The roles of transcriptional regulation of angiogenesis and tissue repair in cell transplantation

• Project/Area Number: 23790870
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Circulatory organs internal medicine
• Research Institution: Nara Medical University
• Principal Investigator: HAYASHI Hisaki 奈良県立医科大学, 医学部, 助教 (30533715)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 血管新生 / 転写調節因子 / 虚血 / シグナル伝達 / 細胞移植 / 下肢虚血 / Notch / Hrt1

Research Abstract

In a mouse model of pathological angiogenesis by hindlimb ischemia (HLI) with femoral artery occlusion, the mRNA expression of Hrt1was markedly induced in ischemic muscle. Hrt1 KO mice do not show overt cardiovascular phenotypes in adulthood, but blood flow recovery in ischemic hindlimb after HLI was significantly blunted compared to WT. Revascularization of ischemic hindlimb was also attenuated in Hrt1 KO mice, as evaluated by capillary density analysis. Accordingly, the severity of ischemic injury such as tissue necrosis and loss is much higher in Hrt1 KO mice than WT. These results suggest that Hrt1 plays important roles in regulating ischemia-induced angiogenesis.

Research Products

• [Presentation] Expression profiles and importance of Hairy-related transcription factor (Hrt) in ischemia-induced angiogenesis (2012)
  • Author(s): 林寿来, 坂部正英, 井岡朋子, 井口朋子, 森岡崇, 佐藤玄基, 稲田賢, 伊藤宗洋, 八反丸美和, Manuel F. Utset,Eric N. Olson, 中川修
  • Organizer: 第35回日本分子生物学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] Hrt転写調節因子の血管発生と血管新生における意義 (2012)
  • Author(s): 森岡崇,林寿来,井岡朋子,坂部正英,木村弘,中川修
  • Organizer: 日本内科学会総会
  • Place of Presentation: 京都
  • Year and Date: 2012-04-14
• [Presentation] 虚血後血管新生におけるHairy-related transcription factor（Hrt）の発現プロファイルと意義の解明 (2012)
  • Author(s): 林寿来
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Hrt転写調節因子の血管発生と血管新生における意義 (2012)
  • Author(s): 森岡崇，林寿来，井岡朋子，坂部正英，木村弘，中川修
  • Organizer: 日本内科学会総会
  • Place of Presentation: みやこめっせ
• [Presentation] Osamu Nakagawa Significance of Hairy-related transcription factor1 in postnatal angiogenesis (2011)
  • Author(s): Hisaki Hayashi, Masahide Sakabe, Tomoko Ioka, Tomoko Fujita, Takashi Morioka, Genki Sato, Ken Inada, Munehiro Ito, Manuel F Utset, Eric N Olson
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-12
• [Presentation] Osamu Nakagawa Significance of Hrt family of transcription factors in postnatal angiogenesis (2011)
  • Author(s): Hisaki Hayashi, Masahide Sakabe, Tomoko Ioka, Takashi Morioka, Genki Sato, Ken Inada
  • Organizer: ISTH2011
  • Place of Presentation: 京都
  • Year and Date: 2011-07-25
• [Presentation] Significance of Hrt family of transcription factors in postnatal angiogenesis (2011)
  • Author(s): Hisaki Hayashi, Masahide Sakabe, Tomoko Ioka, Takashi Morioka, Genki Sato, Ken Inada, Osamu Nakagawa
  • Organizer: XXIII Congress of the International Society on Thrombosis & Haemostasis
  • Place of Presentation: 京都国際会議場
• [Presentation] Significance of Hairy-related transcription factor1 in postnatal angiogenesis (2011)
  • Author(s): Hisaki Hayashi, Masahide Sakabe, Tomoko Ioka, Tomoko Fujita, Takashi Morioka, Genki Sato, Ken Inada, Munehiro Ito, Manuel F Utset, Eric N Olson, Osamu Nakagawa
  • Organizer: 第３４回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