• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Involvement of ASIC3 in rat model of isoproterenol induced ischemic cardiac pain

Research Project

Project/Area Number 23790640
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Pain science
Research InstitutionKanazawa University

Principal Investigator

YAMAGUCHI TAKESHI  金沢大学, 医学系, 助教 (60532182)

Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords心虚血 / 心臓痛 / イソプロテレノール / 行動解析 / 心電図 / 虚血性心疾患 / ASIC / 炎症 / Amiloride / 免疫組織化学 / 薬物モデル / モルヒネ / 侵害刺激 / 痛み / ASIC3 / 心臓知覚神経 / c-fos / ECG
Outline of Final Research Achievements

【Aim】We characterized Isoproterenol(Iso)-induced myocardial ischemia in rat to clarify the involvement of acid-sensing ion channel (ASIC) 3 in ischemic cardiac pain.【Methods】Male SD rats were injected with 50 mg/kg of Iso subcutaneously. The behavioral changes and c-Fos expression in intra-spinal cord neurons after Iso-injection were analyzed. Furthermore, effects of morphine (Mor, 1 mg/kg) and ASICs antagonist amiloride (Ami, 30 mg/kg) on behavioral changes and c-fos expression were examined.【Results】Rats showed characteristic behaviors after Iso-injection. The rats lay on their side or back with their head extended. Iso group expressed more c-Fos in intra-spinal cord neurons compared to control group significantly. Mor and Ami significantly reduced the characteristic behaviors and the c-Fos expression respectively.【Conclusion】These results suggest that ASICs were involved in Iso-induced ischemic cardiac pain.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (7 results)

All 2015 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (6 results)

  • [Journal Article] Immuno-histochemistry and three-dimensional architecture of the intermediate filaments in Purkinje cells in mammalian hearts.2014

    • Author(s)
      Yoshimura A, Yamaguchi T, Kawazato H, Takahashi N, Shimada T.
    • Journal Title

      Med Mol Morphol

      Volume: 47 Issue: 4 Pages: 233-239

    • DOI

      10.1007/s00795-014-0069-9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Involvement of ASICs in isoproterenol induced ischemic cardiac pain rat model2015

    • Author(s)
      Takeshi Yamaguchi, Kiyomi Hori, Yu Kozakai, Shuangqin Yi, Noriyuki Ozaki
    • Organizer
      第120回日本解剖学会・第92回日本生理学会 合同学術集会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Isoproterenol投与による心臓痛モデルラットにおける心電図について2014

    • Author(s)
      山口豪、堀紀代美、小酒井友、白石昌武、中村恒夫、易勤、尾﨑紀之
    • Organizer
      第29回日本心電学会・第31回日本不整脈学会 合同学術集会
    • Place of Presentation
      ザ・プリンスパークタワー東京、東京プリンスホテル
    • Year and Date
      2014-07-22 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Isoproterenol投与による虚血性心臓痛におけるASICsの関与の検討2014

    • Author(s)
      山口豪、堀紀代美、小酒井友、Aye Aye Mon、中村恒夫、白石昌武、易勤、尾﨑紀之
    • Organizer
      第36回日本疼痛学会
    • Place of Presentation
      KKRホテル大阪
    • Year and Date
      2014-06-20 – 2014-06-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 行動ならびにc-Fos発現の変化を指標としたIsoproterenol投与による虚血性心臓痛モデルの有用性の検討2013

    • Author(s)
      山口豪,堀紀代美,小酒井友,白石昌武,中村恒夫,易勤,尾﨑紀之
    • Organizer
      第73回日本解剖学会中部支部学術集会
    • Place of Presentation
      山梨大学(甲府市)
    • Related Report
      2013 Research-status Report
  • [Presentation] イソプロテレノール投与による虚血性心筋傷害疼痛モデルの有用性の検討2013

    • Author(s)
      山口豪,堀紀代美,小酒井友,中村恒夫,白石昌武,易勤,尾﨑紀之
    • Organizer
      第12回コ・メディカル形態機能学会
    • Place of Presentation
      広島国際大学(呉市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Isoproterenol虚血性心筋傷害モデルにおける行動学的ならびに脊髄後角ニューロンにおけるc-fos発現の変化

    • Author(s)
      山口豪
    • Organizer
      第118回 日本解剖学会全国学術集会
    • Place of Presentation
      サンポートホール高松・かがわ国際会議場(香川県高松市)
    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi