Proteomic-based identification of renal tubular markers related to the new mechanism of urinary albumin excretion.

• Project/Area Number: 23790627
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Bunkyo Gakuin University
• Principal Investigator: NAKAYAMA Aki 文京学院大学, 保健医療技術学部 臨床検査学科, 助手 (00599425)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 尿中アルブミン / Tamm-Horsfall protein / エキソソーム / 尿中Tamm-Horsfall protein / 尿中エキソソーム / 尿中タンパク質

Research Abstract

The urinary albumin peptides metabolized in the renal tubular cells and excreted into urine, and which is considered as an in-direct tubular marker were identified by proteome analysis. The quantitative analysis of urinary Tamm-Horsfall protein showed that the significant decrease level in the patients with renal dysfunction. The proteome analysis of urinary exosomes enabled the identification of variety types of protein derived from renal epithelial cells. These results provide the basic information to predict the tubular dysfunction in renal disease condition.

Research Products

• [Journal Article] Comparison of urinary exosomal protein solubilization methods for two-dimensional gel electrophoresis (2012)
  • Author(s): Kosaka A, Nakayama A, Yamaguchi I, Kasama T, Totsuka M, Shiba K
  • Journal Title: J Electrophoresis Volume: 56 Pages: 7-11
  • NAID: 130001910791
  • Peer Reviewed
• [Journal Article] Comparison of urinary exosomal protein solubilization methods for two-dimensional gel electrophoresis (2012)
  • Author(s): Kosaka A, Nakayama A, Yamaguchi I, Kasama T, Totsuka M, Shiba K.
  • Journal Title: Journal of Electrophoresis Volume: 56 Pages: 7-11
  • NAID: 130001910791
  • Peer Reviewed
• [Journal Article] Redox State of Urinary Albumin in Patients with IgA Nephropathy (2011)
  • Author(s): Nakayama A, Odake J, Kanke A, Sakatsume M, Kasama T, Shiba K
  • Journal Title: Rinsho Byori Volume: 59 Pages: 1013-18
  • NAID: 10030462814
  • Peer Reviewed
• [Journal Article] アルブミン分子多様性の解析 (2011)
  • Author(s): 中山亜紀
  • Journal Title: 生物試料分析 Volume: 34 Pages: 120-125
• [Journal Article] Redox State of Urinary Albumin in Patients with IgA Nephropathy (2011)
  • Author(s): Nakayama A, Odake J, Kanke A, Sakatsume M, Kasama T, Shiba K.
  • Journal Title: Rinsho byori Volume: 59 Pages: 1013-18
  • NAID: 10030462814
  • Peer Reviewed
• [Presentation] 健常人尿中エキソソームタンパク質の基礎的解析 2 (2013)
  • Author(s): 中山亜紀、荒井勇輝、久保田亮、片山映、鈴木秀典、飯島史朗、芝紀代子
  • Organizer: 生物試料分析学会 第23回年次学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2013-02-10
• [Presentation] 妊婦の尿中蛋白解析 -Tamm-Horsfall 蛋白(THP)- (2013)
  • Author(s): 酒井伸枝、久保田亮、中山亜紀、吉越和江、北川優、松本二朗
  • Organizer: 生物試料分析学会 第23回年次学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2013-02-10
• [Presentation] 健常人尿中エキソソームタンパク質の基礎的解析2 (2013)
  • Author(s): 中山亜紀、 荒井勇輝、 久保田亮、片山映、鈴木秀典、飯島史朗、芝紀代子
  • Organizer: 生物試料分析学会 第23回年次学術集会
  • Place of Presentation: 大阪
• [Presentation] Establishment of enzyme-linked immunosorbent assay for urinary Tamm-Horsfall protein (2012)
