Discovery of novel autoantibodies of fulminant type 1diabetes and autoimmune pancreatitis, the establishment of a measurement system.

• Project/Area Number: 23790620
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: University of Yamanashi
• Principal Investigator: TAKIZAWA Soichi 山梨大学, 医学工学総合研究部, 助教 (80456467)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 免疫血清学 / 免疫学 / 糖尿病

Research Abstract

We screened the human pancreas cDNA library using serum of theautoimmune pancreatitis with diabetes. I reported the new autoantibody which were common to autoimmune pancreatitis and fulminant type 1 diabetes mellitus by a preliminary experiment. I built the Enzyme-linked immunosorbent assay (ELISA) for detecting autoantibody against human amylase. As a result of having triedidentification of the epitope of the amylase antibody, I was able to pinpoint the part that antigenicity was high in. I try to build the ELISA having high sensitivity and specificity more now.