• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Critical role of hyalurona derived from vascular smooth muscle cells after vascular injury in mice

Research Project

Project/Area Number 23790843
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Circulatory organs internal medicine
Research InstitutionShinshu University

Principal Investigator

KASHIMA Yuichiro  信州大学, 医学部附属病院, 助教(診療) (70545722)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsヒアルロン酸 / 血管平滑筋細胞 / 動脈硬化 / マクロファージ / ステント内再狭窄 / 血管平滑筋
Research Abstract

Inhibition of HA synthesis using 4-methylumbelliferone markedly inhibited neointimal formation after injury. In vitro experiments revealed that low-molecular-weight HA (LMW-HA) induced vascular smooth muscle cells (VSMC) activation, including migration, proliferation, and production of inflammatory cytokines and reactive oxygen species (ROS). The migration and proliferation of VSMCs were mediated by the CD44/RhoA and CD44/ERK1/2 pathways, respectively. Because HA synthase 2 (HAS2) is predominantly expressed in injured arteries, we generated cTg mice that overexpress the murine HAS2 gene specifically in VSMCs (cHAS2/CreSM22 alpha mice) and showed that HA overexpression markedly enhanced neointimal formation after cuff-mediated vascular injury. Further, HA-overexpressing VSMCs isolated from cHAS2/CreSM22alpha mice showed augmented migration, proliferation, and production of inflammatory cytokines and ROS.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (18 results)

All 2013 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (16 results)

  • [Journal Article] Crucial role of hyaluronan in neointimal formation after vascular injury.2013

    • Author(s)
      Kashima Y, Takahashi M, Shiba Y, Itano N, Izawa A, Koyama J, Nakayama J, Taniguchi S, Kimata K, Ikeda U.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 3 Pages: e58760-e58760

    • DOI

      10.1371/journal.pone.0058760

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunoadsorption therapy reduces oxidative stress in patients with dilated cardiomyopathy.2012

    • Author(s)
      Kasai H, Kashima Y, Izawa A, Tomita T, Miyashita Y, Koyama J, Takahashi M, Yoshio T, Yazaki Y, Higuchi M, Ikeda U
    • Journal Title

      WJCD

      Volume: 2 Issue: 04 Pages: 305-312

    • DOI

      10.4236/wjcd.2012.24048

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Critical role of hyaluronan derived from macrophages in neointimal formation after vascular injury in mice2013

    • Author(s)
      Kashima Y, Takahashi M, Kimata K, Itano N, Ikeda U
    • Organizer
      AHA2013アメリカ心臓協会
    • Place of Presentation
      ダラス(アメリカ)
    • Related Report
      2013 Final Research Report
  • [Presentation] Lv remodeling caused by persistent AF was associated with high cardiac hyaluronan in Cs vein2013

    • Author(s)
      Okada A, Tomita T, Kashima Y, Yoshie K, Takeuchi T, Ikeda U
    • Organizer
      AHA2013アメリカ心臓協会
    • Place of Presentation
      ダラス(アメリカ)
    • Related Report
      2013 Final Research Report
  • [Presentation] Crucial role of hyaluronan derived from macrophages in neointimal formation after vascular injury2013

    • Author(s)
      Kashima Y, Takahashi M, Itano N, Taniguchi S, Kimata K, Ikeda U
    • Organizer
      ESC2013欧州心臓学会議
    • Place of Presentation
      アムステルダム(オランダ)
    • Related Report
      2013 Final Research Report
  • [Presentation] Crucial role of hyaluronan derived from macrophages in neointimal formation after vascular injury.2013

    • Author(s)
      Kashima Y, Takahashi M, Itano N, Taniguchi S, Kimata K, Ikeda U.
    • Organizer
      欧州心臓学会議
    • Place of Presentation
      アムステルダム(オランダ)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Critical role of hyaluronan derived from macrophages in neointimal formation after vascular injury in mice.2013

    • Author(s)
      Kashima Y, Takahashi M, Kimata K, Itano N, Ikeda U.
    • Organizer
      米国心臓病学会議
    • Place of Presentation
      ダラス(アメリカ)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Critical role of hyaluronan in neointimal formation after vascular injury in mice2012

