Comprehensive analysis of the changesin role of cardiac progenitor cells during aging

• Project/Area Number: 23790855
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Circulatory organs internal medicine
• Research Institution: Okayama University
• Principal Investigator: YOSHIDA Masashi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70532761)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 心臓内幹細胞 / 老化 / DNA マイクロアレイ / 分子生物学 / DNAマイクロアレイ

Research Abstract

Aims of this study are to clarify the changes in role of cardiac progenitor cells (CPCs) during aging and to improve cardiogenic potential of those CPCs. We found that CPCs from human infant heart already became senescent when compared to those from neonates. This fact indicates CPCs possess potential of progenitor cells, not stem cells. Then we also found that CPCs from neonates had higher cardiogenic activity and CPCs from infants had higher angiogenic activity. DNA microarray analysis revealed that down regulation of insulin-like growth factor-1 receptor lead CPCs to have cardiogenic to angiogenic potential. Our results suggest a functional significance of IGF1R-mediated physiological alteration of human CPC differentiation potential.

Research Products

• [Journal Article] Increased Akt-mTOR signaling in lung epithelium is associated with respiratory distress syndrome in mice (2011)
  • Author(s): Ikeda H, Shiojima I, Oka T, Yoshida M, Maemura K, Walsh K, Igarashi T, Komuro I
  • Journal Title: Mol Cell Biol Volume: 31(5) Issue: 5 Pages: 1054-65
  • DOI: 10.1128/mcb.00732-10
  • Peer Reviewed
• [Journal Article] Prostaglandin I(2) induces apoptosis via upregulation of Fas ligand in pulmonary artery smooth muscle cells from patients with idiopathic pulmonary arterial hypertension (2011)
  • Author(s): Akagi S, Nakamura K, Matsubara H, Fukushima Kusano K, Kataoka N, Oto T, Miyaji K, Miura A, Ogawa A, Yoshida M, Ueda-Ishibashi H, Yutani C, Ito H
  • Journal Title: Int J Cardiol Volume: in press Issue: 3 Pages: 499-505
  • DOI: 10.1016/j.ijcard.2011.09.004
  • Peer Reviewed
• [Journal Article] Intermittent arm ischemia induces vasodilatation of the contralateral upper limb (2011)
  • Author(s): Kenki Enko, Kazufumi Nakamura, Kei Yunoki, Toru Miyoshi, Satoshi Akagi, Masashi Yoshida, Norihia Toh, Mutsuko Sangawa, Nobuhiro Nishii, Satoshi Nagase, Kunihisa Kohno, Hiroshi Morita, Kengo F. Kusano, Hiroshi Ito
  • Journal Title: J Physiol Sci Volume: 61(6) Issue: 6 Pages: 507-13
  • DOI: 10.1007/s12576-011-0172-9
  • NAID: 10030351098
  • Peer Reviewed
• [Presentation] Hedgehog interacting proteicontrols age-dependent angiogenic activity in human cardiac progenitor cells through insulin-like growth factor-1 receptor signaling (2012)
  • Author(s): 吉田賢司
  • Organizer: American Heart Association Scientific Session 2012
  • Place of Presentation: ロサンゼルスn 、カリフォルニア州、アメリカ合衆国
  • Year and Date: 2012-11-03
• [Presentation] Transition in cardiogenic to angiogenic potential of human cardiac progenitor cells occurs with age (2012)
  • Author(s): 吉田賢司
  • Organizer: ISSCR 2012
  • Place of Presentation: 横浜
  • Year and Date: 2012-06-13
• [Presentation] Both insulin-like growth factor-1 and -2 receptor control human cardiac progenitor proliferation adifferentiation in children with congenital heart malformation (2011)
  • Author(s): 吉田賢司
  • Organizer: 2011米国心臓協会学術集nd 会
  • Place of Presentation: 米国、オーランド
  • Year and Date: 2011-11-15
• [Presentation] Both insulin-like growth factor-1 and -2 receptor control human cardiac progenitor proliferation and differentiation in children with congenital heart malformation (2011)
  • Author(s): 吉田賢司
  • Organizer: 2011米国心臓協会学術集会
  • Place of Presentation: 米国、オーランド
• [Presentation] Transition in cardiogenic to angiogenic potential of human cardiac progenitor cells occurs with age
  • Author(s): Masashi Yoshida
  • Organizer: ISSCR 2012
  • Place of Presentation: 横浜
• [Presentation] Hedgehog interacting protein controls age-dependent angiogenic activity in human cardiac progenitor cells through insulin-like growth factor-1 receptor signaling
  • Author(s): Masashi Yoshida
  • Organizer: American Heart Association Scientific Session 2012
  • Place of Presentation: ロサンゼルス, カリフォルニア州, アメリカ合衆国
• [Remarks]
  • URL: http://www.cardio.icn.jp/ht/