Mechanistic analysis of hepatitis C virus life cycle using small molecules

• Project/Area Number: 23790514
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: WATASHI Koichi 国立感染症研究所, ウイルス第二部, 主任研究官 (40378948)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: HCV / PLD / 抗ウイルス / ウイルス / phospholipase / 肝炎

Research Abstract

We analyzed the anti-hepatitis C virus (HCV) mechanism of halopemide, which was identified to reduce HCV production by a cell-based chemical screening. Halopemide inhibited the secretion process of HCV. Its anti-HCV effect was mediated by host phospholipase D (PLD). PLD regulated the release of HCV but not host secreted proteins. Inhibition of PLD stalled HCV particles around the Golgi. The above data suggest that host PLD specifically mediates the post-Golgi trafficking of HCV, and halopemide inhibited this process to reduce the production of infectious HCV.

Research Products

• [Journal Article] Cyclosporin A and its analogs inhibit hepatitis B virus entry into cultured hepatocytes through targeting a membrane transporter NTCP (2014)
  • Author(s): Watashi K, Sluder A, Daito T, Matsunaga S, Ryo A, Nagamori S, Iwamoto M, Nakajima S, Tsukuda S, Borroto-Esoda K, Sugiyama M, Tanaka Y, Kanai Y, Kusuhara H, Mizokami M, Wakita T.
  • Journal Title: Hepatology Volume: 59 Pages: 1726-1737
• [Journal Article] NTCP and beyond : opening the door to unveil hepatitis B virus entry (2014)
  • Author(s): Watashi K, Urban S, Li W, Wakita T.
  • Journal Title: Int J Mol Sci Volume: 15 Pages: 2892-2905
• [Journal Article] NTCP transporter as novel target for anti-hepatitis B virus agents (2014)
  • Author(s): Tsukuda S, Watashi K.
  • Journal Title: Clin Res Infect Dis Volume: 1
• [Journal Article] Evaluation and identification of hepatitis B virus entry inhibitors using HepG2 cells overexpressing a membrane transporter NTCP (2014)
  • Author(s): Iwamoto M, Watashi K, Tsukuda S, Aly HH, Fukasawa M, Fujimoto A, Suzuki R, Aizaki H, Ito T, Koiwai O, Kusuhara H, Wakita T.
  • Journal Title: Biochem Biophys Res Commun Volume: 443 Pages: 808-813
• [Journal Article] Production of single-round infectious chimeric flaviviruses with DNA-based Japanese encephalitis virus replicon (2014)
  • Author(s): Suzuki R, Ishikawa T, Konishi E, Matsuda M, Watashi K, Aizaki H, Takasaki T, Wakita T
  • Journal Title: J Gen Virol Volume: 95 Pt 1 Pages: 60-65
• [Journal Article] NTCP and beyond: opening the door to unveil hepatitis B virus entry (2014)
  • Author(s): Watashi K, Urban S, Li W, Wakita T
  • Journal Title: Int J Mol Sci Volume: 15 Issue: 2 Pages: 2892-2905
  • DOI: 10.3390/ijms15022892
  • Peer Reviewed
• [Journal Article] Total Synthesis and Anti-Hepatitis C Virus Activity of MA026 (2013)
  • Author(s): Shimura S, Ishima M, Nakajima S, Fujii T, Himeno N, Ikeda K, Izaguirre-Carbonell J, Murata H, Takeuchi T, Kamisuki S, Suzuki T, Kuramochi K, Watashi K, Kobayashi S, Sugawara F
  • Journal Title: J Am Chem Soc Volume: 135 Pages: 18949-18956
• [Journal Article] Specific inhibition of hepatitis C virus entry into host hepatocytes by fungi-derived sulochrin and its derivatives (2013)
  • Author(s): Nakajima S, Watashi K, Kamisuki S, Tsukuda S, Takemoto K, Matsuda M, Suzuki R, Aizaki H, Sugawara F, Wakita T.
  • Journal Title: Biochem Biophys Res Commun Volume: 440 Pages: 515-520
• [Journal Article] Interleukin-1 and tumor necrosis factor-αtrigger restriction of hepatitis B virus infection via a cytidine deaminase activation-induced cytidine deaminase (AID) (2013)
  • Author(s): Watashi K, Liang G, Iwamoto M, Marusawa H, Uchida N, Daito T, Kitamura K, Muramatsu M, Ohashi H, Kiyohara T, Suzuki R, Li J, Tong S, Tanaka Y, Murata K, Aizaki H, Wakita T.
