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Lympho-epithelial interactions in the thymus for T-cell repertoire formation

Research Project

Project/Area Number 23790540
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Immunology
Research InstitutionResearch Institute, International Medical Center of Japan

Principal Investigator

NITTA Takeshi  独立行政法人国立国際医療研究センター, 研究所, 室長 (30373343)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords獲得免疫 / 胸腺 / 免疫 / T細胞 / 胸腺上皮細胞
Research Abstract

Distinct subsets of thymic epithelial cells support development of repertoire selection of T cells. We found that cortical thymic epithelial cells form multicellular complexes that closely associate with many viable thymocytes. The complexes that completely enclose thymocytes were found to be identical to previously described “thymic nurse cells (TNCs)”. Our results showed that the formation of TNC complexes requires pre-selected CD4^+CD8^+ thymocytes in postnatal thymus. Cells within TNC complexes were enriched for long-lived CD4^+CD8^+ thymocytes that have undergone secondary TCRα rearrangement. These results indicate that cortical thymic epithelial cells interact with pre-selected CD4^+CD8^+ thymocytes to form TNC complexes that provide a microenvironment supporting secondary TCRα rearrangement and T-cell selection.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (9 results) (of which Invited: 1 results)

  • [Journal Article] The development of T lymphocytes in fetal thymus organ culture2013

    • Author(s)
      Nitta T, Ohigashi I, Takahama Y.
    • Journal Title

      Methods Mol Biol

      Volume: 946 Pages: 85-102

    • DOI

      10.1007/978-1-62703-128-8_6

    • ISBN
      9781627031271, 9781627031288
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thymic nurse cells provide microenvironment for secondary TCRα rearrangement in cortical thymocytes2012

    • Author(s)
      Nakagawa Y
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 109 Issue: 50 Pages: 20572-20577

    • DOI

      10.1073/pnas.1213069109

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] miRNAs control the maintenance of thymic epithelia and their competence for T lineage committment and thymocyte selection2012

    • Author(s)
      Zuklys S
    • Journal Title

      J Immunol

      Volume: 189 Issue: 8 Pages: 3894-3904

    • DOI

      10.4049/jimmunol.1200783

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rank signaling links the development of invariant γδ T cell progenitors and Aire+ medullary epithelium2012

    • Author(s)
      Roberts NM, White AJ, Jenkinson WE, Turchinovich G, Nakamura K, Withers DR, McConnell FM, Desanti GE, Benezech C, Parnell SM, Cunningham AF, Paolino M, Penninger J, Simon K, Nitta T, Ohigashi I, Takahama Y, Caamano JH, Hayday AC, Lane PJ, Jenkinson EJ, Anderson G
    • Journal Title

      Immunity

      Volume: 36 Issue: 3 Pages: 427-437

    • DOI

      10.1016/j.immuni.2012.01.016

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] CCR7 with S1P1 signaling through AP1 for migration of Foxp3+ regulatory T cells controls autoimmune exocrinopathy2012

    • Author(s)
      Ishimaru N
    • Journal Title

      American Journal of Pathology

      Volume: 180 Issue: 1 Pages: 199-208

    • DOI

      10.1016/j.ajpath.2011.09.027

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of RANKL on the thymic medulla2011

    • Author(s)
      Ohigashi I
    • Journal Title

      European Journal of Immunology

      Volume: 41 Issue: 7 Pages: 1822-1827

    • DOI

      10.1002/eji.201141480

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 胸腺皮質微小環境の形成と機能2013

    • Author(s)
      新田 剛
    • Organizer
      第10回Osteoimmunology Forum
    • Place of Presentation
      東京
    • Year and Date
      2013-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] T 細胞分化におけるThemis 分子内ドメインの役割2012

    • Author(s)
      新田 剛、岡田季之、加地健太郎、小田浩代、高島明子、Michael S. Patrick、鈴木春巳
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Distinct protein motifs in Themis regulate positive selection of T cells2012

    • Author(s)
      Takeshi Nitta, Toshiyuki Okada, Hiroyo Oda, Akiko Takashima, Michael S. Patrick, Harumi Suzuki
    • Organizer
      第41回日本免疫学会学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2012-12-06
    • Related Report
      2012 Final Research Report
  • [Presentation] Themis 分子内ドメインの機能解析2012

    • Author(s)
      新田 剛、岡田季之、加地健太郎、小田 浩代、高島明子、Michael S. Patrick、鈴木春巳
    • Organizer
      第22回 Kyoto T Cell Conference
    • Place of Presentation
      京都
    • Year and Date
      2012-07-06
    • Related Report
      2012 Final Research Report
  • [Presentation] Lympho-epithelial complexes in the thymic cortex2011

    • Author(s)
      Takeshi Nitta, Izumi Ohigashi, Yasushi Nakagawa, Yousuke Takahama
    • Organizer
      第40回日本免疫学会総会・学術集会
    • Place of Presentation
      Chiba, Japan
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 胸腺皮質上皮細胞と胸腺細胞の多細胞複合体2011

    • Author(s)
      新田 剛、新田幸子、中川靖士、高浜洋介
    • Organizer
      第21回 Kyoto T Cell Conference
    • Place of Presentation
      京都
    • Year and Date
      2011-06-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Lympho-epithelial complexes in the thymic cortex.2011

    • Author(s)
      Takeshi Nitta
    • Organizer
      第40回日本免疫学会総会・学術集会(招待講演)
    • Place of Presentation
      千葉市
    • Related Report
      2011 Research-status Report
  • [Presentation] 胸腺皮質上皮細胞と胸腺細胞の多細胞複合体2011

    • Author(s)
      新田 剛
    • Organizer
      第21回 Kyoto T Cell Conference
    • Place of Presentation
      京都市
    • Related Report
      2011 Research-status Report
  • [Presentation] 胸腺皮質微小環境の形成と機能

    • Author(s)
      新田 剛
    • Organizer
      第10回 Osteoimmunology Forum
    • Place of Presentation
      東京都文京区
    • Related Report
      2012 Annual Research Report
    • Invited

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Published: 2011-08-05   Modified: 2019-07-29  

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