The elucidation of the progeny of the Eppk1-expressing cells: insightsinto the pancreatic exocrine regeneration mechanism

• Project/Area Number: 23790797
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Kumamoto University
• Principal Investigator: MIKI Rika 熊本大学, 発生医学研究所, COEリサーチアソシエイト (30516133)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 幹細胞 / 外分泌細胞 / β細胞 / 糖尿病 / 再生医学 / 膵再生 / 膵炎

Research Abstract

Epiplakin (Eppk1) was one of the pancreatic stem cell markers during the development.We hypothesized that Eppk1-expressing cells are one source of regenerating cells.Eppk1-CreER; Z/EG mice were administrated with tamoxifen and then injected caerulein thatcauses acinar cell degeneration. GFP labeled cells were detected in the Eppk1 negativeacinar cells. These result indicated that Eppk1 expressing cells were one source of theregenerated acinar cells.

Research Products

• [Journal Article] Recovery from diabetes in neonatal miceafter a low-dose streptozotocintreatment. (2013)
  • Author(s): Kataoka M., Kawamuro Y., Shiraki N.,Miki R., Sakano D., Yoshida T.,Yasukawa T., Kume K., Kume S.
  • Journal Title: Biochem Biophys ResCommun. Volume: 430(3) Pages: 1103-8
  • URL: http://dx.doi.org/10.1016/j.bbrc.2012.12.030
  • Peer Reviewed
• [Journal Article] Recovery from diabetes in neonatal mice after a low-dose streptozotocin treatment. (2013)
  • Author(s): Kataoka M, Kawamuro Y, Shiraki N, Miki R, Sakano D, Yoshida T, Yasukawa T, Kume K, Kume S.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 430 Issue: 3 Pages: 1103-1108
  • DOI: 10.1016/j.bbrc.2012.12.030
  • Peer Reviewed
• [Presentation] Regeneration of beta cells in neonatalmouse after streptozotocin treatment (2011)
  • Author(s): 片岡 正光、河室 友希、三木 梨可、坂野 大介、白木 伸明、吉田 哲、粂和彦、粂 昭苑
  • Organizer: 第34回分子生物学会
  • Place of Presentation: パシフィコ横浜 横浜
  • Year and Date: 2011-12-13
• [Book] Stem CellBiology: Basic Concepts to FrontiersStudents Edition, Chapter 9.ENDODERM (2012)
  • Author(s): Katsumoto K., Miki R., Umeda, K.,Shiraki, N., Kume, S.
  • Publisher: Dinender K. Singla, PhD, FAHA.Publisher: CreateSpace IndependentPublishing Platform; Student edition