Elucidation of transcriptional regulation mechanism of ABO gene based on chromatin remodeling.

• Project/Area Number: 23790723
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: Gunma University
• Principal Investigator: SANO Rie 群馬大学, 大学院・医学系研究科, 助教 (70455955)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ABO / 転写調節機構 / ABO式血液型 / 転写調節 / ルシフェラーゼアッセイ / ABO式血液型変異型Bm型

Research Abstract

An erythroid cell-specific regulatory element has been identified in the first intron of ABO using luciferase reporter assays with K562 cells. The erythroid cell-specific regulatory activity of the element was dependent upon GATA-1 binding. In addition, partial deletion of intron 1 including the element was observed in genomic DNAs obtained from 111 Bm and ABm individuals.

Research Products

• [Journal Article] Mutation of the GATA site in the erythroid cell-specific regulatory element of the ABO gene in a Bm subgroup individual. (2013)
  • Author(s): Nakajima T, Sano R, Takahashi Y, Kubo R, Takahashi K, Kominato Y, Tsukada J, Takeshita H, Yasuda T, Uchikawa M, Isa K, Ogasawara K.
  • Journal Title: Transfusion. Volume: in press
• [Journal Article] Six VKORC1 polymorphims for Asians and its survey on ethnic wide-ranging populations. (2013)
  • Author(s): Fujihara J, Yasuda T, Kimura-Kataoka K, Sano R, Nakajima T, Kominato Y, Takeshita H.
  • Journal Title: DNA Pol. Volume: in press
• [Journal Article] Expression of ABO blood-group genes is dependent upon an erythroid cell-specific regulatory element that is deleted in persons with the Bm phenotype (2012)
  • Author(s): Sano R, Nakajima T, Takahashi K, Kubo R, Kominato Y, Tsukada J, Takeshita H, Yasuda T, Ito K, Maruhashi T, Yokohama A, Isa K, Ogasawara K, Uchikawa M
  • Journal Title: Blood Volume: 119 Pages: 5301-10
• [Journal Article] Genetic and expression analysis of SNPs in the human deoxyribonuclease II: SNPs in the promoter region reduce its in vivo activity through decreased promoter activity (2012)
  • Author(s): Kimura-Kataoka K, Yasuda T, Fujihara J, Toga T, Ono R, Otsuka Y, Ueki M, Iida R, Sano R, Nakajima T, Kominato Y, Kato H, Takeshita H
  • Journal Title: Electrophoresis Volume: 33 Pages: 2852-58
  • Peer Reviewed
• [Journal Article] ABO式血液型遺伝子3'領域は遺伝子発現の抑制に関与する (2012)
  • Author(s): 佐野利恵,中島たみ子,高橋圭子,窪理英子,矢澤伸,小湊慶彦
  • Journal Title: DNA多型 Volume: 20 Pages: 241-245
  • Peer Reviewed
• [Journal Article] Expression of ABO blood-group genes is dependent upon an erythroid cell-specific regulatory element that is deleted in persons with the Bm phenotype. (2012)
  • Author(s): Sano R, Nakajima T, Takahashi K, Kubo R, Kominato Y, Tsukada J, Takeshita H, Yasuda T, Ito K, Maruhashi T, Yokohama A, Isa K, Ogasawara K, Uchikawa M.
  • Journal Title: Blood. Volume: 119 Pages: 5301-10
• [Journal Article] Genetic and expression analysis of SNPs in the human deoxyribonuclease II: SNPs in the promoter region reduce its in vivo activity through decreased promoter activity. (2012)
  • Author(s): Kimura-Kataoka K, Yasuda T, Fujihara J, Toga T, Ono R, Otsuka Y, Ueki M, Iida R, Sano R, Nakajima T, Kominato Y, Kato H, Takeshita H.
