Regulation of NK cell activation by glycan

• Project/Area Number: 23790631
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Toho University
• Principal Investigator: HIGAI Koji 東邦大学, 薬学部, 准教授 (70297711)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: NK細胞 / 細胞傷害 / 糖鎖リガンド / シアル酸 / レクチン受容体 / 多糖類 / KLRs / NCRs

Research Abstract

In this study, we tried to clarify the regulation of activation of NK cells by polysaccharides. The reported glycanligands of NKRs and NCRs affected the cytotoxicity by NK cells and up-regulation of CD3zeta phosphorylation. These results suggested that the specific glycan of target cells affected the NK activation via NCR1 or NCR3 molecules

Research Products

• [Journal Article] Glycan Ligand Specificity of Killer Lectin Receptors (2012)
  • Author(s): K. Higai
  • Journal Title: YAKUGAKU ZASSHI Volume: 132 Issue: 6 Pages: 705-712
  • DOI: 10.1248/yakushi.132.705
  • NAID: 130001888685
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2012-06-01
• [Journal Article] Glycan ligand specificity of killer lectin receptors (2012)
  • Author(s): Higai K., Matsumoto K.
  • Journal Title: Yakugaku Zasshi Volume: 132(6) Pages: 705-12
  • NAID: 130001888685
  • URL: https://www.jstage.jst.go.jp/article/yakushi/132/6/132_705/_article
• [Journal Article] Unlike NKp30, natural cytotoxicity receptor NKp44 binds to multimeric alpha2,3-NeuNAc-containing N-glycans (2012)
  • Author(s): Ito K., Higai K., Shinoda C., Sakurai M., Yanai K., Azuma Y., Matsumoto K.
  • Journal Title: Biol. Pharm. Bull Volume: 35 (4) Pages: 1-7
  • URL: https://www.jstage.jst.go.jp/article/bpb/35/4/35_4_594/_article
  • Peer Reviewed
• [Journal Article] Unlike NKp30, natural cytotoxicity receptor NKp44 binds to multimeric alpha2,3-NeuNAc-containing N-glycans (2012)
  • Author(s): Ito K., Higai K., Shinoda C., Sakurai M., Yanai K., Azuma Y., Matsumoto K.
  • Journal Title: Biol. Pharm. Bull. Volume: 35 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Binding properties of CD94 to sulfated glycans and alpha2,3-NeuAc-containing glycoprotein and its mutagenesis analysis. (2011)
  • Author(s): Higai K., Matsumoto S.,Kimura M.,Imaizumi Y.,Yanai K., Azuma Y., Matsumoto K.
  • Journal Title: The Open Biotechnology Journal Volume: 5 Pages: 33-38
  • URL: http://www.benthamscience.com/open/tobiotj/articles/V005/33TOBIOTJ.pdf
  • Peer Reviewed
• [Journal Article] Binding properties of CD94 to sulfated glycans and alpha2,3-NeuAc-containing glycoprotein and its mutagenesis analysis (2011)
  • Author(s): Higai K., Itoh A., Imaizumi Y., Suzuki C., Xin X., Azuma Y. and Matsumoto K.
  • Journal Title: The Open Biotechnology Journal Volume: 5 Pages: 14-20
  • URL: http://www.benthamscience.com/open/tobiotj/articles/V005/14TOBIOTJ.pdf
  • Peer Reviewed
• [Journal Article] Binding of Natural cytotoxicity receptor NKp46 to sulfate- and alpha2,3-NeuAc-containing glycans and its mutagenesis (2011)
  • Author(s): Ito K., Higai K., Sakurai M., Shinoda C., Yanai K., Azuma Y. and Matsumoto K.
  • Journal Title: Biochem, Biophys. Res. Commun Volume: 406 (3) Pages: 377-82
  • URL: http://www.sciencedirect.com/science/article/pii/S0006291X11002427
  • Peer Reviewed
• [Journal Article] NKG2A and NKG2C bind to sulfated glycans and alpha2,3-NeuAc-containing glycoproteins (2011)
  • Author(s): Xin X, Higai K., Imaizumi Y., Suzuki C.,Ito K., Itoh A., Matsumoto S., Azuma Y.,and Matsumoto K.
  • Journal Title: Biol. Pharm. Bull Volume: 34(4) Pages: 480-485
  • URL: https://www.jstage.jst.go.jp/article/bpb/34/4/34_4_480/_article
  • Peer Reviewed
