In vivo patch-clamp recording analysis of a pruritogen-induced itching on synaptic transmission in spinal dorsal horn

• Project/Area Number: 23790652
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pain science
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: UTA Daisuke 生理学研究所, 生体情報研究系, 特任助教 (70598416)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 痒み / in vivo パッチクランプ / シナプス / 脊髄後角 / セロトニン / EPSC / 膠様質細胞 / c-fos / in vivo patch clamp / itch / pain / spinal cord / シナプス伝達 / 痛み / 脊髄

Research Abstract

The mechanism for the itch transmission in the spinal dorsal horn remains unclear. The present study was designed to analyze pruritic synaptic responses evoked in spinal dorsal horn neurons of rodents by using an in vivo whole-cell patch-clamp recording technique. The present main finding is that subpopulation of spinal dorsal horn neurons are responsible for pruritic cutaneous serotonin stimulation.

Research Products

• [Journal Article] Identification of 5-HT receptor subtypes enhancing inhibitory transmission in the rat spinal dorsal horn in vitro (2012)
  • Author(s): Xie DJ, Uta D, Feng PY, Wakita M, Shin MC, Furue H and Yoshimura M
  • Journal Title: Mol Pain Volume: 8(1) Pages: 58-58
  • DOI: 10.1186/1744-8069-8-58
  • Peer Reviewed
• [Journal Article] Morphological analyses of excitatory effect of TRPA1 agonists on synaptic transmission in spinal dorsal neurons (2012)
  • Author(s): Uta D, Furue H, Mizuguchi-Takase H, Imoto K and Yoshimura M
  • Journal Title: The Journal of Functional Diagnosis of the Spinal Cord Volume: 34(1) Pages: 58-64
  • Peer Reviewed
• [Journal Article] Morphological analyses of excitatory effect of TRPA1 agonists on synaptic transmission in spinal dorsal neurons. (2012)
  • Author(s): Uta D, Furue H, Mizuguchi-Takase H, Imoto K and Yoshimura M.
  • Journal Title: The Journal of Functional Diagnosis of the Spinal Cord Volume: 34 Pages: 58-64
  • Peer Reviewed
• [Journal Article] Rapid S-nitrosylation of actin by NO-generating donors and in inflammatory pain model mice. (2011)
  • Author(s): Lu J, Katano T, Uta D, Furue H and ItoS
  • Journal Title: Mol Pain7 Volume: 101(1-13) Pages: 101-101
  • DOI: 10.1186/1744-8069-7-101
  • Peer Reviewed
• [Journal Article] Activation of TRPA1 on excitatory synaptic transmission in sbstantia gelatinosa of the adult rat spinal cord (2011)
  • Author(s): Uta D, Furue H, Imoto K and Yoshimura M
  • Journal Title: The Journal of Functional Diagnosis of the Spinal Cord Volume: 33(1) Pages: 24-29
  • Peer Reviewed
• [Journal Article] Plastic changes in spinal excitatory synaptic transmission in cancer-bearing mice (2011)
  • Author(s): Yanagisawa Y, Uta D, Kawamata T, Furue H, Iwamoto Y, Imoto K and Yoshimura M
  • Journal Title: The Journal of Functional Diagnosis of the Spinal Cord Volume: 33(1) Pages: 8-17
  • NAID: 40019198113
  • Peer Reviewed
• [Journal Article] Activation of TRPA1 on excitatory synaptic transmission in sbstantia gelatinosa of the adult rat spinal cord. (2011)
  • Author(s): Uta D, Furue H, Imoto K and Yoshimura M.
  • Journal Title: The Journal of Functional Diagnosis of the Spinal Cord Volume: 33 Pages: 24-29
  • Peer Reviewed
• [Journal Article] Plastic changes in spinal excitatory synaptic transmission in cancer-bearing mice. (2011)
  • Author(s): Yanagisawa Y, Uta D, Kawamata T, Furue H, Iwamoto Y, Imoto K and Yoshimura M.
