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Development of rapid generation of antibodies with high affinity for improvement of detection limi

Research Project

Project/Area Number 23790617
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionUniversity of Toyama

Principal Investigator

OZAWA Tatsuhiko  富山大学, 大学院・医学薬学研究部(医学), 助教 (10432105)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords臨床検査医学 / 高親和性抗体 / ウサギモノクローナル抗体 / ISAAC / 抗体医薬
Research Abstract

Antigen-specific rabbit monoclonal antibodies (RaMoAbs) are useful due to their high specificity and high affinity, and the establishment of a comprehensive and rapid RaMoAb generation system has been highly anticipated. Here, we present a novel system for rapid RaMoAb generation and produce antigen-specific RaMoAbs with high affinity within a time period of only 7 days. We have used this system to efficiently generate RaMoAbs that are specific to a phosphorylated signal-transducing molecule. Our system provides a new method for the comprehensive and rapid production of RaMoAbs, which may contribute to laboratory research and clinical applications.交付決定額

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (43 results)

All 2013 2012 2011

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (25 results) Book (8 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Reduced Level of the BCL11B Protein Is Associated with Adult T-Cell Leukemia/Lymphoma2013

    • Author(s)
      Kurosawa, N.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 1 Pages: e55147-e55147

    • DOI

      10.1371/journal.pone.0055147

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel rabbit immunospot array assay on a chip allows for the rapid generation of rabbit monoclonal antibodies with high affinity.2012

    • Author(s)
      Ozawa T., et al
    • Journal Title

      PLoS One

      Volume: 7 Issue: 12 Pages: e52383-e52383

    • DOI

      10.1371/journal.pone.0052383

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The binding affinity of a soluble TCR-Fc fusion protein is significantly improved by crosslinkage with an anti-Cbeta antibody2012

    • Author(s)
      Ozawa T, Horii M, Kobayashi E, Jin A, Kishi H, Muraguchi A
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 422 Pages: 245-249

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unbiased analysis of TCRα/β chains at the single-cell level in human CD8<sup>+</sup> T-cell subsets2012

    • Author(s)
      Sun X, Saito M, Sato Y, Chikata T, Naruto T, Ozawa T, Kobayashi E, Kishi H, Muraguchi A, Takiguchi M
    • Journal Title

      PLoS One

      Volume: 7 Issue: 7 Pages: e40386-e40386

    • DOI

      10.1371/journal.pone.0040386

    • URL

      https://pure.teikyo.jp/en/publications/4de66c37-9be7-48f7-8a21-dd8a125a0565

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] HELIOS-BCL11B fusion gene involvement in a t(2;14)(q34;q32) in an adult T-cell leukemia patient2012

    • Author(s)
      Fujimoto R, Ozawa T, Itoyama T, Sadamori N, Kurosawa N, Isobe M
    • Journal Title

      Cancer Genet

      Volume: 205 Pages: 356-364

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The binding affinity of a soluble TCR-Fc fusion protein is significantly improved by crosslinkage with an anti-Cβ antibody2012

    • Author(s)
      Ozawa T ., Horii M., Kobayashi E., Jin A., Kishi H., and Muraguchi A
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 422 Issue: 2 Pages: 245-249

    • DOI

      10.1016/j.bbrc.2012.04.134

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus.2011

    • Author(s)
      Ozawa T, Jin A, Tajiri K, Takemoto M, Okuda T, Shiraki K, Kishi H, Muraguchi A
    • Journal Title

      Antiviral Res

      Volume: 91 Pages: 283-287

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus2011

    • Author(s)
      Ozawa T, et al
    • Journal Title

      Antiviral Res

      Volume: 91 Pages: 283-287

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rapid isolation of antigen-specific antibody-secreting cells using a chip-based immunospot array2011

    • Author(s)
      JIn A, et al
    • Journal Title

      Nat Protoc

      Volume: 6 Pages: 668-676

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Rabbit ISAAC (rabbit immunospot array assay on a chip) allows for the rapid generation of rabbit monoclonal antibodies with high affinity2013

    • Author(s)
      Piao X, Muraguchi A, Hamana H, Ozawa T, Kobayashi E, Jin A, Kishi H
    • Organizer
      Keystone Symposia (Antibodies as Drugs)
    • Place of Presentation
      Vancouver, British Columbia, Canada
    • Related Report
      2012 Final Research Report
  • [Presentation] Rabbit ISAAC (rabbit immunospot array assay on a chip) allows for the rapid generation of rabbit monoclonal antibodies with high affinity2013

    • Author(s)
      Hamana H
    • Organizer
      Keystone Symposia (Antibodies as Drugs)
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2012 Annual Research Report
  • [Presentation] A novel rabbit immunospot array assay on a chip allows for the rapid generation of rabbit monoclonal antibodies2012