  • Journal Title: J Biol Chem Volume: 288 Pages: 31715-31727
  • NAID: 120006602140
• [Journal Article] Signal peptidase complex subunit 1 participates in the assembly of hepatitis C virus through an interaction with E2 and NS2 (2013)
  • Author(s): Suzuki R, Matsuda M, Watashi K, Aizaki H, Matsuura Y, Wakita T, Suzuki T
  • Journal Title: PLoS Pathog Volume: 9
• [Journal Article] Antiviral activity of glycyrrhizin against hepatitis C virus in vitro (2013)
  • Author(s): Matsumoto Y, Matsuura T, Aoyagi H, Matsuda M, Hmwe SS, Date T, Watanabe N, Watashi K, Suzuki R, Ichinose S, Wake K, Suzuki T, Miyamura T, Wakita T, Aizaki H
  • Journal Title: PLoS One Volume: 8
• [Journal Article] 薬学的視点からのウイルス学研究-肝炎ウイルス複製阻害化合物の同定とその作用機序- (2013)
  • Author(s): 渡士幸一
  • Journal Title: 薬学雑誌 Volume: 133 Pages: 1169-1175
  • NAID: 130003382269
• [Journal Article] Total synthesis and anti-hepatitis C virus activity of MA026 (2013)
  • Author(s): Shimura S, Ishima M, Nakajima S, Fujii T, Himeno N, Ikeda K, Izaguirre-Carbonell J, Murata H, Takeuchi T, Kamisuki S, Suzuki T, Kuramochi K, Watashi K, Kobayashi S, Sugawara F
  • Journal Title: J Am Chem Soc Volume: 135 Issue: 50 Pages: 18949-18956
  • DOI: 10.1021/ja410145x
  • Peer Reviewed
• [Journal Article] Interleukin-1 and tumor necrosis factor-α trigger restriction of hepatitis B virus infection via a cytidine deaminase activation-induced cytidine deaminase (AID) (2013)
  • Author(s): Watashi K, Liang G, Iwamoto M, Marusawa H, Uchida N, Daito T, Kitamura K, Muramatsu M, Ohashi H, Kiyohara T, Suzuki R, Li J, Tong S, Tanaka Y, Murata K, Aizaki H, Wakita T
  • Journal Title: J Biol Chem Volume: 288 Issue: 44 Pages: 31715-31727
  • DOI: 10.1074/jbc.m113.501122
  • NAID: 120006602140
  • Peer Reviewed
• [Journal Article] 薬学的視点からのウイルス学研究―肝炎ウイルス複製阻害化合物の同定とその作用機序 (2013)
  • Author(s): 渡士幸一
  • Journal Title: 薬学雑誌 Volume: 133 Pages: 1169-1175
  • NAID: 130003382269
  • Peer Reviewed
• [Journal Article] Different mechanisms of hepatitis C virus RNA polymerase activation by cyclophilin A and B in vitro (2012)
  • Author(s): Weng L, Tian X, Gao Y, Watashi K, Shimotohno K, Wakita T, Kohara M, Toyoda T
  • Journal Title: Biochim Biophys Acta Volume: 1820 Pages: 1886-1892
• [Journal Article] Japanese reference panel of blood specimens for evaluation of hepatitis C virus RNA and core antigen quantitative assays (2012)
  • Author(s): Murayama A, Sugiyama N, Watashi K, Masaki T, Suzuki R, Aizaki H, Mizuochi T, Wakita T, Kato T
  • Journal Title: J Clin Microbiol Volume: 50 Pages: 1943-1949
• [Journal Article] HBV培養細胞系による新規抗ウイルス化合物のスクリーニング (2012)
  • Author(s): 渡士幸一
  • Journal Title: 肝胆膵 Volume: 65 Pages: 611-617
• [Journal Article] Japanese reference panel of blood specimens for evaluation of hepatitis C virus RNA and core antigen quantitative assays. (2012)
  • Author(s): Maruyama, A.
