| Project/Area Number |
23790681
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hygiene
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| Research Institution | National Institute of Occupational Safety and Health, Japan |
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Principal Investigator |
YANAGIBA Yukie 独立行政法人労働安全衛生総合研究所, 健康障害予防研究, 研究員 (90467283)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | CYP2E1 / DMAC / 肝障害 / ジメチルアセトアミド / 肝機能障害 / 有機溶剤 / 酸化ストレス / 個体差要因 / 炎症 / 毒性メカニズム |
|---|
| Research Abstract |
DMAC is widely used in pharmaceutical and industrial chemical compounds. The cyp2e1+/+and cyp2e1-/- mice were exposed to inhalation of DMAC or vehicle control (10, 25, 50 and 250ppm) for 14 days., In cyp2e1+/+ and cyp2e1-/-mice, plasma AST and ALT activities were significantly increased at 50 and 250ppm of DMAC. The histological changes were observed in both genotypes. In addition, focal necrosis and fatty degeneration was also observed in the cyp2e1-/- at the high concentration exposure. cyp2e1-/- were detected in the exposed group for NMAC and DMAC in mice urine. Further, NMAC and DMAC was high concentration as compared to cyp2e1+/+ mice. cyp2e1+/+ were detected in the exposed group for acetamide in mice urine. Hence, most of the DMAC metabolism takes place via CYP2E1, but it is indicated that there is a possibility that other pathways also exist.
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