    • Author(s)
      Ozawa T, Piao X, Kobayashi E, Zhou Y, Sakurai H, Andoh T, Jin A, Kishi H.Muraguchi A
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] The binding affinity of a soluble TCR-Fc fusion protein is significantly improved by crosslinkage with an anti-Cβ antibody.2012

    • Author(s)
      Ozawa T, Kobayashi E, Jin A, Kishi H, Muraguchi A
    • Organizer
      第41回日本免疫学会総会・学術集会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] Rapid generation of super-antibodies using rabbit ISAAC2012

    • Author(s)
      Piao X, Ozawa T, Kobayashi E, Kishi H, Muraguchi A.
    • Organizer
      第41回日本免疫学会総会・学術集会
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Cloning of human antigen-specific TCRs can confer the candidates for cancer gene therapy2012

    • Author(s)
      Kobayashi E, Kishi H, Hamana H, Ozawa T, Nakagawa H, Jin A, Muraguchi A.
    • Organizer
      第41回日本免疫学会総会・学術集会
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Detection of antigen-stimulated cytokine-secretion in human T-cells at single cell levels on a live cell chip.2012

    • Author(s)
      Kishi H, Jin A, Kobayashi E, Ozawa T, Hamana H, Muraguchi A.
    • Organizer
      Detection of antigen-stimulated cytokine-secretion in human T-cells at single cell levels on a live cell chip.
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] Comparison of TCR repertoires in EBV-specific T cells detected by three different staining methods.2012

    • Author(s)
      Hamana H, Kobayashi E, Kishi H, Ozawa T, Jin A, Muraguchi A
    • Organizer
      第41回日本免疫学会総会・学術集会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of cellular responses at singel cell levels with a hybrid magnetic microwell array chip2012

    • Author(s)
      Kishi H, Obata T, Takami S, Kazui M, Ozawa T, Ogawa A, Muraguchi A
    • Organizer
      International Joint Symposium on Single-Cell Analysis
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Cloning of human antigen-specific TCRs can confer the candidates for cancer gene therapy2012

    • Author(s)
      Kobayashi E, Mizukoshi E, Kishi H, Hamana H, Nagai T, Ozawa T, Nakagawa H, Jin A, Kaneko S, Muraguchi A
    • Organizer
      SITC 27th Annual Meeting
    • Place of Presentation
      North Bethesda, MD, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] がんのTCR遺伝子治療にむけた候補TCR遺伝子の迅速クローニング法2012

    • Author(s)
      岸裕幸, 小林栄治, 水腰英四郎, 小澤龍彦, 金子周一, 村口篤
    • Organizer
      第71回日本癌学会学術集会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] he binding affinity of a soluble TCR-Fc fusion protein is significantly improved by crosslinkage with an anti-Cβ antibody2012

    • Author(s)
      Ozawa T
    • Organizer
      第41回日本免疫学会総会・学術集会
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] Detection of antigen-stimulated cytokine-secretion in human T-cells at single cell levels on a live cell chip2012

    • Author(s)
      Kishi H
    • Organizer
      第41回日本免疫学会総会・学術集会
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] Comparison of TCR repertoires in EBV-specific T cells detected by three different staining methods2012

    • Author(s)
      Hamana H
    • Organizer
      第41回日本免疫学会総会・学術集会
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cloning of human antigen-specific TCRs can confer the candidates for cancer gene therapy2012

    • Author(s)
      Kobayashi E
    • Organizer
      SITC 27th Annual Meeting
    • Place of Presentation
      North Bethesda, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] A high-throughput method for generating rabbit monoclonal antibodies by immunospot array assay on a chip technology2011

    • Author(s)
      Ozawa T, Piao X, Kobayashi E, Kishi H, Muraguchi A.
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] 可溶化TCRの作製と機能解析2011

    • Author(s)
      小澤龍彦, 小林栄治, 岸裕幸, 村口篤
    • Organizer
      第40回日本免疫学会総会・学術集会
    • Place of Presentation
      千葉
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] The novel method to detect single antibody-secreting B-cells and single cytokine-secreting T-cells using a cell-microarray chip2011

    • Author(s)
      Ozawa T, Kobayashi E, Tajiri K, Jin A, Kishi H, Muraguchi A
    • Organizer
      Cold Spring Harbor Meeting, Single cell analysis
    • Place of Presentation
      New York, USA
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] 単一細胞解析に基づく迅速な抗原特異的T細胞受容体クローニングシステム.2011