  • Journal Title: J. Clin. Microbiol. Volume: 50(6) Issue: 6 Pages: 1943-1949
  • DOI: 10.1128/jcm.00487-12
  • Peer Reviewed
• [Journal Article] Potent and selective inhibition of hepatitis C virus replication by novel phenanthridinone derivatives (2011)
  • Author(s): Salim MT, Aoyama H, Sugita K, Watashi K, Wakita T, Hamasaki T, Okamoto M, Urata Y, Hashimoto Y, Baba M
  • Journal Title: Biochem Biophys Res Commun Volume: 415 Pages: 714-719
• [Journal Article] Cyclosporin A associated helicase-like protein facilitates the association of hepatitis C virus RNA polymerase with its cellular cyclophilin B (2011)
  • Author(s): Morohashi K+, Sahara H, Watashi K+, Iwabata K, Sunoki T, Kuramochi K, Takakusagi K, Miyashita H, Sato N, Tanabe A, Shimotohno K, Kobayashi S, Sakaguchi K, Sugawara F. (+equally contributed)
  • Journal Title: PLoS One Volume: 6
• [Journal Article] Cyclosporin A Associated Helicase-Like Protein Facilitates the Association of Hepatitis C Virus RNA Polymerase with Its Cellular Cyclophilin B (2011)
  • Author(s): 諸橋謙吾、佐原広益、小林進、坂口謙吾、菅原二三男
  • Journal Title: PLoS ONE Volume: 6 Issue: 4 Pages: 18285-18285
  • DOI: 10.1371/journal.pone.0018285
  • Peer Reviewed
• [Presentation] B型肝炎ウイルス感染を抑制するサイトカインの同定とその分子メカニズムの解析 (2014)
  • Author(s): 渡士幸一
  • Organizer: Liver Forum in Kyoto
  • Place of Presentation: 京都
• [Presentation] IL-1- and TNFalpha-triggered intracellular immune response against HBV (2013)
  • Author(s): K. Watashi
  • Organizer: 1^<st> Japan-Taiwan Research Symposium on Hepatitis B virus
  • Place of Presentation: Tokyo(Japan)
• [Presentation] Cyclophilin inhibitors potentiate interferon signaling through diminished PKR phosphorylation in HCV-infected cells (2013)
  • Author(s): K. Watashi, T. Daito, A. Sluder, K. Borroto-Esoda, T. Wakita
  • Organizer: European Association for the Study of the Liver 2013
  • Place of Presentation: Amsterdam(Netherlands)
• [Presentation] ウイルス感染系を基盤とした真菌由来天然化合物の生理活性探索 (2013)
  • Author(s): 渡士幸一、中嶋翔
  • Organizer: 天然物ケミカルバイオロジー第4回公開シンポジウム
  • Place of Presentation: つくば
• [Presentation] Novel regulation mechanism of interferon signaling by cyclophilin through modulation of PKR in HCV-infected cells (2013)
  • Author(s): Watashi, T. Daito, A. Sluder, K. Borroto-Esoda, T. Wakita
  • Organizer: 20th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Melbourne(Australia)
• [Presentation] Isolation of a natural product inhibiting the transcriptional activity of liver X receptor and reducing the production of infectious HCV (2013)
  • Author(s): S. Nakajima, K. Watashi, S. Kamisuki, K. Takemoto, R. Suzuki, H. Aizaki, F. Sugawara, T. Wakita
  • Organizer: 20th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Melbourne(Australia)
• [Presentation] Dynamics of the cellular metabolome during hepatitis C virus infection–Regulation of the lipoprotein metabolisms by hepatic lipase - (2013)
  • Author(s): A. Fujimoto, H. Aizaki, M. Matsuda, N. Watanabe, K. Watashi, R. Suzuki, T. Suzuki, T. Miyamura, T. Wakita
  • Organizer: 20th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Melbourne(Australia)
• [Presentation] Cyclosporin A and its analogs inhibit hepatitis B virus entry into cultured hepatocytes through targeting sodium taurocholate cotransporting polypeptide (2013)
  • Author(s): K. Watashi, A. Sluder, S. Matsunaga, Ryo, S. Nakajima, M. Iwamoto, S. Tsukuda, K. Borroto-Esoda, M. Sugiyama, Y. Tanaka, M. Mizokami, T. Wakita
  • Organizer: 2013 International Meeting on Molecular Biology of Hepatitis B viruses
  • Place of Presentation: Shanghai(China)
