| Project/Area Number |
23790733
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
UTSUMI Minori 兵庫医科大学, 医学部, 講師 (50351797)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | メタンフェタミン / 覚せい剤 / 線条体 / mRNA / タンパク質 |
|---|
| Research Abstract |
Observing the changes in the density of DA receptor and dopamine transporter expression in rat striatum and nucleus accumbens by chronic methamphetamine (MAP) administration. The real-time PCR and RT-PCR methods, and western-blotting method were used for measurement of m-RNA and protein, respectively. As a result, the DAT-mRNA expression level and the DAT-protein expression level of a chronic MAP administration group were decreased significantly. Decrease in DAT density inhibited the reuptake of DA, which was increased by MAP in the extracellular space. Further, long-term stimulatory effect by DAT inhibition will lead to dependent formation by MAP.
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