    • Author(s)
      Kobayashi E, Hamana H, Nagai T, Ozawa T, Kishi H, Muraguchi A.
    • Organizer
      第40回日本免疫学会総会・学術集会
    • Place of Presentation
      千葉
    • Related Report
      2012 Final Research Report
  • [Presentation] 迅速なTCR cDNAクローニングシステムを用いたEBV特異的単一T細胞のTCRα/βレパトア解析2011

    • Author(s)
      浜名洋, 小林栄治, 長井輝美, 小澤龍彦, 岸裕幸, 村口篤
    • Organizer
      第40回日本免疫学会総会・学術集会
    • Place of Presentation
      千葉
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] ISAAC法を用いた迅速かつハイスループットなウサギモノクローナル抗体作成法の開発2011

    • Author(s)
      朴秀虹, 小澤龍彦, 小林栄治, 岸裕幸, 村口篤.
    • Organizer
      第40回日本免疫学会総会・学術集会
    • Place of Presentation
      千葉
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] 細胞チップを用いた抗原特異的サイトカイン分泌T細胞の単一細胞レベルでの検出2011

    • Author(s)
      Kishi H, Kobayashi E, Ozawa T, Hamana H, Nagai T, Tajiri K, Muraguchi A
    • Organizer
      第40回日本免疫学会総会・学術集会
    • Place of Presentation
      千葉
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] Single T cell analysis system for rapid cloning and functional evaluation of antigen-specific T cell receptors Cold Spring Harbor Conferences Asia2011

    • Author(s)
      Kobayashi E, Hamana H, Nagai T, Horii M, Ozawa T, Kishi H, Muraguchi A.
    • Place of Presentation
      Suzhou, China.
    • Related Report
      2012 Final Research Report
  • [Presentation] 単一細胞解析に基づく迅速な抗原特異的T細胞受容体クローニングシステム2011

    • Author(s)
      Kobayashi E, et al
    • Organizer
      第40回日本免疫学会総会・学術集会
    • Place of Presentation
      千葉
    • Related Report
      2011 Research-status Report
  • [Presentation] Single T cell analysis system for rapid cloning and functional evaluation of antigen-specific T cell receptors2011

    • Author(s)
      Kobayashi E, et al
    • Organizer
      Cold Spring Harbor Conferences Asia
    • Place of Presentation
      Suzhou, China
    • Related Report
      2011 Research-status Report
  • [Book] Screening of antigen-specific antibody-secreting cells2012

    • Author(s)
      Kishi H, Jin A, Ozawa T, Tajiri K, Obata T, Muraguchi A
    • Publisher
      Springer、Methods Mol Biol
    • Related Report
      2012 Final Research Report
  • [Book] Screening of antigen-specific antibody-secreting cells2012

    • Author(s)
      Kishi H. et al
    • Total Pages
      10
    • Publisher
      Humana Press「Methods Mol Biol」
    • Related Report
      2011 Research-status Report
  • [Book] ンパ球アレイ法を用いた抗体医薬の開発. 細胞2011

    • Author(s)
      小澤龍彦, 田尻和人, 岸裕幸, 村口篤
    • Publisher
      ニューサイエンス社
    • Related Report
      2012 Final Research Report
  • [Book] シングル細胞チップと抗体医薬への応用2011

    • Author(s)
      小澤龍彦, 岸裕幸, 村口篤
    • Publisher
      シーエムシー出版、ナノ融合による先進バイオデバイス
    • Related Report
      2012 Final Research Report
  • [Book] 新しい抗体スクリーニング法.2011

    • Author(s)
      小澤龍彦, 岸裕幸, 村口篤
    • Publisher
      医薬の門社、感染・炎症・免疫
    • Related Report
      2012 Final Research Report
  • [Book] 新しい抗体スクリーニング法2011

    • Author(s)
      小澤龍彦 他
    • Total Pages
      9
    • Publisher
      医薬の門社「感染・炎症・免疫」
    • Related Report
      2011 Research-status Report
  • [Book] シングル細胞チップと抗体医薬への応用2011

    • Author(s)
      小澤龍彦 他
    • Total Pages
      8
    • Publisher
      シーエムシー出版「ナノ融合による先進バイオデバイス」
    • Related Report
      2011 Research-status Report
  • [Book] リンパ球アレイ法を用いた抗体医薬の開発2011

    • Author(s)
      小澤龍彦 他
    • Total Pages
      4
    • Publisher
      ニューサイエンス社「細胞」
    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] 抗原特異的ウサギ抗体産生細胞の迅速な特定方法およびその利用2012

    • Inventor(s)
      村口篤, 岸裕幸, 小澤龍彦
    • Industrial Property Rights Holder
      村口篤, 岸裕幸, 小澤龍彦
    • Industrial Property Number
      2012-221822
    • Filing Date
      2012-10-04
    • Related Report
      2012 Annual Research Report 2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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