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Development ofrapid cancer diagnosis by the novel electrospray ionization-mass spectrometry

Research Project

Project/Area Number 23790619
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionUniversity of Yamanashi

Principal Investigator

YOSHIMURA Kentaro  山梨大学, 大学院・医学工学総合研究部, 助教 (70516921)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords質量分析 / 学習機械 / がん診断 / 大気圧 / エレクトロスプレー / ベイズ統計学
Research Abstract

Probe electrospray ionization (PESI) is a biocompatible ambient ionization technique. We determined the conditions of measurements for lipids in human tissues. More than 3,000 spectra datasets were obtained from specimens of human renal cell carcinoma and hepatocellular carcinoma. These data were used as a training dataset, which serves as a reference for the dPLRM. We input randomly selected test datasets to the dPLRM to get probabilistic result of cancer diagnosis. We achieved over 90% coincidence of diagnosis with those judged by the conventional histopathology.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (42 results)

All 2013 2012 2011 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (25 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Characterization of ciliary targeting sequence of rat melanin-concentrating hormone receptor 12013

    • Author(s)
      Nagata A, Hamamoto A, Horikawa M, Yoshimura K
    • Journal Title

      General and Comparative Endocrinology

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biomolecular analysis and cancer diagnostics by negative mode probe electrospray ionization2013

    • Author(s)
      Mandal MK, Saha S, Yoshimura K
    • Journal Title

      Analyst

      Volume: 138 Pages: 1682-8

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of probe electrospray ionization mass spectrometry (PESI-MS) to clinical diagnosis: solvent effect on lipid analysis2013

    • Author(s)
      Mandal MK, Yoshimura K, Chen LC
    • Journal Title

      Journal of The American Society for Mass Spectrometry

      Volume: 23 Pages: 2043-7

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of renal cell carcinoma as a first step for developing mass spectrometry-based diagnostics2012

    • Author(s)
      Yoshimura K, Chen LC, Mandal MK
    • Journal Title

      Journal of The American Society for Mass Spectrometry

      Volume: 23 Pages: 1741-9

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Solid probe assisted nanoelectrospray ionization mass spectrometry for biological tissue diagnostics2012

    • Author(s)
      Mandal MK, Yoshimura K, Saha S
    • Journal Title

      Analyst

      Volume: 137 Pages: 4658-61

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Innovations in Analytical Oncology - Status quo of Mass Spectrometry-Based Diagnostics for Malignant Tumor2012

    • Author(s)
      Takeda S, Yoshimura K, Hiraoka K
    • Journal Title

      Journal of Analytical Oncology

      Volume: 1 Pages: 74-80

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hedgehog signaling regulates myelination in the peripheral nervous system through primary cilia2012

    • Author(s)
      Yoshimura K, Takeda S
    • Journal Title

      Differentiation

      Volume: 83

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of probe electrospray ionization mass spectrometry (PESI-MS) to clinical diagnosis: solvent effect on lipid analysis2012

    • Author(s)
      Mandal MK
    • Journal Title

      Journal of The American Society for Mass Spectrometry

      Volume: 23 Pages: 2043-7

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hedgehog signaling regulates myelination in the peripheral nervous system through primary cilia2012

    • Author(s)
      Yoshimura K
    • Journal Title

      Differentiation

      Volume: 83

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-time analysis of living animals by electrospray ionization mass spectrometry2011

    • Author(s)
      Yoshimura K, Chen LC, Yu Z, Hiraoka K, Takeda S
    • Journal Title

      Analytical Biochemistry

      Volume: 417 Pages: 195-201

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Signaling through the primary cilium affects glial cell survival under a stressed environment2011

    • Author(s)
      Kentaro Yoshimura
    • Journal Title

      Glia

      Volume: 59 Issue: 2 Pages: 333

    • DOI

      10.1002/glia.21105

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Real-time analysis of living animals by electrospray ionization mass spectrometry2011

    • Author(s)
      Kentaro Yoshimura
    • Journal Title

      Anal Biochem

      Volume: 417 Issue: 2 Pages: 195

    • DOI

      10.1016/j.ab.2011.06.020

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hedgehog signaling regulates myelination in the peripheral nervous system through primary cilia2011