  • Journal Title: Electrophoresis. Volume: 33 Pages: 2852-58
• [Journal Article] Use of postmortem computed tomography to reveal acute subdural hematoma in a severely decomposed body with advanced skeletonization. (2012)
  • Author(s): Sano R, Hirasawa S, Awata S, Kobayashi S, Shimada T, Takei H, Takahashi Y, Kominato Y.
  • Journal Title: Leg Med Volume: 15 Pages: 32-4
• [Journal Article] The 3' flanking region of the human ABO histo-blood group gene is involved in negative regulation of gene expression (2011)
  • Author(s): Sano R, Nakajima T, Takahashi K, Kubo R, Yazawa S, Kominato Y
  • Journal Title: Leg Med Volume: 13 Pages: 22-9
  • Peer Reviewed
• [Journal Article] Use of postmortem computed tomography to reveal an intraoral gunshot injuries in a charred body (2011)
  • Author(s): Sano R, Hirawasa S, Kobayashi S, Shimada T, Awata S, Takei H, Otake H, Takahashi K, Takahashi Y, Kominato Y
  • Journal Title: Leg Med Volume: 13 Pages: 286-8
  • Peer Reviewed
• [Journal Article] A case of fatal drug intoxication showing a high-density duodenal content by postmortem computed tomography (2011)
  • Author(s): Sano R, Takahashi K, Kominato Y, Araki T, Yamamoto K, Takei H, Otake H, Awata S, Akuzawa H, Tago Y, Aoki H
  • Journal Title: Leg Med Volume: 13 Pages: 39-40
  • Peer Reviewed
• [Journal Article] Use of postmortem computed tomography to reveal an intraoral gunshot injuries in a charred body (2011)
  • Author(s): Sano R, Hirasawa S, Kobayashi S, Shimada T, Awata S, Takei H, Otake H, Takahashi K, Takahashi Y, Kominato Y
  • Journal Title: Leg Med (Tokyo) Volume: 13 Issue: 6 Pages: 286-288
  • DOI: 10.1016/j.legalmed.2011.07.004
  • Peer Reviewed
• [Journal Article] Mutation of the GATA site in the erythroid cell-specific regulatory element of the ABO gene in a Bm subgroup individual
  • Author(s): Nakajima T, Sano R, Takahashi Y, Kubo R, Takahashi K, Kominato Y, Tsukada J, Takeshita H, Yasuda T, Uchikawa M, Isa K, Ogasawara K.
  • Journal Title: Transfusion Volume: (in press)
  • Peer Reviewed
• [Journal Article] Six VKORC1 polymorphims for Asians and its survey on ethnic wide-ranging populations
  • Author(s): Fujihara J, Yasuda T, Kimura-Kataoka K, Sano R, Nakajima T, Kominato Y, Takeshita H
  • Journal Title: DNA Pol Volume: (in press)
  • Peer Reviewed
• [Presentation] ABO式血液型遺伝子エンハンサー欠損がBm型の原因である (2012)
  • Author(s): 佐野利恵,中島たみ子,高橋遥一郎,小湊慶彦
  • Organizer: 日本DNA多型学会第21回学術集会
  • Place of Presentation: 京都
  • Year and Date: 2012-11-08
• [Presentation] ヒトDNaseII遺伝子内プロモーター領域のSNPはプロモーター活性低下によって血清DNaseII活性を減少させる (2012)
  • Author(s): 木村かおり,安田年博,藤原純子,植木美鈴,飯田礼子,佐野利恵,中島たみ子,小湊慶彦,加藤秀章,竹下治男
  • Organizer: 日本DNA多型学会第21回学術集会
  • Place of Presentation: 京都
  • Year and Date: 2012-11-08
• [Presentation] 死後画像検査を加えた、融合型死因調査の一例 (2012)
  • Author(s): 佐野利恵,高橋遥一郎,中島たみ子,小湊慶彦
  • Organizer: 第81次日本法医学会学術関東地方集会
  • Place of Presentation: 群馬
  • Year and Date: 2012-10-20
• [Presentation] ABO式血液型遺伝子のエンハンサーの同定に基づくBm型の遺伝子解析 (2012)
  • Author(s): 高橋遥一郎,佐野利恵,中島たみ子,小湊慶彦,伊藤一人,丸橋隆行,横濱章彦
  • Organizer: 第59回北関東医学会総会
