| Project/Area Number |
23790729
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | SIDS / ニコチン / 変異検出 / 多型解析 / 細胞内応答 |
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| Research Abstract |
The exposure to tobacco smoke is the major risk factor for the occurrence of sudden infant death syndrome (SIDS). A variety of components are present in the smoke, but nicotine is of particular concern in relation to SIDS because of penetrance in the placenta and effects on fetal development. We performed microarray analysis of anadrenal chromaffin (AMC) cell lines treated with nicotine. As the results, 150 geneswere differentially expressed in both the AMC and AMC cell lines treated with nicotine.We are analyzing about these genes. We performed an association study of CYP2A6, CYP1A1, and GSTT1 polymorphisms with SIDS. Moreover, Analysis of hypoxia-related genes and alpha-7 nicotinic receptor gene mutations were performed by direct sequence to the whole coding regions. In these genes, biologically significant mutations were not detected.Our results indicate that the genetic polymorphism of CYP2A6, alpha-7 nicotinic receptor, and hypoxia-related genes are not differ between SIDS victims and control subjects. Moreover, our analysis did not reveal an association between GSTT1 gene deletion or polymorphisms of CYP1A1 and SIDS.
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