  • Journal Title: The Journal of Functional Diagnosis of the Spinal Cord Volume: 33 Pages: 8-17
  • NAID: 40019198113
  • Peer Reviewed
• [Presentation] Firing pattern and morphological analysis of substantia gelatinosa neurons receiving TRPA1-expressing afferents in rat spinal dorsal horn (2013)
  • Author(s): Uta D, Imoto K and Furue H
  • Organizer: The 90th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2013-03-29
• [Presentation] In vivo patch clamp analysis of itch transmission in the rat spinal dorsal horn (2013)
  • Author(s): Uta D
  • Organizer: Symposium on Behavioral Molecular Neuroscience 2013
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2013-03-26
• [Presentation] In vivo spinal synaptic responses and scratching behaviors evoked bycutaneous 5-HT application (2013)
  • Author(s): Uta D, Furue H and Imoto K
  • Organizer: 2nd Joint conference of BRI and NIPS
  • Place of Presentation: 新潟大学脳研究所附属統合脳機能研究センター(新潟県)
  • Year and Date: 2013-02-18
• [Presentation] 痒み物質の皮膚投与に より誘起される行動学的解析とin vivo パッチクランプ記録法を用いた脊髄後 角におけるシナプス応答の解析 (2012)
  • Author(s): 歌 大介、安東嗣修、倉石 泰、井本敬二、古江秀昌
  • Organizer: 第59回中部日本生理学会
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター(愛知県)
  • Year and Date: 2012-11-16
• [Presentation] The relationship between CCL-1 and neuron/glia in the neuropathic pain model (2012)
  • Author(s): Akimoto N, Honda K, Uta D, Furue H, Imoto K, Takano Y and Noda M
  • Organizer: The 42th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2012-10-17
• [Presentation] In vivo puritic synaptic responses elicited by cutaneous 5-HT application in the superficial spinal dorsal horn of adult rats (2012)
  • Author(s): Uta D, Gotoh Y, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: The 42th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2012-10-15
• [Presentation] 局所 麻酔薬光学異性体の分離遮断能とTRP チ ャネルをターゲットにした新たな鎮痛 薬の可能性について., (2012)
  • Author(s): 歌 大介、古江秀昌、井本敬二
  • Organizer: 第3回名古屋大学医学研究科・生理学研究所合同シンポジウム
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター(愛知県)
  • Year and Date: 2012-09-29
• [Presentation] In vivo spinal synaptic responses and scratching behaviors evoked by cutaneous serotonin application (2012)
  • Author(s): Uta D, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: The 35rd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2012-09-19
• [Presentation] In vivo spinal synaptic responses and scratching behaviors evoked by cutaneous 5-HT application (2012)
  • Author(s): Uta D, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: NIPS International Workshop 2012 Central Neuroplasticity in Sensory-Emotional Link
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター(愛知県)
  • Year and Date: 2012-09-14
• [Presentation] In vivo patch-clamp analysis of local anesthetic actions on rat spinal nociceptive synaptic transmission (2012)
  • Author(s): Uta D, Imoto K and Furue H
  • Organizer: 第34回日本疼痛学会
  • Place of Presentation: 熊本市国際交流会館(熊本県)
  • Year and Date: 2012-07-21
• [Presentation] Excitatory effect of a pruritogen serotonin on synaptic transmission in adult rat spinal dorsal horn neurons in vivo (2012)
  • Author(s): Uta D, Gotoh Y, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: The 89th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 長野県松本文化会館、松本市総合体育館、信州大学松本キャンパス(長野県)
  • Year and Date: 2012-03-29
• [Presentation] Plastic changes in nociceptive excitatory synaptic transmission induced in bone cancer model mice, and selective antinociceptive actions of local anesthetics and TRPA1 channel agonist (2012)
  • Author(s): Uta D, Yanagisawa Y, Furue H and Imoto K
  • Organizer: 42nd NIPS International Symposium - NIPS and BRI Niigata University Joint Symposium - " Advanced Research Areas for the Future Breakthrough in Neuroscience"
  • Place of Presentation: 自然科学研究機構生理学研究所(愛知県)
  • Year and Date: 2012-03-06
• [Presentation] Plastic changes in nociceptive excitatory synaptic transmission induced of bone cancer model mice, and selective antinociceptive actions of TRPA1 channel agonists and local anesthetics (2012)
  • Author(s): Uta D
  • Organizer: 第4回NAGOYA グローバルリトリート
  • Place of Presentation: あいち健康プラザ(愛知県)
  • Year and Date: 2012-02-24
• [Presentation] TRPA1 発現線維が入力する脊髄後角細胞の形態学的解析 (2012)
  • Author(s): 歌 大介、古江秀昌、吉村 恵、井本敬二
  • Organizer: 第34回脊髄機能診断研究会
  • Place of Presentation: 東京商工会議所(東京都)
  • Year and Date: 2012-02-04
• [Presentation] TRPA1発現線維が入力する脊髄後角細胞の形態学的解析。 (2012)
  • Author(s): 歌 大介、古江 秀昌、吉村 恵、井本 敬二.