  • Author(s): Nakayama A, Kubota R, Sakatsume M, Iijima S, Kiyoko Shiba
  • Organizer: 12th meeting of the Asian Society of Clinical pathology and Laboratory Medicine
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2012-11-30
• [Presentation] セルロースアセテート膜電気泳動法を用いた IgA 腎症患者尿中タンパク分画の多様性の解析 (2012)
  • Author(s): 君野舞、中山亜紀、坂爪実、笠間健嗣、芝紀代子、飯島史朗
  • Organizer: 第59回日本臨床検査医学会学術集会
  • Year and Date: 2012-11-29
• [Presentation] Identification of urinary exosomal proteins by two dimensional gel electrophoresis (2012)
  • Author(s): Nakayama A, Kubota R, Arai Y, Katayama A, Suzuki H, Shiba K, Iijima S
  • Organizer: Human Proteome Organization 11th Annual World Congress
  • Place of Presentation: Boston, USA.
  • Year and Date: 2012-09-10
• [Presentation] ワークショップI:基礎から臨床へ(1) 尿中エキソソーム成分から臨床検査診断薬開発へ (2012)
  • Author(s): 中山亜紀
  • Organizer: 第63回日本電気泳動学会総会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2012-08-20
• [Presentation] Constructive analysis of urinary proteins using cellulose acetate membrane electrophoresis for predicting renal damages (2012)
  • Author(s): Nakayama A, Odake J, Kimino M, Sakatsume M, Kasama K, Kanamori K, Shiba K
  • Organizer: American Association for Clinical Chemistry Annual meeting 2012
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2012-07-18
• [Presentation] Development of a highly sensitive enzyme-linked immunosorbent assay for urinary Tamm-Horsfall protein and its usefulness in diagnosing renal dysfunction (2012)
  • Author(s): Nakayama A, Kubota R, Nishimaki J, Odake J, Sakatsume M, Iijima S, Shiba K
  • Organizer: Association for Clinical Chemistry Annual meeting 2012
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2012-07-18
• [Presentation] 健常人尿中エキソソームタンパク質の基礎的解析 (2012)
  • Author(s): 中山 亜紀、香坂 亜沙美、平賀 香奈子、片山 映、鈴木 秀典、芝 紀代子
  • Organizer: 第22回生物試料分析学会年次学術集会
  • Place of Presentation: 九州市
  • Year and Date: 2012-03-11
• [Presentation] Constructive analysis of urinary proteins using cellulose acetate membrane electrophoresis for predicting renal damages (2012)
  • Author(s): Nakayama A, Odake J, Kimino M, Sakatsume M, Kasama K, Kanamori K, Shiba K.
  • Organizer: American Association for Clinical Chemistry Annual meeting 2012
  • Place of Presentation: Los Angeles, CA, USA
• [Presentation] Development of a highly sensitive enzyme-linked immunosorbent assay for urinary Tamm-Horsfall protein and its usefulness in diagnosing renal dysfunction (2012)
  • Author(s): Nakayama A, Kubota R, Nishimaki J, Odake J, Sakatsume M, Iijima S, Shiba K.
  • Organizer: American Association for Clinical Chemistry Annual meeting 2012
  • Place of Presentation: Los Angeles, USA
• [Presentation] ワークショップI：基礎から臨床へ（1） 尿中エキソソーム成分から臨床検査診断薬開発へ (2012)
  • Author(s): 中山亜紀
  • Organizer: 第63回日本電気泳動学会総会
  • Place of Presentation: 沖縄
  • Invited
• [Presentation] Identification of urinary exosomal proteins by two dimensional gel electrophoresis (2012)
  • Author(s): Nakayama A, Kubota R, Arai Y, Katayama A, Suzuki H, Shiba K, Iijima S.