    • Author(s)
      Yoshimura, K., Takeda, S.
    • Journal Title

      Differentiation

      Volume: 83 Issue: 2 Pages: S78-S85

    • DOI

      10.1016/j.diff.2011.10.006

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 各種ヘッジホッグシグナルリガンド特異的な生理機能の解析2013

    • Author(s)
      Yoshimura K., Kasai H., Moriishi K., Takeda S
    • Organizer
      第118回日本解剖学会総会・全国学術集会
    • Place of Presentation
      香川
    • Year and Date
      2013-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 各種ヘッジホッグシグナルリガンド特異的な生理機能の解析2013

    • Author(s)
      Yoshimura K
    • Organizer
      第118回日本解剖学会総会・全国学術集会
    • Place of Presentation
      サンポートホール高松・かがわ国際会議場(香川)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 末梢神経のミエリン形成におけるヘッジホッグシグナルの調節機構2012

    • Author(s)
      Yoshimura K., Takeda S
    • Organizer
      第72回日本解剖学会中部地方会
    • Place of Presentation
      岐阜
    • Year and Date
      2012-10-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Developing a human cancer diagnostics system based on the probe electrospray ionization-mass spectrometry and Bayesian statistics2012

    • Author(s)
      Yoshimura K., Chen L.C., Mandal M.K., Tanabe K., Hara M., Fujii H., Takeda M., Hiraoka K., Takeda S
    • Organizer
      19th International Mass Spectrometry Conference
    • Place of Presentation
      京都
    • Year and Date
      2012-09-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Mouse somatostatin receptor 3 on primary cilium modulates the calcium dynamics2012

    • Author(s)
      Yoshimura K., Misawa T., Hamamoto A., Saito Y., Takeda S
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 一次繊毛による末梢ミエリン形成調節2012

    • Author(s)
      Yoshimura K
    • Organizer
      第14回応用薬理シンポジウム
    • Place of Presentation
      山梨
    • Year and Date
      2012-09-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 末梢ミエリン形成における一次繊毛によるHhシグナル受容調節機構2012

    • Author(s)
      Yoshimura K., Takeda S.
    • Organizer
      第3回繊毛研究会
    • Place of Presentation
      東京
    • Year and Date
      2012-06-09
    • Related Report
      2012 Final Research Report
  • [Presentation] 一次繊毛を介したHhシグナルの活性化によるミエリン形成促進機構2012

    • Author(s)
      Yoshimura K., Takeda S
    • Organizer
      第117回日本解剖学会総会合同大会
    • Place of Presentation
      山梨
    • Year and Date
      2012-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 末梢神経のミエリン形成におけるヘッジホッグシグナルの調節機構2012

    • Author(s)
      Yoshimura K
    • Organizer
      第72回日本解剖学会中部地方会
    • Place of Presentation
      じゅうろくプラザ(岐阜)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mouse somatostatin receptor 3 on primary cilium modulates the calcium dynamics2012

    • Author(s)
      Yoshimura K
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Developing a human cancer diagnostics system based on the probe electrospray ionization-mass spectrometry and Bayesian statistics2012

    • Author(s)
      Yoshimura K
    • Organizer
      19th International Mass Spectrometry Conference
    • Place of Presentation
      Kyoto International Conference Center(京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 一次繊毛による末梢ミエリン形成調節2012

    • Author(s)
      Yoshimura K
    • Organizer
      第14回応用薬理シンポジウム
    • Place of Presentation
      ベルクラシック甲府(山梨)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 末梢ミエリン形成における一次繊毛によるHhシグナル受容調節機構2012

    • Author(s)
      Yoshimura K
    • Organizer
      第3回繊毛研究会
    • Place of Presentation
      東京大学理学部2号館大講義室(東京)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 一次繊毛を介したHhシグナルの活性化によるミエリン形成促進機構2012

    • Author(s)
      吉村健太郎
    • Organizer
      第117回日本解剖学会総会合同大会
    • Place of Presentation
      山梨大学 (山梨)
    • Related Report
      2011 Research-status Report
  • [Presentation] 一次繊毛によるヘッジホッグシグナルの受容とシュワン細胞の動態の調節2012