    • Author(s)
      Kashima Y, Takahashi M, Shiba Y, Itano N, Kimata K, Izawa A, Ikeda U
    • Organizer
      AHA2012アメリカ心臓協会
    • Place of Presentation
      ロサンゼルス(アメリカ)
    • Related Report
      2013 Final Research Report
  • [Presentation] LV dysfunction caused by persistent atrial fibrillation was associated with high reactive oxidative metabolities in coronary sinus vein2012

    • Author(s)
      Okada A, Tomita T, Kashima Y, Oguchi Y, Takeuchi T, Aizawa K, Ikeda U
    • Organizer
      ESC2012欧州心臓学会議
    • Place of Presentation
      ミュンヘン(ドイツ)
    • Related Report
      2013 Final Research Report
  • [Presentation] 心房細動の持続に伴う左室機能障害は、冠状静脈における酸化ストレスの上昇と関連性を認め、アブレーション治療後血中酸化ストレスは有意に改善する2012

    • Author(s)
      岡田綾子、富田威、嘉嶋勇一郎、小口泰尚、竹内崇博、相澤万象、池田宇一
    • Organizer
      第27回日本不整脈学会学術大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] Critical role of NFκB p65 in pathogenesis of spontaneous in p50 deficient mice2012

    • Author(s)
      Gautam M, Izawa A, Shiba Y, Kashima Y, Koyama J, Hongo M, Sano K, Hayashi T, Ichikawa M, Ikeda U
    • Organizer
      第76回日本循環器学会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] Critical role of hyaluronan in neointimal formation after vascular injury in mice.2012

    • Author(s)
      Kashima Y, Takahashi M, Shiba Y, Itano N, Kimata K, Izawa A, Ikeda U
    • Organizer
      米国心臓病学会議
    • Place of Presentation
      ロサンゼルス
    • Related Report
      2012 Research-status Report
  • [Presentation] Critical Role of Hyaluronan Derived From Vascular Smooth Muscle Cells in Neointimal Formation After Vascular Injury2011

    • Author(s)
      Kashima Y, Takahashi M, Hata T, Kawaguchi M, Izawa A, Itano N, Taniguchi S, Ikeda U
    • Organizer
      AHA2011アメリカ心臓協会
    • Place of Presentation
      オーランド(アメリカ)
    • Related Report
      2013 Final Research Report
  • [Presentation] Specific Overexpression of Hyaluronan in Vascular Smooth Muscle Cells Augments Neointimal Formation after Vascular Injury in Mice2011

    • Author(s)
      Kashima Y, Takahashi M, Hata T, Kawaguchi M, Izawa A, Itano N, Kimata K, Ikeda U
    • Organizer
      第75回日本循環器学会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] The impact of mesurement of reactive oxygen species (ROS) and hydroxyl radicals (-OH) in the pasients of peripheral artery disease2011

    • Author(s)
      Miyashita Y, Kashima Y, Kumazaki S, Aso S, Otagiri K, Saigusa T, Koyama J, Ikeda U
    • Organizer
      第20回日本心血管インターベンション治療学会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] Critical Role of Hyaluronan Derived From Vascular Smooth Muscle Cells in Neointimal Formation After Vascular Injury2011

    • Author(s)
      嘉嶋 勇一郎
    • Organizer
      American Heart Association Scientific Sessionn
    • Place of Presentation
      オーランド(アメリカ)
    • Related Report
      2011 Research-status Report
  • [Presentation] The impact of mesurement of reactive oxygen species (ROS) and hydroxyl radicals (-OH) in the pasients of peripheral artery disease.2011

    • Author(s)
      Miyashita Y, Kashima Y, Kumazaki S, Aso S, Otagiri K, Saigusa T, Koyama J, Ikeda U.
    • Organizer
      第20回日本心血管インターベンション治療学会
    • Place of Presentation
      大阪
    • Related Report
      2011 Research-status Report
  • [Presentation] Specific Overexpression of Hyaluronan in Vascular Smooth Muscle Cells Augments Neointimal Formation after Vascular Injury in Mice.2011

    • Author(s)
      Kashima Y, Takahashi M, Hata T, Kawaguchi M, Izawa A, Itano N, Kimata K, Ikeda U.
    • Organizer
      第75回日本循環器学会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi