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Investigation of the pathogenic mechanism of the cardiomyopathy using the knockout mouse of fatty acid transporter CD36

Research Project

Project/Area Number 23790851
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Circulatory organs internal medicine
Research InstitutionOsaka University

Principal Investigator

MASUDA Daisaku  大阪大学, 医学系研究科, 特任研究員 (20568753)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords心筋 / 脂肪酸代謝 / 糖代謝 / CD36 / FDG-PET / GLUT
Research Abstract

We investigated the relationship between the cardiac function and the energy shift from free fatty acids (FFA) to glucose at a time of cardiac dysfunction using CD36 knockout (CD36KO) mouse, which is a transporter of FFA. The analysis of positron emission tomography revealed that the cardiac uptake of glucose was more increased in CD36KO mice compared with wild-type mouse, along with the overexpression of GLUT -1. By the metabolomics analysis, intracellular levels of acetyl CoA, long chain fatty acid (oleic acid, linoleic acid and palmitic acid), ATP and Phoshpocreatin were higher and the level of citric acid were lower in CD36KO mouse, suggesting that the de novo production of FFA originated from acetyl CoA might be increased. Analyzed by ultrasound cardiography, the heart in CD36KO mouse tended to be hypertropic. After the pressure overload by Thoracic Aortic Constriction (TAC), the weights of heart and lung were increased and the cardiac function got worse from the cardiac hypertrophy to the heart failure. In the heart of the CD36KO mouse, although phosphorylation of S6 ribosomal protein existed, neither apoptosis nor a vascular defect existed. These results suggested that the energy in thecardiomyocyte in CD36KO mouse was in the superfluous state, the energy state failed at an early stage from the state of cardiac hypertrophy to the heart failure when the pressure overload to the heart was performed.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (7 results)

All 2012 2011

All Presentation (7 results)

  • [Presentation] 圧負荷時の CD36欠損マウスにおける心肥大および心機能不全の解析2012

    • Author(s)
      中谷和弘、増田大作、他
    • Organizer
      日本心臓病学会年次学術集会
    • Place of Presentation
      ホテル日航金沢(石川県)
    • Year and Date
      2012-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] hypertrophy and impaired cardiac function of CD36 knockout mice in a pressure overload condition2012

    • Author(s)
      中谷和弘、増田大作、他
    • Organizer
      ESC congress 2012(ヨーロッパ心臓病学会)
    • Place of Presentation
      Internationales Congress Center Munchen (ドイツ、ミュンヘン)
    • Year and Date
      2012-08-29
    • Related Report
      2012 Final Research Report
  • [Presentation] hypertrophy and impaired cardiac function of CD36 knockout mice in a pressure overload condition2012

    • Author(s)
      中谷和弘、増田大作、 他
    • Organizer
      日本動脈硬化学会総会
    • Place of Presentation
      ヒルトン福岡シーホーク(福岡県)
    • Year and Date
      2012-07-19
    • Related Report
      2012 Final Research Report
  • [Presentation] hypertrophy and impaired cardiac function of CD36 knockout mice in a pressure overload condition2012

    • Author(s)
      中谷和弘、増田大作、他
    • Organizer
      日本動脈硬化学会
    • Place of Presentation
      福岡県博多市
    • Related Report
      2012 Annual Research Report
  • [Presentation] hypertrophy and impaired cardiac function of CD36 knockout mice in a pressure overload condition2012

    • Author(s)
      中谷和弘、増田大作、他
    • Organizer
      European Society of Cardiology Congress 2012
    • Place of Presentation
      Munich, Germany
    • Related Report
      2012 Annual Research Report
  • [Presentation] 圧負荷時のCD36欠損マウスにおける心肥大および心機能不全の解析2012

    • Author(s)
      中谷和弘、増田大作、他
    • Organizer
      日本心臓病学会
    • Place of Presentation
      石川県金沢市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Visualization and Quantification of FDG Uptake in Myocardium of CD36-knockout Mice In Vivo Using a High Spatial Resolution Semiconductor PET2011

    • Author(s)
      中谷和弘、増田大作、他
    • Organizer
      第75回日本循環器学会総会・学術集会
    • Place of Presentation
      みなとみらい(横浜)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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