Involvement of ASIC3 in rat model of isoproterenol induced ischemic cardiac pain
| Project/Area Number |
23790640
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pain science
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 心虚血 / 心臓痛 / イソプロテレノール / 行動解析 / 心電図 / 虚血性心疾患 / ASIC / 炎症 / Amiloride / 免疫組織化学 / 薬物モデル / モルヒネ / 侵害刺激 / 痛み / ASIC3 / 心臓知覚神経 / c-fos / ECG |
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| Outline of Final Research Achievements |
【Aim】We characterized Isoproterenol(Iso)-induced myocardial ischemia in rat to clarify the involvement of acid-sensing ion channel (ASIC) 3 in ischemic cardiac pain.【Methods】Male SD rats were injected with 50 mg/kg of Iso subcutaneously. The behavioral changes and c-Fos expression in intra-spinal cord neurons after Iso-injection were analyzed. Furthermore, effects of morphine (Mor, 1 mg/kg) and ASICs antagonist amiloride (Ami, 30 mg/kg) on behavioral changes and c-fos expression were examined.【Results】Rats showed characteristic behaviors after Iso-injection. The rats lay on their side or back with their head extended. Iso group expressed more c-Fos in intra-spinal cord neurons compared to control group significantly. Mor and Ami significantly reduced the characteristic behaviors and the c-Fos expression respectively.【Conclusion】These results suggest that ASICs were involved in Iso-induced ischemic cardiac pain.
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Report
(5 results)
Research Products
(7 results)