  • Organizer: 第34回脊髄機能診断研究会
  • Place of Presentation: 東京商工会議所（東京都）
• [Presentation] Plastic changes in nociceptive excitatory synaptic transmission induced of bone cancer model mice, and selective antinociceptive actions of TRPA1 channel agonists and local anesthetics. (2012)
  • Author(s): Uta D.
  • Organizer: 第4回NAGOYAグローバルリトリート
  • Place of Presentation: あいち健康プラザ（愛知県）
• [Presentation] Plastic changes in nociceptive excitatory synaptic transmission induced in bone cancer model mice, and selective antinociceptive actions of local anesthetics and TRPA1 channel agonist. (2012)
  • Author(s): Uta D, Yanagisawa Y, Furue H and Imoto K.
  • Organizer: 42nd NIPS International Symposium - NIPS and BRI Niigata University Joint Symposium - "Advanced Research Areas for the Future Breakthrough in Neuroscience"
  • Place of Presentation: 自然科学研究機構生理学研究所（愛知県）
• [Presentation] Excitatory effect of a pruritogen serotonin on synaptic transmission in adult rat spinal dorsal horn neurons in vivo. (2012)
  • Author(s): Uta D, Gotoh Y, Andoh T, Kuraishi Y, Imoto K and Furue H．
  • Organizer: The 89th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 長野県松本文化会館、松本市総合体育館、信州大学松本キャンパス（長野県）
• [Presentation] 光学異性体の痛覚伝達に対する選択的抑制効果 (2011)
  • Author(s): 歌 大介、井本敬二、古江秀昌
  • Organizer: 「痛みの病態生理と神経・分子 機構」平成23 年度生理研研究会
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター(愛知県)
  • Year and Date: 2011-12-21
• [Presentation] In vivo patch-clamp analysis of pruriceptive and nociceptive synaptic transmission in the adult rat spinal dorsal horn (2011)
  • Author(s): Uta D, Gotoh Y, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: The 41th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Washington D.C. (USA)
  • Year and Date: 2011-11-16
• [Presentation] Sensitization of TRPV1 receptors expressed at the central end of C afferents following inflammation (2011)
  • Author(s): Yoshimura M, Xie D, Uta D, Hachisuka J and Furue H
  • Organizer: The 41th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Washington D.C. (USA)
  • Year and Date: 2011-11-14
• [Presentation] Naftoidil の脊髄膠様質細胞に対する作用の電気生理学的解析 (2011)
  • Author(s): 謝 篤傑、歌 大介、服部 剛、笠原憲一、吉村 恵
  • Organizer: 第62回西日本生理学会
  • Place of Presentation: 佐賀大学医学部臨床大講堂(佐賀県)
  • Year and Date: 2011-10-15
• [Presentation] In vivo パッチクランプ法を用いた痛覚シナプス伝達の解析 (2011)
  • Author(s): 歌 大介
  • Organizer: 第7回若手の会 セルセンサー研究の未来予想図
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター(愛知県)
  • Year and Date: 2011-09-24
• [Presentation] In vivo patch-clamp recording analysis of a pruritogen serotonin-induced itching on synaptic transmission in substantia gelatinosa neurons (2011)
  • Author(s): Uta D, Gotoh Y, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: The 34rd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-17
• [Presentation] TRPA1発現線維が入力する脊髄膠様質細胞の形態学的特徴。 (2011)
  • Author(s): 歌 大介、井本敬二、古江秀昌.
