The possibility of unknown viral infection with primary biliary cirrhosis and the disease association with anellovirus infection.

• Project/Area Number: 23790765
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Tohoku University
• Principal Investigator: NINOMIYA Masashi 東北大学, 病院, 医員 (70583938)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 肝臓学 / 肝炎ウイルス / microRNA / 次世代シークエンサー / 原発性胆汁性肝硬変

Research Abstract

We tried to sequence hepatitis C virus (HCV) genome with RDV method, before conducting RDV method with serum in PBC patients or NOD. c3c4 mice. But this method seemed to be impossible to detect unknown viral sequence. Therefore, we tried to use deep sequencing technology.
Result: The reads of HCV origin mapped 8583 reads on the reference HCV genome. The reads of HCV origin were detectable in about 0.10% of the total reads. Surprisingly, the coverage showed 98.8% and read depth indicated 69.5x. In NS3, the position of 5 amino acid bases showed mixed variants.

Research Products

• [Journal Article] Murine Models of Nonalcoholic Fatty Liver Disease and Steatohepatitis (2013)
  • Author(s): Ninomiya M, Kondo H, Shimosegawa T
  • Journal Title: ISRN Hepatology Volume: 2013 Pages: 1-7
  • DOI: 10.1155/2013/237870
  • Peer Reviewed
• [Journal Article] Animal Models of Cholangiopathies and Possible Endogenous Viral Infecti (2012)
  • Author(s): Ninomiya M, Ueno Y, Shimosegawa T
  • Journal Title: International journal of hepatology Volume: 2012 Pages: 649290-649290
  • DOI: 10.1155/2012/649290
  • Peer Reviewed
• [Journal Article] Use of illumina deep sequencing technology to differentiate hepatitis Cvirus variants (2012)
  • Author(s): Ninomiya M, Ueno Y, Funayama R, Kagashima T, Nishida Y, Kondo Y, Inoue J, Kakazu E, Kimura O, Nakayama K, Shimosegawa T
  • Journal Title: Journal of clinical microbiology Volume: 50 Issue: 3 Pages: 857-866
  • DOI: 10.1128/jcm.05715-11
  • Peer Reviewed
• [Presentation] 次世代シークエンサーを用いたC型肝炎ウイルスのアミノ酸配列解析と従来のSanger法シークエンスとの比較第48回 (2012)
  • Author(s): 二宮匡史
  • Organizer: 肝臓学会総会
  • Place of Presentation: 金沢
• [Presentation] 次世代シークエンサーを用いて慢性B型肝炎患者血清内の特異的なmiRNAのプロファイリングを行い (2012)
  • Author(s): 二宮匡史
  • Organizer: 新規miRNAの候補を見いだしたJDDW2012
  • Place of Presentation: 神戸
• [Presentation] Characterization of circulating microRNAs in patients with primary biliary cirrhosis by Illumina deep sequencing (2012)
  • Author(s): Ninomiya M
  • Organizer: AASLD
  • Place of Presentation: Boston, USA
• [Presentation] Circulating microRNAs in some patients with viral hepatitis by deep sequencing showed characteristic expression (2012)
  • Author(s): Ninomiya M
  • Organizer: AASLD
  • Place of Presentation: Boston, USA
• [Presentation] 次世代シークエンサーを用いた、C型肝炎ウイルスゲノム解析と血清内 microRNAのプロファイリング (2012)
  • Author(s): 二宮匡史
  • Organizer: 第39回日本肝臓学会東部会
  • Place of Presentation: 東京
• [Presentation] 次世代シークエンサーを用いたC型肝炎ウイルスのアミノ酸配列解析と従来のSanger法シークエンスとの比較 (2012)
  • Author(s): 二宮匡史
  • Organizer: 第48回 肝臓学会総会
  • Place of Presentation: 金沢
• [Presentation] 次世代シークエンサーを用いて慢性B型肝炎患者血清内の特異的なmiRNAのプロファイリングを行い、新規miRNAの候補を見いだした (2012)
  • Author(s): 二宮匡史
  • Organizer: JDDW2012
  • Place of Presentation: 神戸
• [Presentation] Characterization of circulating microRNAs in patients with primary biliary cirrhosis by Illumina deep sequencing. (2012)
  • Author(s): Ninomiya Masashi
  • Organizer: AASLD2012
  • Place of Presentation: Boston, USA
• [Presentation] Circulating microRNAs in some patients with viral hepatitis by deep sequencing showed characteristic expression. (2012)
  • Author(s): Ninomiya Masashi
  • Organizer: AASLD2012
  • Place of Presentation: Boston, USA
• [Presentation] 次世代シークエンサーを用いた、C型肝炎ウイルスゲノム解析と血清内microRNAのプロファイリング (2012)
  • Author(s): 二宮匡史
  • Organizer: 第39回日本肝臓学会東部会
  • Place of Presentation: 東京
• [Presentation] 次世代シークエンサーを用いたC型肝炎ウイルスの解析 (2011)
  • Author(s): 二宮匡史
  • Organizer: JDDW2011
  • Place of Presentation: 福岡
• [Presentation] Efficiency of detecting hepatitis C virus using deep sequencing technology: high faculty to differentiate the viral variants compared with Sanger sequencing (2011)
  • Author(s): Ninomiya M
  • Organizer: AASLD
  • Place of Presentation: San Francisco, USA
• [Presentation] HBc抗体陽性レシピエントに臍帯血移植を施行、HBV再活性化が認められたde novo B型肝炎の1例 (2011)
  • Author(s): 二宮匡史 上野義之 福島耕治 近藤泰輝 井上淳 嘉数英二 涌井祐太 下瀬川徹
  • Organizer: 第193回 日本内科学会東北地方会
  • Place of Presentation: 仙台
• [Presentation] Efficiency of detecting hepatitis C virus using deep sequencing technology: high faculty to differentiate the viral variants compared with Sanger sequencing (2011)
  • Author(s): Masashi Ninomiya, Yoshiyuki Ueno, Ryo Funayama, Yuishiro Nishida, Yasuteru Kondo, Jun Inoue, Eiji Kakazu, Osamu Kimura, Takao Iwasaki, Keiko Nakayama, Tooru Shimosegawa
  • Organizer: AASLD
  • Place of Presentation: アメリカ
• [Book] アークメディア肝胆膵 (2011)
  • Author(s): 二宮匡史、福島耕二、上野義之、下瀬川徹
  • Publisher: ウイルス感染とPBC
• [Book] 肝胆膵 (2011)
  • Author(s): 二宮匡史、福島耕二、上野義之、下瀬川徹
  • Publisher: アークメディア