  • Place of Presentation: 群馬
  • Year and Date: 2012-09-27
• [Presentation] ABO式血液型遺伝子のエンハンサーの同定に基づくBm型の遺伝子解析 (2012)
  • Author(s): 中島たみ子,佐野利恵,高橋遥一郎,竹下治男,安田年博,小湊慶彦
  • Organizer: 第96次日本法医学会学術全国集会
  • Place of Presentation: 浜松
  • Year and Date: 2012-06-08
• [Presentation] 死後CT検査により急性硬膜下血腫が見出された,高度の死後変化がある一例 (2012)
  • Author(s): 佐野利恵,高橋遥一郎,中島たみ子,小湊慶彦
  • Organizer: 第96次日本法医学会学術全国集会
  • Place of Presentation: 浜松
  • Year and Date: 2012-06-08
• [Presentation] ABO式血液型遺伝子エンハンサー欠損がBm型の原因である. (2012)
  • Author(s): 佐野利恵, 中島たみ子, 高橋遥一郎, 小湊慶彦.
  • Organizer: 日本ＤＮＡ多型学会第21回学術集会.
  • Place of Presentation: 京都
• [Presentation] ヒトDNase II遺伝子内プロモーター領域のSNPはプロモーター活性低下によって血清 DNase II活性を減少させる. (2012)
  • Author(s): 木村かおり, 安田年博, 藤原純子, 植木美鈴, 飯田礼子, 佐野利恵, 中島たみ子, 小湊慶彦，加藤秀章, 竹下治男.
  • Organizer: 日本ＤＮＡ多型学会第21回学術集会.
  • Place of Presentation: 京都
• [Presentation] ABO式血液型遺伝子のエンハンサーの同定に基づくBm 型の遺伝子解析. (2012)
  • Author(s): 高橋遥一郎, 佐野利恵, 中島たみ子, 小湊慶彦, 伊藤 一人, 丸橋隆行, 横濱章彦.
  • Organizer: 第59回北関東医学会総会.
  • Place of Presentation: 群馬
• [Presentation] ABO式血液型遺伝子のエンハンサーの同定に基づくBm型の遺伝子解析. (2012)
  • Author(s): 中島たみ子, 佐野利恵, 高橋遥一郎, 竹下治男, 安田年博, 小湊慶彦.
  • Organizer: 第96次日本法医学会学術全国集会.
  • Place of Presentation: 浜松
• [Presentation] 死後CT検査により急性硬膜下血腫が見出された,高度の死後変化がある一例. (2012)
  • Author(s): 佐野利恵, 高橋遥一郎, 中島たみ子, 小湊慶彦.
  • Organizer: 第96次日本法医学会学術全国集会.
  • Place of Presentation: 浜松
• [Presentation] ABO式血液型遺伝子3'領域は遺伝子発現の抑制に関与する (2011)
  • Author(s): 佐野利恵,中島たみ子,高橋圭子,窪理英子,矢澤伸,小湊慶彦
  • Organizer: 日本DNA多型学会第20回学術集会
  • Place of Presentation: 神奈川
  • Year and Date: 2011-12-01
• [Presentation] 火災現場から発見された焼損死体で射創が見出された4例における死後CT画像の比較検討 (2011)
  • Author(s): 佐野利恵,中島たみ子,小湊慶彦
  • Organizer: 第80回日本法医学会学術関東地方集会
  • Place of Presentation: 栃木
  • Year and Date: 2011-10-29
• [Presentation] The 3'flanking region of the human ABO gene is involved in negative regulation of gene expression (2011)
  • Author(s): 佐野利恵, 中島たみ子, 高橋圭子, 窪理英子, 矢澤伸, 小湊慶彦
  • Organizer: 第95次日本法医学会学術全国集会
  • Place of Presentation: 福島
  • Year and Date: 2011-06-17
• [Presentation] 死後CT検査において十二指腸内に高吸収域が認められた薬物中毒死亡例 (2011)
  • Author(s): 佐野利恵,高橋圭子,中島たみ子,小湊慶彦
  • Organizer: 第95次日本法医学会学術全国集会
  • Place of Presentation: 福島
  • Year and Date: 2011-06-16
• [Presentation] ABO式血液型遺伝子3’領域は遺伝子発現の抑制に関与する (2011)
  • Author(s): 佐野利恵、中島たみ子、高橋圭子、窪理恵子、矢沢伸、小湊慶彦
  • Organizer: 第20回日本DNA多型学会学術集会
  • Place of Presentation: 横浜
• [Presentation] 火災現場から発見された焼損死体で射創が見出された４例における死後ＣＴ画像の比較検討 (2011)
  • Author(s): 佐野利恵、中島たみ子、小湊慶彦
  • Organizer: 第80回日本法医学会関東地方会
  • Place of Presentation: 宇都宮
• [Presentation] 死後ＣＴ検査において十二指腸内に高吸収域が認められた薬物中毒死亡例 (2011)
  • Author(s): 佐野利恵, 高橋圭子,中島たみ子,小湊慶彦
  • Organizer: 第95次日本法医学会総会
  • Place of Presentation: 福島
• [Presentation] The 3' flankingThe 3' flanking region of the human ABO histo-blood group gene is involved in negative regulation of gene expression. (2011)
  • Author(s): Sano R, Nakajima T, Takahashi K, Kubo R, Yazawa S, Kominato Y.
  • Organizer: 第95次日本法医学会総会
  • Place of Presentation: 福島
• [Presentation] 死後画像検査を加えた、融合型死因調査の一例.
  • Author(s): 佐野利恵, 高橋遥一郎, 中島たみ子, 小湊慶彦.
  • Organizer: 第81次日本法医学会学術関東地方集会.
  • Place of Presentation: 群馬