Effect of altered expression of endothelial lipase on plasma lipids and atherosclerosis in rabbits
Project/Area Number |
23790854
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Circulatory organs internal medicine
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Research Institution | Kobe University |
Principal Investigator |
SUN Li 神戸大学, 大学院・医学研究科, 医学研究員 (10547508)
|
Co-Investigator(Renkei-kenkyūsha) |
ISHIDA Tatsuro 神戸大学, 医学部附属病院, 准教授 (00379413)
YASUDA Tomoyuki 神戸大学, 医学部附属病院, 助教 (20457047)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 高比重リポ蛋白 / 炎症 / 脂質代謝 / 血管内皮 / リパーゼ / 動脈硬化 / 家兎 / HDL / 血管内皮リパーゼ / lipase |
Research Abstract |
Endothelial lipase (EL) regulates circulating high-density lipoprotein-cholesterol (HDL-C) levels. There is a discrepancy of EL effect in lipid profiles between mise and humans. Therefore, we investigated the effect of altered EL expression on lipid profile and HDL anti-atherogenic properties in rabbits. Adenovirus encoding human EL (AdhEL) was injected into Japanese white rabbits, which induced hEL overexpression. Consequently, serum HDL-C levels were significantly decreased, while LDL-C levels were not affected. Lipoprotein fractions isolated from humans, rabbits and mice were treated with the EL-rich medium, and measured free fatty acids liberated from the lipoprotein substrates. The hEL hydrolyzed HDL from all three species, while it hydrolyzed mouse LDL much greater than rabbit- and human-LDL. Serum paraoxonase activity, a major component of HDL anti-oxidant properties, significantly reducedin EL-overexpressing rabbits, whereas serum cholesterol efflux capacity did not change despite of the reduced HDL levels. In conclusion, Human EL reduced HDL-C and HDL anti-oxidative property in rabbits. Considering that lipid profile of rabbits resembles that of humans, alteration of EL expression or activity may improve HDLlevels and anti-oxidant properties without affecting LDL-C levels in humans.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Endothelial lipase modulates pressure overload-induced heart failure through alternative pathway for fatty acid uptake2013
Author(s)
Nakajima H, Ishida T, Satomi-Kobayashi S, Mori K, Hara T, Sasaki N, Yasuda T, Toh R, Tanaka H, Kawai H, Hirata K
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Journal Title
Hypertension
Volume: 61(5)
Pages: 1002-7
Related Report
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[Journal Article] Fasting serum concentration of apolipoprotein B48 represents residual risks in patients with new-onset and chronic coronary artery disease.2013
Author(s)
Mori K, Ishida T, Yasuda T, Monguchi T, Sasaki M, Kondo K, Hasokawa M, Nakajima H, Haraguchi Y, Sun L, Shinohara M, Toh R, Nishimura K, Hirata K.
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Journal Title
Clin Chim Acta.
Volume: 421
Pages: 51-56
DOI
Related Report
Peer Reviewed
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[Journal Article] Endothelial lipase mediates HDL levels in normal and hyperlipidemic rabbits2012
Author(s)
Zhang J, Yu Y, Nakamura K, Koike T, Waqar AB, Zhang X, Liu E, Nishijima K, Kitajima S, Shiomi M, Qi Z, Yu J, Graham MJ, Crooke RM, Ishida T, Hirata KI, Hurt-Camejo E, Chen YE, Fan J
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Journal Title
J Atheroscler Thromb
Volume: 19(3)
Pages: 213-26
NAID
Related Report
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