Fundamental study for intestinal epithelial cell regeneration via regulation of subepithelial myofibroblasts

• Project/Area Number: 23790789
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: BAMBA Shigeki 滋賀医科大学, 医学部, 助教 (40422901)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 腸管上皮再生 / 筋線維芽細胞 / 炎症性腸疾患

Research Abstract

We performed the regulation of subepithelial myofibroblasts to promote regeneration of intestinal epithelial cells. The regulation of subepithelial myofibroblasts is done by introducing antisense oligonucleotide of Foxl1, which is known as mesenchymal transcription factor, via Dectin-1 using schizophyllan (SPG) as an antisense carrier. Inhibition of Foxl1 ameliorate body weight loss and significant increase in length of the colon and crypts in dextran sulfate sodium (DSS) colitis model. Clinical application of antisense Foxl1 coupled with SPG to short bowel syndrome is expected.