• [Presentation] Interleukin-1 and tumor necrosis factor-alpha trigger restriction of hepatitis B virus infection via a cytidine deaminase AID (2013)
  • Author(s): K. Watashi, G. Liang, M. Iwamoto, H. Marusawa, K. Kitamura, M. Muramatsu, R. Suzuki, J. Li, S. Tong, Y. Tanaka, K. Murata, H. Aizaki, T. Wakita
  • Organizer: 2013 International Meeting on Molecular Biology of Hepatitis B viruses
  • Place of Presentation: Shanghai(China)
• [Presentation] Mechanistic analysis on hepatitis B virus entry in an NTCP-overexpressing cell line (2013)
  • Author(s): M. Iwamoto, K. Watashi, S. Tsukuda, H. Aly, R. Suzuki, H. Aizaki, O. Koiwai, H. Kusuhara, T. Wakita
  • Organizer: 2013 International Meeting on Molecular Biology of Hepatitis B viruses
  • Place of Presentation: Shanghai(China)
• [Presentation] A retinoid derivative inhibits hepatitis B virus entry mediated by NTCP (2013)
  • Author(s): S. Tsukuda, K. Watashi, M. Iwamoto, R. Suzuki, H. Aizaki, S. Kojima, T. Wakita
  • Organizer: 2013 International Meeting on Molecular Biology of Hepatitis B viruses
  • Place of Presentation: Shanghai(China)
• [Presentation] The discovery of a new virus/host interaction regulating HBV life cycle (2013)
  • Author(s): H. Aly, K. Watashi, K. Chayama, T. Wakita
  • Organizer: 2013 International Meeting on Molecular Biology of Hepatitis B viruses
  • Place of Presentation: Shanghai(China)
• [Presentation] Formation of covalently closed circular (ccc)DNA tetracycline inducible HBV expression cell line (2013)
  • Author(s): N. Ogura, K. Watashi, T. Wakita
  • Organizer: 2013 International Meeting on Molecular Biology of Hepatitis B viruses
  • Place of Presentation: Shanghai(China)
• [Presentation] Analysis of bioactivity of fungal-derived natural products based on a virus infection system (2013)
  • Author(s): S. Nakajima, K. Watashi
  • Organizer: The 2nd International Symposium on Chemical Biology of Natural Products
  • Place of Presentation: Yokohama(Japan)
• [Presentation] シチジンデアミナーゼAID誘導を介した抗B型肝炎ウイルス細胞内免疫応答機構の解明 (2013)
  • Author(s): 渡士幸一、Guoxin Liang、岩本将士、丸澤宏之、喜多村晃一、村松正道、鈴木亮介、相崎英樹、脇田隆字
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] PKR活性化制御を介するシクロフィリン阻害剤の新たな抗C型肝炎ウイルス作用機序 (2013)
  • Author(s): 大東卓史、渡士幸一、Ann Sluder、中嶋翔、Katyna Borroto-Esoda、藤田尚志、脇田隆字
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] C型肝炎ウイルス分泌過程はphospholipase Dが関わる膜輸送により制御される (2013)
  • Author(s): 内田奈々子、渡士幸一、中嶋翔、岩本将士、鈴木亮介、相崎英樹、千葉丈、脇田隆字
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] ヒトNTCP安定発現細胞株におけるB型肝炎ウイルス侵入機構の解析 (2013)
  • Author(s): 岩本将士、渡士幸一、九十田千子、アリフセイン、鈴木亮介、相崎英樹、小祝修、楠原洋之、脇田隆字
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] B型肝炎ウイルス侵入阻害剤の同定およびそのNTCPを介した感染阻害機構の解明 (2013)
  • Author(s): 九十田千子、渡士幸一、岩本将士、鈴木亮介、相崎英樹、小嶋聡一、脇田隆字
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Liver X receptor転写活性および感染性C型肝炎ウイルス粒子産生を阻害する天然化合物の同定 (2013)
  • Author(s): 中嶋翔、渡士幸一、紙透伸治、竹本健二、鈴木亮介、相崎英樹、菅原二三男、脇田隆字
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Study of new virus/host interactions regulating HBV life cycle (2013)
  • Author(s): フセインアリ、渡士幸一、茶山一彰、脇田隆字
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] 日本脳炎ウイルスレプリコンを用いたトランスパッケージング型1回感染性フラビウイルス粒子産生系の開発 (2013)
  • Author(s): 鈴木亮介、石川知弘、小西英二、嵯峨涼平、松田麻未、渡士幸一、相崎英樹、高崎智彦、脇田隆字
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] C型肝炎ウイルス感染による宿主細胞の脂質代謝変化とHepatic Lipase発現制御 (2013)
  • Author(s): 藤本陽、相崎英樹、松田麻未、渡邉則幸、渡士幸一、鈴木亮介、鈴木哲朗、宮村達男、脇田隆字
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] C型肝炎ウイルスNS5A結合膜タンパク質ELAVL1のウイルス複製・翻訳スイッチング機構の解析 (2013)
  • Author(s): 後藤耕司、相崎英樹、渡邉則幸、渡士幸一、鈴木亮介、山越智、四柳宏、森屋恭爾、小池和彦、鈴木哲朗、宮村達男、脇田隆字