  • Organizer: 第13回ブレインサイエンス研究会
  • Place of Presentation: アソシエート（熊本県）
• [Presentation] In vivo痛覚シナプス伝達の異常と新規鎮痛法の開発。ガン性疼痛モデル興奮性シナプス伝達の可塑的変化とTRP受容体活性化による痛覚伝達の選択的な遮断。 (2011)
  • Author(s): 歌 大介、柳澤 義和、古江 秀昌、井本敬二.
  • Organizer: 第2回名古屋大学医学研究科・生理学研究所合同シンポジウム
  • Place of Presentation: 名古屋大学医学部付属病院（愛知県）
• [Presentation] Functional role of the TRPV1 receptor expressed at the terminals of primary afferent C fibers in the spinal dorsal horn. (2011)
  • Author(s): Yoshimura M, Duxie Xie, Uta D.
  • Organizer: The 34rd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] In vivo patch-clamp recording analysis of a pruritogen serotonin-induced itching on synaptic transmission in substantia gelatinosa neurons. (2011)
  • Author(s): Uta D, Gotoh Y, Andoh T, Kuraishi Y, Imoto K and Furue H.
  • Organizer: The 34rd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] In vivoパッチクランプ法を用いた痛覚シナプス伝達の解析。 (2011)
  • Author(s): 歌 大介.
  • Organizer: 第7回若手の会 セルセンサー研究の未来予想図(招待講演)
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター（愛知県）
• [Presentation] TRPチャネルを利用した選択的な痛覚伝達抑制。 (2011)
  • Author(s): 歌 大介.
  • Organizer: 生理学研究所昼食セミナー
  • Place of Presentation: 自然科学研究機構生理学研究所（愛知県）
• [Presentation] Sensitization of TRPV1 receptors expressed at the central end of C afferents following inflammation. (2011)
  • Author(s): Yoshimura M, Xie D, Uta D, Hachisuka J and Furue H.
  • Organizer: The 41th Annual Meeting of the Society for Neuroscience.
  • Place of Presentation: Washington D.C. (USA)
• [Presentation] In vivo patch-clamp analysis of pruriceptive and nociceptive synaptic transmission in the adult rat spinal dorsal horn. (2011)
  • Author(s): Uta D, Gotoh Y, Andoh T, Kuraishi Y, Imoto K and Furue H.
  • Organizer: The 41th Annual Meeting of the Society for Neuroscience.
  • Place of Presentation: Washington D.C. (USA)
• [Presentation] Naftoidilの脊髄膠様質細胞に対する作用の電気生理学的解析。 (2011)
  • Author(s): 謝 篤傑、歌 大介、服部 剛、笠原 憲一、吉村 恵.
  • Organizer: 第62回西日本生理学会
  • Place of Presentation: 佐賀大学医学部臨床大講堂（佐賀県）
• [Presentation] 光学異性体の痛覚伝達に対する選択的抑制効果。 (2011)
  • Author(s): 歌 大介、井本 敬二、古江 秀昌.
