系の確立2013

    • Author(s)
      小泉吉輝、岩見真吾、内田奈々子、脇田隆字、渡士幸一
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] 肝炎ウイルス複製を阻害する低分子化合物/生理活性物質の同定とその作用機序2013

    • Author(s)
      渡士幸一
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 低分子化合物を利用した肝炎ウイルス学解析2012

    • Author(s)
      渡士幸一
    • Organizer
      東京大学医科学研究所第一回感染症国際研究センターシンポジウム
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] 抗HBV剤探索とHBV感染増殖機構の解析2012

    • Author(s)
      渡士幸一
    • Organizer
      LIVER2012第8回肝免疫・ウイルス・フロンティア肝疾患研究の新潮流
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthetic studies of MA026, a novel antiviral lipocyclodepsipeptide2012

    • Author(s)
      S. Shimura, M. Ishima, I. Ota, E. Tsutsui, S. kamisuki, H. Murata, T. Yamazaki, T. Suzuki, K. Kuramochi, T. Takeuchi, K. Watashi, S. Kobayashi, F. Sugawara
    • Organizer
      International Congress on Natural Products Research 2012
    • Place of Presentation
      New York(USA)
    • Related Report
      2013 Final Research Report
  • [Presentation] 数理モデルを用いた肝炎ウイルスの解析2012

    • Author(s)
      渡士幸一
    • Organizer
      第22回数理生物学会
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Interleukin-1 and tumor necrosis factor-alpha suppressed hepatitis B virus infection through NF-kappaB signaling pathway2012

    • Author(s)
      K. Watashi, N. Uchida, T. Daito, T. Kiyohara, R. Suzuki, H. Aizaki, T. Wakita
    • Organizer
      2012 International Meeting on Molecular Biology of Hepatitis B viruses
    • Place of Presentation
      Oxford(England)
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of cell clones for analyzing anti-hepatitis B virus compounds2012

    • Author(s)
      K. Watashi, M. Iwamoto, N. Uchida, T. Kiyohara, R. Suzuki, H. Aizaki, T. Wakita
    • Organizer
      2012 International Meeting on Molecular Biology of Hepatitis B viruses
    • Place of Presentation
      Oxford(England)
    • Related Report
      2013 Final Research Report
  • [Presentation] Characterization of anti-HCV release inhibitors targeting phospholipase D2012

    • Author(s)
      K. Watashi, N. Uchida, M. Saeed, R. Suzuki, H. Aizaki, T. Wakita
    • Organizer
      19th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Venice(Italy)
    • Related Report
      2013 Final Research Report
  • [Presentation] Phospholipase D regulates membrane trafficking during hepatitis C virus egress2012

    • Author(s)
      N. Uchida, K. Watashi, R. Suzuki, H. Aizaki, J. Chiba, T. Wakita
    • Organizer
      19th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Venice(Italy)
    • Related Report
      2013 Final Research Report
  • [Presentation] Antiviral activity of glycyrrhizin against hepatitis C virus in vitro2012

    • Author(s)
      Y. Matsumoto, N. Watanabe, K. Watashi, R. Suzuki, T. Matsuura, T. Suzuki, T. Miyamura, K. Wake, T. Wakita, H. Aizaki
    • Organizer
      19th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Venice(Italy)
    • Related Report
      2013 Final Research Report
  • [Presentation] An alternative endocytosis pathway for the protective entry of hepatitis C virus2012

    • Author(s)
      R. Suzuki, M. Matsuda, K. Watashi, H. Aizaki, Y. Matsuura, T. Suzuki, T. Wakita
    • Organizer
      19th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Venice(Italy)
    • Related Report
      2013 Final Research Report
  • [Presentation] Construction of hepatitis C virus genotype 4a clone2012

    • Author(s)
      H.H. Aly, K. Watashi, N. Watanabe, M. Mizokami, T. Kato, T. Wakita
    • Organizer
      19th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Venice(Italy)
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of natural products inhibiting hepatitis C virus infection2012

    • Author(s)
      S. Nakajima, K. Watashi
    • Organizer
      The 1st International Symposium on Chemical Biology of Natural Products
    • Place of Presentation
      Kyoto(Japan)
    • Related Report
      2013 Final Research Report
  • [Presentation] IL-1およびTNF-alphaのB型肝炎ウイルス感染阻害効果2012

    • Author(s)
      渡士幸一、内田奈々子、大東卓史、清原知子、鈴木亮介、相崎英樹、脇田隆字
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Phospholipase D is a cellular regulator during hepatitis C virus egress and a possible target for antiviral strategy2012

    • Author(s)
      K. Watashi, N. Uchida, R. Suzuki, H. Aizaki, T. Wakita
    • Organizer
      Japan Society of Hepatology 10th Single Topic Conference
    • Place of Presentation
      Tokyo(Japan)
    • Related Report
      2013 Final Research Report
  • [Presentation] 抗HBV剤探索とHBV感染増殖機構の解析2012