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] 細胞内発現抗体(イントラボディ)によるC型肝炎ウイルスの増殖抑制 (2013)
  • Author(s): 松田麻未、斎藤憲司、鈴木亮介、佐藤充、鐘ヶ江裕美、渡士幸一、相崎英樹、千葉丈、斎藤泉、脇田隆字、鈴木哲朗
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] PhospholipaseA2およびAutophagyによるC型肝炎ウイルス(HCV)分泌過程の制御-グリチルリチンによる抗HCV作用- (2013)
  • Author(s): 青柳東代、相崎英樹、松本喜弘、松田麻未、SuSu Hmwe、渡邉則幸、渡士幸一、鈴木亮介、市野瀬志津子、松浦知和、鈴木哲朗、和気健二郎、宮村達男、脇田隆字
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Tetherin/BST-2はHBV複製を負に制御する (2013)
  • Author(s): 古賀れい奈、宮川敬、松永智子、渡士幸一、脇田隆字、梁明秀
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] 新規ピラノンカルボキサミド誘導体の抗HCV効果とその構造活性相関 (2013)
  • Author(s): 馬場昌範、外山政明、伊藤渉、岡本実佳、渡士幸一、脇田隆字、Sharon Ashoke
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] プラスミドトランスフェクションによるトランスパッケージング型1回感染性フラビウイルス産生系の確立 (2013)
  • Author(s): 鈴木亮介、石川知弘、小西英二、嵯峨涼平、松田麻未、渡士幸一、相崎英樹、高崎智彦、脇田隆字
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] PhospholipaseA2およびAutophagyによるC型肝炎ウイルス(HCV)分泌過程の制御-グリチルリチンによる抗HCV作用- (2013)
  • Author(s): 青柳東代、相崎英樹、松本喜弘、松田麻未、SuSu Hmwe、渡邉則幸、渡士幸一、鈴木亮介、市野瀬志津子、松浦知和、鈴木哲朗、和気健二郎、宮村達男、脇田隆字
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 肝炎ウイルス複製を阻害する低分子化合物/生理活性物質の同定とその作用機序 (2013)
  • Author(s): 渡士幸一
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Presentation] IL-1- and TNFalpha-triggered intracellular immune response against HBV (2013)
  • Author(s): K. Watashi
  • Organizer: 1st Japan-Taiwan Research Symposium on Hepatitis B virus
  • Place of Presentation: Tokyo(Japan)
  • Invited
• [Presentation] Dynamics of the cellular metabolome during hepatitis C virus infection – Regulation of the lipoprotein metabolisms by hepatic lipase - (2013)
  • Author(s): A. Fujimoto, H. Aizaki, M. Matsuda, N. Watanabe, K. Watashi, R. Suzuki, T. Suzuki, T. Miyamura, T. Wakita
  • Organizer: 20th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Melbourne(Australia)
• [Presentation] 細胞内発現抗体（イントラボディ）によるC型肝炎ウイルスの増殖抑制 (2013)
  • Author(s): 松田麻未、斎藤憲司、鈴木亮介、佐藤充、鐘ヶ江裕美、渡士幸一、相崎英樹、千葉丈、斎藤泉、脇田隆字、鈴木哲朗
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] 数理モデルによる抗ウイルス薬の薬効評価系の確立 (2013)
  • Author(s): 小泉吉輝、岩見真吾、内田奈々子、脇田隆字、渡士幸一
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] 肝炎ウイルス複製を阻害する低分子化合物／生理活性物質の同定とその作用機序 (2013)
  • Author(s): 渡士幸一
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] 低分子化合物を利用した肝炎ウイルス学解析 (2012)
  • Author(s): 渡士幸一
  • Organizer: 東京大学医科学研究所第一回感染症国際研究センターシンポジウム
  • Place of Presentation: 東京
• [Presentation] 抗HBV剤探索とHBV感染増殖機構の解析 (2012)
  • Author(s): 渡士幸一
  • Organizer: LIVER2012第8回肝免疫・ウイルス・フロンティア肝疾患研究の新潮流
  • Place of Presentation: 東京
• [Presentation] Synthetic studies of MA026, a novel antiviral lipocyclodepsipeptide (2012)
  • Author(s): S. Shimura, M. Ishima, I. Ota, E. Tsutsui, S. kamisuki, H. Murata, T. Yamazaki, T. Suzuki, K. Kuramochi, T. Takeuchi, K. Watashi, S. Kobayashi, F. Sugawara
  • Organizer: International Congress on Natural Products Research 2012
  • Place of Presentation: New York(USA)
• [Presentation] 数理モデルを用いた肝炎ウイルスの解析 (2012)
  • Author(s): 渡士幸一
  • Organizer: 第22回数理生物学会
  • Place of Presentation: 岡山
• [Presentation] Interleukin-1 and tumor necrosis factor-alpha suppressed hepatitis B virus infection through NF-kappaB signaling pathway (2012)
  • Author(s): K. Watashi, N. Uchida, T. Daito, T. Kiyohara, R. Suzuki, H. Aizaki, T. Wakita
  • Organizer: 2012 International Meeting on Molecular Biology of Hepatitis B viruses
  • Place of Presentation: Oxford(England)
• [Presentation] Development of cell clones for analyzing anti-hepatitis B virus compounds (2012)
  • Author(s): K. Watashi, M. Iwamoto, N. Uchida, T. Kiyohara, R. Suzuki, H. Aizaki, T. Wakita
