The development of novel colorectal cancer screening method using exosomal antisense RNA in serum

Research Project

Project/Area Number	23790613
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Laboratory medicine
Research Institution	Hirosaki University
Principal Investigator	CHIBA Mitsuru 弘前大学, 大学院・保健学研究科, 助教 (20583735)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	腫瘍検査学 / アンチセンス RNA / エキソソーム / RNA / 大腸癌 / アンチセンスRNA / スクリーニング
Research Abstract	Exosomes are microvesicles that are released from various cells into the extracellular space. It has been reported that the components within exosomes vary according to the type of secreted cell. In the present study, we identified a species of mRNAs, microRNAs, and natural antisense RNAs within the exosomes secreted from the three colorectal cancer (CRC) cell lines. We discovered that their natural antisense RNAs were within exosomes for the first time in the present study. Currently, the searching of exosomal CRC-specific natural antisense RNAs in serum has been performed.