    • Author(s)
      渡士幸一
    • Organizer
      LIVER2012 第8回肝免疫・ウイルス・フロンティア 肝疾患研究の新潮流
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Synthetic studies of MA026, a novel antiviral lipocyclodepsipeptide2012

    • Author(s)
      S. Shimura, M. Ishima, I. Ota, E. Tsutsui, S. kamisuki, H. Murata, T. Yamazaki, T. Suzuki, K. Kuramochi, T. Takeuchi, K. Watashi, S. Kobayashi, F. Sugawara
    • Organizer
      International Congress on Natural Products Research 2012
    • Place of Presentation
      New York (USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] Interleukin-1 and tumor necrosis factor-alpha suppressed hepatitis B virus infection through NF-kappaB signaling pathway2012

    • Author(s)
      K. Watashi, N. Uchida, T. Daito, T. Kiyohara, R. Suzuki, H. Aizaki, T. Wakita
    • Organizer
      2012 International Meeting on Molecular Biology of Hepatitis B viruses
    • Place of Presentation
      Oxford (England)
    • Related Report
      2012 Research-status Report
  • [Presentation] Development of cell clones for analyzing anti-hepatitis B virus compounds2012

    • Author(s)
      K. Watashi, M. Iwamoto, N. Uchida, T. Kiyohara, R. Suzuki, H. Aizaki, T. Wakita
    • Organizer
      2012 International Meeting on Molecular Biology of Hepatitis B viruses
    • Place of Presentation
      Oxford (England)
    • Related Report
      2012 Research-status Report
  • [Presentation] Characterization of anti-HCV release inhibitors targeting phospholipase D2012

    • Author(s)
      K. Watashi, N. Uchida, M. Saeed, R. Suzuki, H. Aizaki, T. Wakita
    • Organizer
      19th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Venice (Italy)
    • Related Report
      2012 Research-status Report
  • [Presentation] Phospholipase D regulates membrane trafficking during hepatitis C virus egress2012

    • Author(s)
      N. Uchida, K. Watashi, R. Suzuki, H. Aizaki, J. Chiba, T. Wakita
    • Organizer
      19th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Venice (Italy)
    • Related Report
      2012 Research-status Report
  • [Presentation] Antiviral activity of glycyrrhizin against hepatitis C virus in vitro2012

    • Author(s)
      Y. Matsumoto, N. Watanabe, K. Watashi, R. Suzuki, T. Matsuura, T. Suzuki, T. Miyamura, K. Wake, T. Wakita, H. Aizaki
    • Organizer
      19th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Venice (Italy)
    • Related Report
      2012 Research-status Report
  • [Presentation] An alternative endocytosis pathway for the protective entry of hepatitis C virus2012

    • Author(s)
      R. Suzuki, M. Matsuda, K. Watashi, H. Aizaki, Y. Matsuura, T. Suzuki, T. Wakita
    • Organizer
      19th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Venice (Italy)
    • Related Report
      2012 Research-status Report
  • [Presentation] Construction of hepatitis C virus genotype 4a clone2012

    • Author(s)
      H.H. Aly, K. Watashi, N. Watanabe, M. Mizokami, T. Kato, T. Wakita
    • Organizer
      19th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Venice (Italy)
    • Related Report
      2012 Research-status Report
  • [Presentation] Identification of natural products inhibiting hepatitis C virus infection2012

    • Author(s)
      S. Nakajima, K. Watashi
    • Organizer
      The 1st International Symposium on Chemical Biology of Natural Products
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] Phospholipase D is a cellular regulator during hepatitis C virus egress and a possible target for antiviral strategy2012

    • Author(s)
      K. Watashi, N. Uchida, R. Suzuki, H. Aizaki, T. Wakita
    • Organizer
      Japan Society of Hepatology 10th Single Topic Conference
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Identification and functional analysis of small molecules inhibiting the late step of hepatitis C virus life cycle2011

    • Author(s)
      K. Watashi, N. Uchida, R. Suzuki, H. Aizaki, T. Wakita
    • Organizer
      18th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Seattle, USA
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] Identification of a novel cellular RNA helicase-like protein as a target for cyclosporin A that is involved in hepatitis C virus genome replication2011

    • Author(s)
      K. Watashi, H. Sahara, K. Morohashi, K. Iwabata, T. Sunoki, K. Kuramochi, K. Takakusagi, H. Miyashita, N. Sato, A. Tanabe, K. Shimotohno, S. Kobayashi, K. Sakaguchi, F. Sugawara
    • Organizer
      18th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Seattle, USA
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] Halopemide inhibited a post-assembly step in hepatitis C virus life cycle2011

    • Author(s)
      N. Uchida, K. Watashi, R. Suzuki, H. Aizaki, J. Chiba, T. Wakita
    • Organizer
      18th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Seattle, USA
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] Identification of lipid droplet-associated membrane proteins that are involved in HCV production2011

    • Author(s)
      H. Aizaki, Y. Matsumoto, K. Goto, K. Watashi, R. Suzuki, M. Fukasawa, K. Hanada, S. Sato, N. Takahashi, Y. Matsuura, K. Motojima, T. Miyamura, T. Suzuki, T. Wakita
    • Organizer
      18th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Seattle, USA
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] Identification of novel NS5A-associated proteins in the host-cell membrane fraction and their International Symposium on hepatitis C virus and related viruses2011