  • Organizer: 2012 International Meeting on Molecular Biology of Hepatitis B viruses
  • Place of Presentation: Oxford(England)
• [Presentation] Characterization of anti-HCV release inhibitors targeting phospholipase D (2012)
  • Author(s): K. Watashi, N. Uchida, M. Saeed, R. Suzuki, H. Aizaki, T. Wakita
  • Organizer: 19th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Venice(Italy)
• [Presentation] Phospholipase D regulates membrane trafficking during hepatitis C virus egress (2012)
  • Author(s): N. Uchida, K. Watashi, R. Suzuki, H. Aizaki, J. Chiba, T. Wakita
  • Organizer: 19th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Venice(Italy)
• [Presentation] Antiviral activity of glycyrrhizin against hepatitis C virus in vitro (2012)
  • Author(s): Y. Matsumoto, N. Watanabe, K. Watashi, R. Suzuki, T. Matsuura, T. Suzuki, T. Miyamura, K. Wake, T. Wakita, H. Aizaki
  • Organizer: 19th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Venice(Italy)
• [Presentation] An alternative endocytosis pathway for the protective entry of hepatitis C virus (2012)
  • Author(s): R. Suzuki, M. Matsuda, K. Watashi, H. Aizaki, Y. Matsuura, T. Suzuki, T. Wakita
  • Organizer: 19th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Venice(Italy)
• [Presentation] Construction of hepatitis C virus genotype 4a clone (2012)
  • Author(s): H.H. Aly, K. Watashi, N. Watanabe, M. Mizokami, T. Kato, T. Wakita
  • Organizer: 19th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Venice(Italy)
• [Presentation] Identification of natural products inhibiting hepatitis C virus infection (2012)
  • Author(s): S. Nakajima, K. Watashi
  • Organizer: The 1st International Symposium on Chemical Biology of Natural Products
  • Place of Presentation: Kyoto(Japan)
• [Presentation] IL-1およびTNF-alphaのB型肝炎ウイルス感染阻害効果 (2012)
  • Author(s): 渡士幸一、内田奈々子、大東卓史、清原知子、鈴木亮介、相崎英樹、脇田隆字
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] Phospholipase D is a cellular regulator during hepatitis C virus egress and a possible target for antiviral strategy (2012)
  • Author(s): K. Watashi, N. Uchida, R. Suzuki, H. Aizaki, T. Wakita
  • Organizer: Japan Society of Hepatology 10th Single Topic Conference
  • Place of Presentation: Tokyo(Japan)
• [Presentation] 抗HBV剤探索とHBV感染増殖機構の解析 (2012)
  • Author(s): 渡士幸一
  • Organizer: LIVER2012 第8回肝免疫・ウイルス・フロンティア 肝疾患研究の新潮流
  • Place of Presentation: 東京
  • Invited
• [Presentation] Synthetic studies of MA026, a novel antiviral lipocyclodepsipeptide (2012)
  • Author(s): S. Shimura, M. Ishima, I. Ota, E. Tsutsui, S. kamisuki, H. Murata, T. Yamazaki, T. Suzuki, K. Kuramochi, T. Takeuchi, K. Watashi, S. Kobayashi, F. Sugawara
  • Organizer: International Congress on Natural Products Research 2012
  • Place of Presentation: New York (USA)
• [Presentation] Interleukin-1 and tumor necrosis factor-alpha suppressed hepatitis B virus infection through NF-kappaB signaling pathway (2012)
  • Author(s): K. Watashi, N. Uchida, T. Daito, T. Kiyohara, R. Suzuki, H. Aizaki, T. Wakita
  • Organizer: 2012 International Meeting on Molecular Biology of Hepatitis B viruses
  • Place of Presentation: Oxford (England)
• [Presentation] Development of cell clones for analyzing anti-hepatitis B virus compounds (2012)
  • Author(s): K. Watashi, M. Iwamoto, N. Uchida, T. Kiyohara, R. Suzuki, H. Aizaki, T. Wakita
  • Organizer: 2012 International Meeting on Molecular Biology of Hepatitis B viruses
  • Place of Presentation: Oxford (England)
• [Presentation] Characterization of anti-HCV release inhibitors targeting phospholipase D (2012)
  • Author(s): K. Watashi, N. Uchida, M. Saeed, R. Suzuki, H. Aizaki, T. Wakita
