| Project/Area Number |
23790680
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hygiene
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
SUZUKI Takehiro 独立行政法人国立環境研究所, 環境健康研究センター, 主任研究員 (60425494)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ヒ素 / エピジェネティクス / ヒストン修飾 / P16INK4a |
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| Research Abstract |
In this study, we investigated the mechanism involved in arsenic-induced carcinogenesis focusing on epigenetic regulations of the tumor suppressor gene p16^<1NK4a> in human urothelial cell lines. The results showed that arsenic exposure induced a significant decrease in expression of p16^<1NK4a> associated with an increase in the level of trimethylated histone H3 lysine 27 (H3K27), a transcription-suppressive histone modification, in the promoter region. The results also showed that arsenic exposure induced a significant increase in expression of H3K27 histone methyltransferase EZH2. These results suggested that the decreased expression of p16^<1NK4a> through histone modification is associated with arsenic-induced carcinogenesis in human urothelial cell lines.
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