| Project/Area Number |
23790871
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | National Cardiovascular Center Research Institute (2012-2013)
International University of Health and Welfare (2011) |
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Principal Investigator |
SUGANO Yasuo 独立行政法人国立循環器病研究センター, 病院, 医長 (00317124)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 血管石灰化 / マトリックス蛋白 / 石灰化 / 心血管疾患 / トランスレーショナルリサーチ |
|---|
| Research Abstract |
No difference was observed between transgenic and wild-type mice in mortality, phenotypic characteristics (heart, lung, aorta, kidney, liver) as well as pathologic property at 6 weeks after birth. In addition, expression of mRNA and protein levels of GLA were not significantly increased in produced mice.The conclusion was that transgene expression was inactivated in vivo, although they were expressed in vitro.
The promoter was switched to Tie-2, which also provide vascular-specific transgene expression. However, there were some difficulty in detecting extracellular expression of Gla protein, despite ordinary expression of intracellar extract. Special unexpected processing and binding to other proteins in cells might explain this phenomenon.
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