An analysis of the molecular pathogenesis of heparin-induced thrombocytopenia in the development of a novel clinical assay

• Project/Area Number: 23790615
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: The University of Tokyo
• Principal Investigator: MAKOTO Kaneko 東京大学, 医学部附属病院, 助教 (00377491)
• Research Collaborator: KANNO Nobuko 東京大学, 臨床検査技師 ; WANAKA Keiko 東京大学, 客員研究員 ; NONOBE Ryoko 東京大学, 臨床検査技師
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: ヘパリン起因性血小板減少症 / HIT抗体機能検査法 / セロトニン放出試験 / 活性化血小板 / 臨床検査 / 血小板機能検査 / HIT抗体 / 検査法 / 血小板 / 血小板凝集能

Research Abstract

The responsiveness of platelets isolated from healthy subjects to platelet activating antibodies that develop during heparin-induced thrombocytopenia (HIT) is affected by the activation sensitivity difference of their platelets. Suitable platelets donor samples for use in HIT functional assays can be selected following the confirmation of the sensitivity of platelet activation using both releasing markers from platelets and flow cytometer of platelets. A novel serotonin release assay, i.e., a representative functional HIT antibody (Ab) detection assay, was established using an HPLC-based serotonin level measurement method, without the use of a radioisotope. This novel assay is useful for the detection of functional HIT Abs.

Research Products

• [Journal Article] The diagnosis and management of heparin- induced thrombocytopenia in Japan (2014)
  • Author(s): Makoto Kaneko, Keiko Wanaka, Nelson H. Tsuno, Nobuko Kanno, Ryoko Nonobe, Chiharu Takahashi, Takefumi Matsuo, Hitoshi Okazaki, Yutaka Yatomi
  • Journal Title: ISBT Science Series Volume: (inpress)
  • Peer Reviewed
• [Journal Article] The diagnosis and management of heparin-induced thrombocytopenia in Japan (2014)
  • Author(s): Makoto Kaneko, Keiko Wanaka, Nelson H. Tsuno, Nobuko Kanno, Ryoko Nonobe, Chiharu Takahashi, Takefumi Matsuo, Hitoshi Okazaki, Yutaka Yatomi
  • Journal Title: ISBT Science Series (2014) Volume: in press
  • Peer Reviewed
• [Presentation] HIT診断適正化の現状と今後の動向 (2014)
  • Author(s): 金子誠,菅野信子,高橋千春,野々部亮子,矢冨裕
  • Organizer: 2014年日本血栓止血学会SSCシンポジウムHIT部会
  • Place of Presentation: 東京
  • Year and Date: 2014-02-22
• [Presentation] ヘパリン起因性血小板減少症に対する臨床診断(4T' Sスコア)と免疫学的測定結果との比較 (2013)
  • Author(s): 金子誠,和中敬子,菅野信子,金子知枝子,宮下久美子,久米幸夫,松尾武文,矢冨裕
  • Organizer: 第35回日本血栓止血学学術集会
  • Place of Presentation: 山形
• [Presentation] ラテックス凝集法による抗血小板第4因子/ヘパリン複合体抗体測定の評価 (2013)
  • Author(s): 野々部亮子,金子誠,菅野信子,久米幸夫,小野佳一,横田浩充,矢冨裕
  • Organizer: 第14回日本検査血液学会学術集会
  • Place of Presentation: 東京
• [Presentation] Heparin- induced thrombocytopenia in Japan (2013)
  • Author(s): Makoto Kaneko, Keiko Wanaka, Yutaka Yatomi Y
  • Organizer: 24th Regional Congress of the International Society Blood Transfusion (ISBT)
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Invited
• [Presentation] ヘパリン起因性血小板減少症に対する臨床診断(4T'Sスコア)と免疫学的測定結果との比較 (2013)
  • Author(s): 金子 誠, 和中 敬子, 菅野 信子, 金子 知枝子, 宮下 久美子, 久米 幸夫, 松尾 武文, 矢冨 裕
  • Organizer: 第35回日本血栓止血学学術集会．
  • Place of Presentation: 山形
• [Presentation] Heparin-induced thrombocytopenia in Japan (2013)
  • Author(s): Kaneko M, Wanaka K and Yatomi Y
  • Organizer: 24th Regional Congress of the International Society Blood Transfusion (ISBT)
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Invited
• [Presentation] ヘパリン惹起血小板凝集試験におけるHIT抗体の健常者血小板に対する感受性の差異 (2012)
  • Author(s): 金子誠,菅野信子,鈴木明子,矢冨裕
  • Organizer: 第34回日本血栓止血学学術集会
  • Place of Presentation: 東京
• [Presentation] Difference in the Sensitivity of Donor- Platelet Responsiveness of Anti- Heparin/ Platelet Factor 4 Complex Antibody in Heparin- induced Platelet Aggregation Assay (2012)
  • Author(s): Makoto Kaneko, Nobuko Kanno, Ryoko Tanaka, Akiko Suzuki, Yutaka Yatomi
  • Organizer: 7th Congress of the Asian- Pacific Society on Thrombosis and Haemostasis
  • Place of Presentation: Melbourne, Australia
• [Presentation] ヘパリン惹起血小板凝集試験におけるHIT抗体の健常者血小板に対する感受性の差異． (2012)
  • Author(s): 金子 誠，菅野 信子，鈴木 明子，矢冨 裕．
  • Organizer: 第34回日本血栓止血学学術集会．
  • Place of Presentation: 東京
• [Presentation] Difference in the Sensitivity of Donor-Platelet Responsiveness of Anti-Heparin/Platelet Factor 4 Complex Antibody in Heparin-induced Platelet Aggregation Assay. (2012)
  • Author(s): Kaneko, M., Kanno, N., Tanaka, R., Suzuki, A., and Yatomi, Y.
  • Organizer: 7th Congress of the Asian-Pacific Society on Thrombosis and Haemostasis.
  • Place of Presentation: Melbourne, Australia
• [Remarks]
  • URL: http://lab-tky.umin.jp/research/index.html