  • Organizer: 「痛みの病態生理と神経・分子機構」平成23年度生理研研究会
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター（愛知県）
• [Presentation] In vivoパッチクランプ法を用いた痒みの研究
  • Author(s): 歌 大介、井本敬二、古江秀昌
  • Organizer: 第14回ブレインサイエンス研究会
  • Place of Presentation: 霧島国際ホテル（鹿児島県）
• [Presentation] CCL-1の疼痛発現との連関、および神経細胞-グリア細胞連関の解明
  • Author(s): 秋元 望、本多健治、歌 大介、古江秀昌、井本敬二、高野行夫、野田百美
  • Organizer: 第14回ブレインサイエンス研究会
  • Place of Presentation: 霧島国際ホテル（鹿児島県）
• [Presentation] In vivo patch-clamp analysis of local anesthetic actions on rat spinal nociceptive synaptic transmission
  • Author(s): Uta D, Imoto K and Furue H
  • Organizer: 第34回日本疼痛学会
  • Place of Presentation: 熊本市国際交流会館（熊本県）
• [Presentation] In vivo spinal synaptic responses and scratching behaviors evoked by cutaneous 5-HT application
  • Author(s): Uta D, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: NIPS International Workshop 2012 Central Neuroplasticity in Sensory-Emotional Link
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター（愛知県）
• [Presentation] In vivo spinal synaptic responses and scratching behaviors evoked by cutaneous serotonin application
  • Author(s): Uta D, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: The 35rd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] 局所麻酔薬光学異性体の分離遮断能とTRPチャネルをターゲットにした新たな鎮痛薬の可能性について
  • Author(s): 歌 大介、古江秀昌、井本敬二
  • Organizer: 第3回名古屋大学医学研究科・生理学研究所合同シンポジウム
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター（愛知県）
• [Presentation] In vivo puritic synaptic responses elicited by cutaneous 5-HT application in the superficial spinal dorsal horn of adult rats
  • Author(s): Uta D, Gotoh Y, Andoh T, Kuraishi Y, Imoto K and Furue H
  • Organizer: The 42th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Ernest N. Morial Convention Center（New Orleans, USA）
• [Presentation] The relationship between CCL-1 and neuron/glia in the neuropathic pain model
  • Author(s): Akimoto N, Honda K, Uta D, Furue H, Imoto K, Takano Y and Noda M
  • Organizer: The 42th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Ernest N. Morial Convention Center（New Orleans, USA）
• [Presentation] 痒み物質の皮膚塗布により誘起される行動学的解析とin vivoパッチクランプ記録法を用いた脊髄後角におけるシナプス応答の解析
  • Author(s): 歌 大介、安東嗣修、倉石 泰、井本敬二、古江秀昌
  • Organizer: 第59回中部日本生理学会
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター（愛知県）
• [Presentation] 局所麻酔薬レボブピバカインのrNav1.8 チャネルに対する作用
  • Author(s): 川津領一、井本敬二、歌 大介、岡本孝史、増井邦晴、田矢廣司、山村睦朗、池田雅弘、古江秀昌
  • Organizer: 「痛み研究の新たな展開」平成24年度生理研研究会
  • Place of Presentation: 岡崎統合バイオサイエンスセンター（愛知県）
• [Presentation] In vivo spinal synaptic responses and scratching behaviors evoked bycutaneous 5-HT application
  • Author(s): Uta D, Furue H and Imoto K
  • Organizer: 2nd Joint conference of BRI and NIPS
  • Place of Presentation: 新潟大学脳研究所附属統合脳機能研究センター（新潟県）
• [Presentation] In vivo patch clamp analysis of itch transmission in the rat spinal dorsal horn
  • Author(s): Uta D
  • Organizer: Symposium on Behavioral Molecular Neuroscience 2013
  • Place of Presentation: 東京国際フォーラム（東京都）
  • Invited
• [Presentation] Inhibitory action of levobupivacaine on TTX resistant（NaV1.8）sodium channel
  • Author(s): Kawatsu R, Imoto K, Uta D, Okamoto T, Andoh T, Masui K, Taya K, Yamamura Y, Ikeda M and Furue H
  • Organizer: The 90th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: タワーホール船堀（東京都）
• [Presentation] Firing pattern and morphological analysis of substantia gelatinosa neurons receiving TRPA1-expressing afferents in rat spinal dorsal horn
  • Author(s): Uta D, Imoto K and Furue H
  • Organizer: The 90th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: タワーホール船堀（東京都）
• [Presentation] Full visualization of single trigeminal ganglion neuron
  • Author(s): Tonomura S, Meir I, Ebara S, Kuroda D, Uta D, Furue H, Furuta T, Bagdasarian K, Ahissar E and Kumamoto K
  • Organizer: The 90th Annual Meeting of the Japanese Association of Anatomy
  • Place of Presentation: サンポートホール高松かがわ国際会議場（香川県）
• [Remarks]
  • URL: http://www.nips.ac.jp/huinfo/