    • Author(s)
      有松朋之
    • Organizer
      第117回日本解剖学会総会合同大会
    • Place of Presentation
      山梨大学 (山梨)
    • Related Report
      2011 Research-status Report
  • [Presentation] Hedgehog signaling facilitates the myelination by Schwann cells through the primary cilia2011

    • Author(s)
      Yoshimura K., Takeda S
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2011-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Rapid cancer diagnosis using electrospray ionization mass spectrometry2011

    • Author(s)
      Yoshimura K., Chen L.C., Mandal M.K., Hiraoka K., Takeda S.
    • Organizer
      第59回質量分析総合討論会
    • Place of Presentation
      大阪
    • Year and Date
      2011-09-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Hedgehog signaling regulates the early stage of myelination by Schwann cells through the primary cilia2011

    • Author(s)
      Yoshimura K., Takeda S.
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道
    • Year and Date
      2011-06-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Real time analysis of living animals by electrospray ionization mass spectrometry using solid needle as sampling probe2011

    • Author(s)
      Yoshimura K., Chen L.C., Zhan Y., Hiraoka K., Takeda S
    • Organizer
      59th American Society for Mass Spectrometry Conference
    • Place of Presentation
      Denver, CO
    • Year and Date
      2011-06-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Rapid Screening of Renal Cell Carcinoma (RCC) by Probe Electrospray Ionization Mass Spectrometry2011

    • Author(s)
      Mridul Kanti Mandal
    • Organizer
      第59回 質量分析総合討論会
    • Place of Presentation
      ホテル阪急エキスポパーク (大阪)
    • Related Report
      2011 Research-status Report
  • [Presentation] Direct Analysis of Biomolecules by Probe Electrospray Ionization Mass Spectrometry2011

    • Author(s)
      Mridul Kanti Mandal
    • Organizer
      第59回 質量分析総合討論会
    • Place of Presentation
      ホテル阪急エキスポパーク (大阪)
    • Related Report
      2011 Research-status Report
  • [Presentation] Rapid cancer diagnosis using electrospray ionization mass spectrometry2011

    • Author(s)
      Kentaro Yoshimura
    • Organizer
      第59回 質量分析総合討論会
    • Place of Presentation
      ホテル阪急エキスポパーク (大阪)
    • Related Report
      2011 Research-status Report
  • [Presentation] Hedgehog signaling facilitates the myelination by Schwann cells through the primary cilia2011

    • Author(s)
      吉村健太郎
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜 (横浜)
    • Related Report
      2011 Research-status Report
  • [Presentation] Hedgehog signaling regulates the early stage of myelination by Schwann cells through the primary cilia2011

    • Author(s)
      吉村健太郎
    • Organizer
      第63回 日本細胞生物学会大会
    • Place of Presentation
      北海道大学 (札幌)
    • Related Report
      2011 Research-status Report
  • [Presentation] Real time analysis of living animals by electrospray ionization mass spectrometry using solid needle as sampling probe2011

    • Author(s)
      Kentaro Yoshimura
    • Organizer
      59th American Society for Mass Spectrometry Conference
    • Place of Presentation
      Colorado Convention Center (Denver)
    • Related Report
      2011 Research-status Report
  • [Remarks] 山梨大学研究者総覧

    • URL

      http://erdb.yamanashi.ac.jp/rdb/A_DispInfo.Scholar/3_51/39A95025371B8030.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.cellbiology.jp/

    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] 質量分析装置、及び該装置を用いた癌診断装置2012

    • Inventor(s)
      出水 秀明、谷畑 博司、糸井 弘人、田邉 國士、竹田 扇、吉村 健太郎、平岡 賢三、チェン リー チュイン、堀 裕和
    • Industrial Property Rights Holder
      株式会社島津製作所、田邉 國士、国立大学法人山梨大学
    • Filing Date
      2012-08-27
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 質量分析装置、及び該装置を用いた癌診断装置2012

    • Inventor(s)
      出水 秀明、谷畑 博司、田邉 國士、竹田 扇、吉村 健太郎
    • Industrial Property Rights Holder
      株式会社島津製作所、田邉 國士、国立大学法人山梨大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-186490
    • Filing Date
      2012-08-27
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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