  • Organizer: 19th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Venice (Italy)
• [Presentation] Phospholipase D regulates membrane trafficking during hepatitis C virus egress (2012)
  • Author(s): N. Uchida, K. Watashi, R. Suzuki, H. Aizaki, J. Chiba, T. Wakita
  • Organizer: 19th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Venice (Italy)
• [Presentation] Antiviral activity of glycyrrhizin against hepatitis C virus in vitro (2012)
  • Author(s): Y. Matsumoto, N. Watanabe, K. Watashi, R. Suzuki, T. Matsuura, T. Suzuki, T. Miyamura, K. Wake, T. Wakita, H. Aizaki
  • Organizer: 19th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Venice (Italy)
• [Presentation] An alternative endocytosis pathway for the protective entry of hepatitis C virus (2012)
  • Author(s): R. Suzuki, M. Matsuda, K. Watashi, H. Aizaki, Y. Matsuura, T. Suzuki, T. Wakita
  • Organizer: 19th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Venice (Italy)
• [Presentation] Construction of hepatitis C virus genotype 4a clone (2012)
  • Author(s): H.H. Aly, K. Watashi, N. Watanabe, M. Mizokami, T. Kato, T. Wakita
  • Organizer: 19th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Venice (Italy)
• [Presentation] Identification of natural products inhibiting hepatitis C virus infection (2012)
  • Author(s): S. Nakajima, K. Watashi
  • Organizer: The 1st International Symposium on Chemical Biology of Natural Products
  • Place of Presentation: 京都
• [Presentation] Phospholipase D is a cellular regulator during hepatitis C virus egress and a possible target for antiviral strategy (2012)
  • Author(s): K. Watashi, N. Uchida, R. Suzuki, H. Aizaki, T. Wakita
  • Organizer: Japan Society of Hepatology 10th Single Topic Conference
  • Place of Presentation: 東京
• [Presentation] Identification and functional analysis of small molecules inhibiting the late step of hepatitis C virus life cycle (2011)
  • Author(s): K. Watashi, N. Uchida, R. Suzuki, H. Aizaki, T. Wakita
  • Organizer: 18th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Seattle, USA
• [Presentation] Identification of a novel cellular RNA helicase-like protein as a target for cyclosporin A that is involved in hepatitis C virus genome replication (2011)
  • Author(s): K. Watashi, H. Sahara, K. Morohashi, K. Iwabata, T. Sunoki, K. Kuramochi, K. Takakusagi, H. Miyashita, N. Sato, A. Tanabe, K. Shimotohno, S. Kobayashi, K. Sakaguchi, F. Sugawara
  • Organizer: 18th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Seattle, USA
• [Presentation] Halopemide inhibited a post-assembly step in hepatitis C virus life cycle (2011)
  • Author(s): N. Uchida, K. Watashi, R. Suzuki, H. Aizaki, J. Chiba, T. Wakita
  • Organizer: 18th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Seattle, USA
• [Presentation] Identification of lipid droplet-associated membrane proteins that are involved in HCV production (2011)
  • Author(s): H. Aizaki, Y. Matsumoto, K. Goto, K. Watashi, R. Suzuki, M. Fukasawa, K. Hanada, S. Sato, N. Takahashi, Y. Matsuura, K. Motojima, T. Miyamura, T. Suzuki, T. Wakita
  • Organizer: 18th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Seattle, USA
• [Presentation] Identification of novel NS5A-associated proteins in the host-cell membrane fraction and their International Symposium on hepatitis C virus and related viruses (2011)
  • Author(s): K. Goto, T. Kimura, K. Watashi, R. Suzuki, S. Yamagoe, T. Miyamura, K. Moriya, H. Yotsuyanagi, K. Koike, T. Suzuki, T. Wakita, H. Aizaki
  • Organizer: 18th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Seattle, USA