• [Journal Article] Binding affinities of NKG2D and CD94 to sialyl Lewis X-expressing N-glycans and heparin (2011)
  • Author(s): Higai K., Suzuki C., Imaizumi Y., Xin Xin, Azuma Y. and Matsumoto K
  • Journal Title: Biol. Pharm. Bull Volume: 34(1) Pages: 8-12
  • NAID: 130000402255
  • URL: https://www.jstage.jst.go.jp/article/bpb/34/1/34_1_8/_article
  • Peer Reviewed
• [Journal Article] Binding affinities of NKG2D and CD94 to sialyl Lewis X-expressing N-glycans and heparin (2011)
  • Author(s): Higai K., Suzuki C., Imaizumi Y., Xin Xin, Azuma Y.,Matsumoto K
  • Journal Title: Biol. Pharm. Bull. Volume: 34 Pages: 8-12
  • NAID: 130000402255
  • Peer Reviewed
• [Journal Article] NKG2A and NKG2C bind to sulfated glycans and alpha2,3-NeuAc-containing glycoproteins (2011)
  • Author(s): Xin X, Higai K., Imaizumi Y., Suzuki C.,Ito K., Itoh A., Matsumoto S., Azuma Y., Matsumoto K
  • Journal Title: Biol. Pharm. Bull. Volume: 34 Pages: 480-485
  • Peer Reviewed
• [Journal Article] Binding of Natural cytotoxicity receptor NKp46 to sulfate- and alpha2,3-NeuAc-containing glycans and its mutagenesis (2011)
  • Author(s): Ito K., Higai K., Sakurai M., Shinoda C., Yanai K., Azuma Y., Matsumoto K.
  • Journal Title: Biochem Biophys. Res. Commun. Volume: 406 Pages: 377-382
  • Peer Reviewed
• [Journal Article] Binding properties of CD94 to sulfated glycans and akpha2,3-NeuAc-containing glycoprotein and its mutagenesis analysis (2011)
  • Author(s): Higai K., Itoh A., Imaizumi Y., Suzuki C., Xin X., Azuma Y. Matsumoto K
  • Journal Title: The Open Biotechnology Journal Volume: 5 Pages: 14-20
  • Peer Reviewed
• [Journal Article] Binding properties of CD94 to sulfated glycans and alpha2,3-NeuAc-containing glycoprotein and its mutagenesis analysis (2011)
  • Author(s): Higai K., Matsumoto S.,Kimura M.,Imaizumi Y.,Yanai K., Azuma Y., Matsumoto K.
  • Journal Title: The Open Biotechnology Journal Volume: 5 Pages: 33-38
  • Peer Reviewed
• [Presentation] NCRsの糖鎖リガンドの解析 (2012)
  • Author(s): 伊藤 健一郎、篠田 千尋、桧貝 孝慈、松本 宏治郎
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡マリンメッセ
  • Year and Date: 2012-12-16
• [Presentation] KillerLectin-likeReceptors(KLRs) の 糖 鎖 リガン ド特異性 (2012)
  • Author(s): 今泉 雄三、小松 由佳、桧貝 孝慈、東 祐太郎、松本 宏治郎
  • Organizer: 第133回日本薬学会年会
  • Place of Presentation: パフィシコ横浜
  • Year and Date: 2012-03-29
• [Presentation] Naturalcytotoxicityreceptors(NCRs) の 糖鎖リガンド特異性 (2012)
  • Author(s): 伊藤 健一郎、高橋 真美子、桧貝 孝慈、東 祐太郎、吉田 直弘、松本 宏治郎
  • Organizer: 第133回日本薬学会年会
  • Place of Presentation: パフィシコ横浜
  • Year and Date: 2012-03-29
• [Presentation] キラーレクチン様受容体の糖鎖リガンドの解析 (2011)
  • Author(s): 桧貝 孝慈、今泉 雄三、Xin Xin 、松本 早代 、伊藤 あゆみ 、木村 恵、柳内 和幸 、東 祐太郎 、松本 宏治郎
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2011-09-24
• [Presentation] CRsの糖鎖リガンドの解析 (2011)
  • Author(s): 篠田 千尋、桧貝 孝慈、伊藤 健一郎、櫻井 瑞葉、柳内 和幸、東 祐太郎、松本 宏治郎
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2011-09-24
• [Presentation] NCRsの糖鎖リガンドの解析 (2011)
  • Author(s): 篠田 千尋、桧貝 孝慈、伊藤 健一郎、櫻井 瑞葉、柳内 和幸、東 祐太郎、松本 宏治郎
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都)
• [Presentation] キラーレクチン様受容体の糖鎖リガンドの解析 (2011)
  • Author(s): 桧貝 孝慈、今泉 雄三 、Xin Xin 、松本 早代 、伊藤 あゆみ 、木村 恵、柳内 和幸 、東 祐太郎 、松本 宏治郎
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都)
• [Presentation] NCRsの糖鎖リガンドの解析
  • Author(s): 伊藤 健一郎
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] NCRsの糖鎖リガンドの解析
  • Author(s): 伊藤 健一郎
  • Organizer: 第133回日本薬学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] KillerLectin-likeReceptors(KLRs)の糖鎖 リガン ド特異性
  • Author(s): 今泉 雄三
  • Organizer: 第133回日本薬学会年会
  • Place of Presentation: パシフィコ横浜