    • Author(s)
      K. Goto, T. Kimura, K. Watashi, R. Suzuki, S. Yamagoe, T. Miyamura, K. Moriya, H. Yotsuyanagi, K. Koike, T. Suzuki, T. Wakita, H. Aizaki
    • Organizer
      18th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Seattle, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Signal peptidase complex 1 participates in the assembly of hepatitis C virus through an interaction with NS22011

    • Author(s)
      R. Suzuki, T. Suzuki, K. Saito, M. Matsuda, K. Watashi, Y. Matsuura, T. Wakita, H. Aizaki
    • Organizer
      18th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Seattle, USA
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] Identification of small molecules affecting late steps of hepatitis C virus life cycle2011

    • Author(s)
      K. Watashi, N. Uchida, R. Suzuki, H. Aizaki, T. Wakita
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of a host factor that interacts with hepatitis C virus NS2 protein and is involved in the viral assembly2011

    • Author(s)
      R. Suzuki, T. Suzuki, K. Saito, M. Matsuda, K. Watashi, Y. Matsuura, T. Wakita, H. Aizaki
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
  • [Presentation] Antiviral activity of glycyrrhizin against hepatitis C virus in vitro2011

    • Author(s)
      Y. Matsumoto, K. Watashi, R. Suzuki, T. Matsuura, T. Suzuki, T. Miyamura, K. Wake, T. Wakita, H. Aizaki
    • Organizer
      International Union of Microbiological Societies 2011 Congres
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
  • [Presentation] Screening of small molecules affecting the production of hepatitis B virus2011

    • Author(s)
      K. Watashi, N. Uchida, R. Suzuki, H. Aizaki, T. Wakita
    • Organizer
      2011 International Meeting on Molecular Biology of Hepatitis B viruses
    • Place of Presentation
      Lake Buena Vista, USA
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 数理モデルと肝炎ウイルス解析2011

    • Author(s)
      渡士幸一
    • Organizer
      第8回生物数学の理論とその応用
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 低分子化合物を利用した肝炎ウイルス学解析2011

    • Author(s)
      渡士幸一
    • Organizer
      感染・免疫・炎症・発癌
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of novel NS5A-associated proteins in the host-cell membrane fraction and their role in HCV life cycle2011

    • Author(s)
      Goto K, Kimura T, Watashi K, Suzuki R, Yamagoe S, Miyamura T, Moriya K, Yotsuyanagi H, Koike K, Suzuki T, Wakita T, Aizaki H
    • Organizer
      18th International Symposium on hepatitis C virus and related viruses
    • Place of Presentation
      Seattle, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Identification of small molecules affecting late steps of hepatitis C virus life cycle2011

    • Author(s)
      Watashi K, Uchida N, Suzuki R, Aizaki H, Wakita T
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Identification of a host factor that interacts with hepatitis C virus NS2 protein and is involved in the viral assembly2011

    • Author(s)
      Suzuki R, Suzuki T, Saito K, Matsuda M, Watashi K, Matsuura Y, Wakita T, Aizaki H
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] Antiviral activity of glycyrrhizin against hepatitis C virus in vitro2011

    • Author(s)
      Matsumoto Y, Watashi K, Suzuki R, Matsuura T, Suzuki T, Miyamura T, Wake K, Wakita T, Aizaki H. Antiviral activity of glycyrrhizin against hepatitis C virus in vitro
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2011 Research-status Report
  • [Presentation] 数理モデルと肝炎ウイルス解析2011

    • Author(s)
      渡士幸一
    • Organizer
      第8回生物数学の理論とその応用(招待講演)
    • Place of Presentation
      京都
    • Related Report
      2011 Research-status Report
  • [Presentation] 低分子化合物を利用した肝炎ウイルス学解析2011

    • Author(s)
      渡士幸一
    • Organizer
      感染・免疫・炎症・発癌シンポジウム(招待講演)
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Book] 肝疾患Review2014-20152014

    • Author(s)
      渡士幸一
    • Publisher
      日本メディカルセンター
    • Related Report
      2013 Annual Research Report
  • [Book] エッセンシャルウイルス学2014

    • Author(s)
      渡士幸一
    • Publisher
      南江堂
    • Related Report
      2013 Annual Research Report
  • [Book] 肝疾患Review2014-2015

    • Author(s)
      渡士幸一
    • Publisher
      日本メディカルセンター(印刷中)
    • Related Report
      2013 Final Research Report
  • [Book] エッセンシャルウイルス学

    • Author(s)
      渡士幸一
    • Publisher
      南江堂(印刷中)
    • Related Report
      2013 Final Research Report
  • [Remarks] 渡士幸一、日本肝臓学会第10回シングルトピックカンファレンスYoung Investigator Award (2012)

    • Related Report
      2013 Final Research Report
  • [Remarks] 渡士幸一、肝炎ウイルス研究財団研究奨励(2012)

    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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