• [Presentation] Signal peptidase complex 1 participates in the assembly of hepatitis C virus through an interaction with NS2 (2011)
  • Author(s): R. Suzuki, T. Suzuki, K. Saito, M. Matsuda, K. Watashi, Y. Matsuura, T. Wakita, H. Aizaki
  • Organizer: 18th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Seattle, USA
• [Presentation] Identification of small molecules affecting late steps of hepatitis C virus life cycle (2011)
  • Author(s): K. Watashi, N. Uchida, R. Suzuki, H. Aizaki, T. Wakita
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
• [Presentation] Identification of a host factor that interacts with hepatitis C virus NS2 protein and is involved in the viral assembly (2011)
  • Author(s): R. Suzuki, T. Suzuki, K. Saito, M. Matsuda, K. Watashi, Y. Matsuura, T. Wakita, H. Aizaki
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
• [Presentation] Antiviral activity of glycyrrhizin against hepatitis C virus in vitro (2011)
  • Author(s): Y. Matsumoto, K. Watashi, R. Suzuki, T. Matsuura, T. Suzuki, T. Miyamura, K. Wake, T. Wakita, H. Aizaki
  • Organizer: International Union of Microbiological Societies 2011 Congres
  • Place of Presentation: Sapporo
• [Presentation] Screening of small molecules affecting the production of hepatitis B virus (2011)
  • Author(s): K. Watashi, N. Uchida, R. Suzuki, H. Aizaki, T. Wakita
  • Organizer: 2011 International Meeting on Molecular Biology of Hepatitis B viruses
  • Place of Presentation: Lake Buena Vista, USA
• [Presentation] 数理モデルと肝炎ウイルス解析 (2011)
  • Author(s): 渡士幸一
  • Organizer: 第8回生物数学の理論とその応用
  • Place of Presentation: 京都
• [Presentation] 低分子化合物を利用した肝炎ウイルス学解析 (2011)
  • Author(s): 渡士幸一
  • Organizer: 感染・免疫・炎症・発癌
  • Place of Presentation: 札幌
• [Presentation] Identification of novel NS5A-associated proteins in the host-cell membrane fraction and their role in HCV life cycle (2011)
  • Author(s): Goto K, Kimura T, Watashi K, Suzuki R, Yamagoe S, Miyamura T, Moriya K, Yotsuyanagi H, Koike K, Suzuki T, Wakita T, Aizaki H
  • Organizer: 18th International Symposium on hepatitis C virus and related viruses
  • Place of Presentation: Seattle, USA
• [Presentation] Identification of small molecules affecting late steps of hepatitis C virus life cycle (2011)
  • Author(s): Watashi K, Uchida N, Suzuki R, Aizaki H, Wakita T
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] Identification of a host factor that interacts with hepatitis C virus NS2 protein and is involved in the viral assembly (2011)
  • Author(s): Suzuki R, Suzuki T, Saito K, Matsuda M, Watashi K, Matsuura Y, Wakita T, Aizaki H
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] Antiviral activity of glycyrrhizin against hepatitis C virus in vitro (2011)
  • Author(s): Matsumoto Y, Watashi K, Suzuki R, Matsuura T, Suzuki T, Miyamura T, Wake K, Wakita T, Aizaki H. Antiviral activity of glycyrrhizin against hepatitis C virus in vitro
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] 数理モデルと肝炎ウイルス解析 (2011)
  • Author(s): 渡士幸一
  • Organizer: 第8回生物数学の理論とその応用(招待講演)
  • Place of Presentation: 京都
• [Presentation] 低分子化合物を利用した肝炎ウイルス学解析 (2011)
  • Author(s): 渡士幸一
  • Organizer: 感染・免疫・炎症・発癌シンポジウム(招待講演)
  • Place of Presentation: 札幌
• [Book] 肝疾患Review2014-2015 (2014)
  • Author(s): 渡士幸一
  • Publisher: 日本メディカルセンター
• [Book] エッセンシャルウイルス学 (2014)
  • Author(s): 渡士幸一
  • Publisher: 南江堂
• [Book] 肝疾患Review2014-2015
  • Author(s): 渡士幸一
  • Publisher: 日本メディカルセンター(印刷中)
• [Book] エッセンシャルウイルス学
  • Author(s): 渡士幸一
  • Publisher: 南江堂(印刷中)
• [Remarks] 渡士幸一、日本肝臓学会第10回シングルトピックカンファレンスYoung Investigator Award (2012)
• [Remarks] 渡士幸一、肝炎ウイルス研究財団研究奨励(2012)