  • Organizer: Human Proteome Organization 11th Annual World Congress
  • Place of Presentation: Boston, MA, USA
• [Presentation] Establishment of enzyme-linked immunosorbent assay for urinary Tamm-Horsfall protein (2012)
  • Author(s): Nakayama A, Kubota R, Sakatsume M, Iijima S, Kiyoko Shiba.
  • Organizer: 12th meeting of the Asian Society of Clinical pathology and Laboratory Medicine
  • Place of Presentation: 京都
• [Presentation] 健常人尿中エキソソームタンパク質の基礎的解析 (2012)
  • Author(s): 中山 亜紀、香坂 亜沙美、平賀 香奈子、片山 映、鈴木 秀典、芝 紀代子
  • Organizer: 第22回生物試料分析学会年次学術集会
  • Place of Presentation: 福岡
• [Presentation] 尿中エキソソームタンパク質の基礎的解析 (2012)
  • Author(s): 中山 亜紀、香坂 亜沙美、平賀 香奈子、片山 映、鈴木 秀典、芝 紀代子
  • Organizer: 第8回腎・泌尿器検査研究会学術集会
  • Place of Presentation: 東京
• [Presentation] 二次元電気泳動法による尿中エキソソームの解析 (2011)
  • Author(s): 香坂亜沙美、中山亜紀、山口委久江、戸塚実、芝紀代子
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2011-11-20
• [Presentation] セルロースアセテート膜電気泳動法により 9 分画に分離した尿細管障害患者のタンパク質解析 (2011)
  • Author(s): 大竹純矢、中山亜紀、坂爪実、成田一衛、笠間健嗣、芝紀代子
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2011-11-20
• [Presentation] 二次元電気泳動法を用いた健常人 尿中エキソソームタンパク質の解析 (2011)
  • Author(s): 中山亜紀、平賀香奈子、香坂亜沙美、片山映、鈴木秀典、芝紀代子
  • Organizer: 第62回日本電気泳動学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-11-12
• [Presentation] Cellulose acetate membrane electrophoresis coupled with sensitive colloidal silver staining for urinary approach for predicting the renal injury. Annual meeting 2011 (2011)
  • Author(s): Kubota R, Nakayama A, Odake J, Sakatsume M, Kasama T, Narita I, Sakai N, Kanamori K, Shiba K
  • Organizer: American Association for Clinical Chemistry
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2011-07-28
• [Presentation] Cellulose acetate membrane electrophoresis coupled with sensitive colloidal silver staining for urinary protein analysis: A rapid and simple approach for predicting the renal injury (2011)
  • Author(s): Ryo Kubota, Aki Nakayama (equal contribution), Junya Odake, Minoru Sakatsume, Takeshi Kasama, Ichiei Narita, Nobue Sakai, Kiyoko Kanamori, Kiyoko Shiba
  • Organizer: Annual meeting 2011, American Association for Clinical Chemistry
  • Place of Presentation: Atranta, GA, USA
• [Presentation] 二次元電気泳動法を用いた健常人 尿中エキソソームタンパク質の解析 (2011)
  • Author(s): 中山亜紀、平賀香奈子、香坂亜沙美、片山映、鈴木秀典、芝紀代子
  • Organizer: 第62 回日本電気泳動学会総会
  • Place of Presentation: 横浜
• [Presentation] 二次元電気泳動法による尿中エキソソームの解析 (2011)
  • Author(s): 香坂亜沙美、中山亜紀、山口委久江、戸塚実、芝紀代子
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山
• [Presentation] セルロースアセテート膜電気泳動法における腎疾患患者（糸球体障害型）の尿中アルブミン分子多様性の解析 (2011)
  • Author(s): 中山亜紀、大竹純矢、坂爪実、笠間健嗣、久保田亮、 成田一衛、芝紀代子
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山
• [Presentation] セルロースアセテート膜電気泳動法により9分画に分離した尿細管障害患者のタンパク質解析 (2011)
  • Author(s): 大竹純矢、中山亜紀、坂爪実、成田一衛、笠間健嗣、芝紀代子
  • Organizer: 第58回日本臨床検査医学会学術集会
  • Place of Presentation: 岡山
• [Presentation] 尿中エキソソームタンパク質の基礎的解析
  • Author(s): 中山 亜紀、香坂 亜沙美、平賀 香奈子、片山 映、鈴木 秀典、芝 紀代子
  • Organizer: 第8回腎・泌尿器検査研究会学術集会
  • Place of Presentation: 東京都