Analysis of vanin-1 in non-alcoholic fatty liver disease

• Project/Area Number: 23790799
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Kagoshima University
• Principal Investigator: YOSHIZAKI Takayuki 鹿児島大学, 産学官連携推進センター, 特任講師 (70515189)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脂肪肝 / 脂肪滴 / 脂質代謝 / 脂質 / 内科 / 栄養学

Research Abstract

We analyzed the expression of hepatic vanin-1 mRNA following the administration of a high-fat diet in mice as well as free fattyacids in hepatocyte cultures and speculated its possible mechanism. Vanin-1 mRNA levels in the livers of mice were upregulated within a day of the high-fat diet. An in vitroanalysis using HuH-7 cells revealed a significant upregulation of vanin-1 mRNA by low oleic acid concentration; however, lipid accumulation in hepatocytes was not affected at this concentration. As a result of examining with various free fatty acids, it was shown that vanin-1 mRNA was upregulated by the oleic acid and the linoleic acid that makes the lipid droplet easily in the hepatocyte.

Research Products

• [Journal Article] Endoplasmic reticulum stress suppresses lipin-1 expression in 3T3-L1 adipocytes (2013)
  • Author(s): Takahashi, N. , Yoshizaki, T., Hiranaka, N., Suzuki, T., Yui, T., Akanuma, M., Kanazawa, K., Yoshida, M., Naito, S., Fujiya, M., Kohgo, Y. and Ieko. M
  • Journal Title: Biochem. Biophys. Res. Comm Volume: 431 Issue: 1 Pages: 25-30
  • DOI: 10.1016/j.bbrc.2012.12.112
  • NAID: 120006522750
  • Peer Reviewed
• [Journal Article] Analysis of vanin-1 upregulation and lipid accumulation in hepatocytes in response to a high-fat diet and free fatty acids (2012)
  • Author(s): Motomura W, Yoshizaki T, Takahashi N, Kumei S, Mizukami Y, Jang SJ, Kohgo Y
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 51 Issue: 3 Pages: 163-169
  • DOI: 10.3164/jcbn.12-06
  • NAID: 130004466691
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes (2011)
  • Author(s): Takahashi, N., Yoshizaki, T., Hiranaka, N., Suzuki, T., Yui, T., Akanuma, M., Oka, K., Kanazawa, K., Yoshida, M., Naito, S., Fujiya, M., Kohgo, Y. and Ieko, M
  • Journal Title: Biochem. Biophys. Res. Comm Volume: 415 Issue: 1 Pages: 200-205
  • DOI: 10.1016/j.bbrc.2011.10.060
  • NAID: 120004461828
  • Peer Reviewed
• [Presentation] 小胞体ストレスは3T3-L1 脂肪細胞のlipin-1 発現を低下させる (2012)
  • Author(s): 高橋伸彦,吉崎隆之,吉田美香,内藤澄悦,高後裕,家子正裕
  • Organizer: 第17回アディポサイエンス研究会シンポジウム
  • Place of Presentation: 千里阪急ホテル(大阪府)
  • Year and Date: 2012-08-25
• [Presentation] 小胞体ストレスは3T3-L1 脂肪細胞におけるlipin-1発現を低下させる (2012)
  • Author(s): 高橋伸彦,吉崎隆之,平中奈津弥,吉田美香,内藤澄悦,高後裕,家子正裕
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2012-05-17
• [Presentation] 高脂肪食投与による脂肪肝形成に伴う肝細胞 Vanin-1 の発現亢進 (2012)
  • Author(s): 吉崎隆之,本村亘,高橋伸彦,粂井志麻,水上裕輔,張成宰,高後裕
  • Organizer: 日本農芸化学会 2012 年度大会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2012-03-23
• [Presentation] 高脂肪食投与による脂肪肝形成に伴う肝細胞Vanin-1の発現亢進 (2012)
  • Author(s): 吉崎隆之
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学（京都府）
• [Presentation] 脂肪細胞における lipin-1 発現低下の意義 (2011)
  • Author(s): 高橋伸彦,吉崎隆之,高後裕,家子正裕
  • Organizer: 第16回アディポサイエンス研究会シンポジウム
  • Place of Presentation: 千里阪急ホテル(大阪府)
  • Year and Date: 2011-08-20
• [Presentation] Suppression of lipin-1 expression by RNAi increases monocyte chemoattractant protein 1 mRNA expression in 3T3-L1 adipocytes (2011)
  • Author(s): 高橋伸彦,吉崎隆之,吉田美香,内藤澄悦,高後裕,家子正裕
  • Organizer: 第43回日本動脈硬化学会総会・学術集会
  • Place of Presentation: ロイトン札幌(北海道)
  • Year and Date: 2011-07-16
• [Presentation] 3T3-L1 脂肪細胞において lipin-1 遺伝子のknock down は MCP-1 遺伝子発現上昇を引き起こす (2011)
  • Author(s): 高橋伸彦,吉崎隆之,高後裕,家子正裕
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌プリンスホテル(北海道)
  • Year and Date: 2011-05-21
• [Presentation] 脂肪細胞におけるlipin-1発現低下の意義 (2011)
  • Author(s): 高橋伸彦
  • Organizer: 第16回アディポサイエンス研究会シンポジウム
  • Place of Presentation: 千里阪急ホテル（大阪府）
• [Presentation] Suppression of lipin-1 expression by RNAi increases monocyte chemoattractant protein 1 mRNA expression in 3T3-L1 adipocytes (2011)
  • Author(s): 高橋伸彦
  • Organizer: 第43回日本動脈硬化学会総会・学術集会
  • Place of Presentation: ロイトン札幌（北海道）
• [Presentation] 3T3-L1脂肪細胞においてlipin-1遺伝子のknock downはMCP-1遺伝子発現上昇を引き起こす (2011)
  • Author(s): 高橋伸彦
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌プリンスホテル（北海道）
• [Presentation] 小胞体ストレスは3T3-L1脂肪細胞のlipin-1発現を低下させる
  • Author(s): 高橋伸彦
  • Organizer: 第17回アディポサイエンス研究会シンポジウム
  • Place of Presentation: 千里阪急ホテル（大阪府）
• [Presentation] 小胞体ストレスは3T3-L1脂肪細胞におけるlipin-1発現を低下させる
  • Author(s): 高橋伸彦
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県）