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Analysis of the low-dose methamphetamine pre-treatment in the rat midbrain. ; An approach from endoplasmic reticulum stress.

Research Project

Project/Area Number 23790731
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Legal medicine
Research InstitutionKanazawa Medical University

Principal Investigator

TAKEICHI Toshiaki  金沢医科大学, 医学部, 助教 (90460360)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsMethamphetamine / ER stress / Grp78 / ATF6 / ASK1 / Rat / mTOR / AKT / IHC / Western Blotting / apoptosis / 小胞体ストレス / 酸化ストレス / アポトーシス
Research Abstract

We previously reported that low-dose methamphetamine (METH) pre-treatment increases endoplasmic reticulum stress protein levels in the rat midbrain. In this study, we assessed the effects of high-dose METH on rat midbrain. High-dose METH administration led to a significant increase in expression of the endoplasmic reticulum stress protein Grp78 and activated ATF6. Furthermore, we observed an early increase of the antioxidant stress protein SOD1 and increased inhibition of the oxidative stress marker 4-HNE. High-dose METH inhibited the apoptotic index in midbrain. Collectively, these results suggest that low-dose METH pre-treatment influenced the susceptibility of midbrain neurons to high-dose METH administration.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (9 results)

All 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (8 results)

  • [Journal Article] The effects of low-dose methamphetamine pretreatment on endoplasmic reticulum stress and methamphetamine neurotoxicity in the rat midbrain2012

    • Author(s)
      Takeichi T、Wang EL、Kitamura O
    • Journal Title

      Legal Medicin

      Volume: Vol. 14、No.2 Issue: 2 Pages: 69-77

    • DOI

      10.1016/j.legalmed.2011.12.004

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] methamphetamine投与によるラット黒質神経細胞への影響2013

    • Author(s)
      武市敏明
    • Organizer
      第35回日本法医学会学術中部地方集会
    • Place of Presentation
      石川(金沢医科大学病院)
    • Year and Date
      2013-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 少量のメタンフェタミン前投与によりラット中脳ではATF6を介した小胞体ストレス応答が活性化される2013

    • Author(s)
      武市敏明
    • Organizer
      第97次日本法医学会学術全国集会
    • Place of Presentation
      札幌(ロイトン札幌)
    • Year and Date
      2013-06-27
    • Related Report
      2013 Final Research Report
  • [Presentation] The neuroprotective effect of low-dose methamphetamine pretreatment against high-dose methamphetamine neurotoxicity in the rat midbrain is mediated by an unfolded protein response involving ATF62013

    • Author(s)
      Takeichi T
    • Organizer
      Neuro2013
    • Place of Presentation
      京都(国立京都国際会館)
    • Year and Date
      2013-06-20
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 少量のメタンフェタミン前投与によりラット中脳ではATF6を介した小胞体ストレス応答が活性化される2013

    • Author(s)
      武市 敏明、 王 路、 北村 修
    • Organizer
      第97次日本法医学会学術全国集会
    • Place of Presentation
      札幌(ロイトン札幌)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 少量のメタンフェタミン反復投与による小胞体ストレス蛋白の誘導がメタンフェタミン多量投与における神経細胞障害へ与える影響2012

    • Author(s)
      武市敏明
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡(マリンメッセ福岡)
    • Year and Date
      2012-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 少量のメタンフェタミン反復投与による小胞体ストレス蛋白の誘導がメタンフェタミン多量投与における 神経細胞障害へ与える影響2012

    • Author(s)
      武市 敏明
    • Organizer
      弟35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] Methamphetamine投与によるラット黒質神経細胞への影響

    • Author(s)
      武市 敏明、 王 路、 北村 修
    • Organizer
      第35回 日本法医学会学術中部地方集会
    • Place of Presentation
      石川(金沢医科大学病院)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 少量の覚醒剤前投与による小胞体ストレス蛋白質の誘導及び多量覚醒剤投与による神経細胞障害への影響

    • Author(s)
      武市 敏明
    • Organizer
      第95次日本法医学会学術全国集会
    • Place of Presentation
      コラッセふくしま (福島)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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