Genetic analysis of familial atrial fibrillation using next generation sequencer

• Project/Area Number: 23790873
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Circulatory organs internal medicine
• Research Institution: Keio University
• Principal Investigator: AIZAWA Yoshiyasu 慶應義塾大学, 医学部, 助教 (20528145)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 遺伝性不整脈 / イオンチャネル / 心房細動 / 変異 / パッチクランプ法 / 遺伝子解析 / 遺伝子変異 / 不整脈 / 次世代シークエンサー

Research Abstract

We collected blood samples of the patients with familial atrial fibrillation after obtained written informed consent. So far, 11 pedigrees including 7familial and 4 younger cases were included. The DNAs of these patients were sequenced for KCNQ1, KCNH2, S

Research Products

• [Journal Article] Narrow QRS tachycardia : Mechanism behind changes in conduction (2013)
  • Author(s): Aizawa Y, Takatsuki S, Inagawa K, Katsumata Y, Nishiyama T, Kimura T, Nishiyama N, Sato Y, Fukumoto K, Tanimoto Y, Tanimoto K, Fukuda K
  • Journal Title: Herz Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Ventricular fibrillation associated with complete right bundle branch block. (2013)
  • Author(s): Aizawa Y, Takatsuki S, Kimura T, Nishiyama N, Fukumoto K, Tanimoto Y, Tanimoto K, Miyoshi S, Suzuki M, Yokoyama Y, Chinushi M, Watanabe I, Ogawa S, Aizawa Y, Antzelevitch C, Fukuda K
  • Journal Title: Heart Rhythm. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Ridge-Related Reentry: A Variant of Perimitral Atrial Tachycardia (2013)
  • Author(s): Takatsuki S, Fukumoto K, Igawa O, Kimura T, Nishiyama N, Aizawa Y, Tanimoto Y, Tanimoto K, Miyoshi S, Fukuda K
  • Journal Title: J Cardiovasc Electrophysiol Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Anatomical characteristics of the left atrial appendage in cardiogenic stroke with low CHADS2 scores (2013)
  • Author(s): Kimura T, Takatsuki S, Inagawa K, Katsumata Y, Nishiyama T, Nishiyama N, Fukumoto K, Aizawa Y, Tanimoto Y, Tanimoto K, Jinzaki M, Fukuda K
  • Journal Title: Heart Rhythm Volume: 10(6) Pages: 921-925
  • Peer Reviewed
• [Journal Article] Successful radiofrequency catheter ablation of atrioventricular nodal reentrant tachycardia in a patient with dextrocardia and situs inversus (2013)
  • Author(s): Aizawa Y, Takatsuki S, Kimura T, Nishiyama N, Fukumoto K, Tanimoto Y, Tanimoto K, Fukuda Y, Miyoshi S, Fukuda K
  • Journal Title: Herz. Volume: 38(1) Pages: 102-4
  • Peer Reviewed
• [Journal Article] Three-dimensional imaging and mapping of the right and left phrenic nerves: relevance to interventional cardiovascular therapy. (2013)
  • Author(s): Fukumoto K, Takatsuki S, Jinzaki M, Yamada M, Tanimoto K, Nishiyama N, Aizawa Y, Hagiwara Y, Fukuda Y, Kimura T, Miyoshi S, Kuribayashi S, Fukuda K
  • Journal Title: Europace. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Ventricular fibrillation associated with complete right bundle branch block. (2013)
  • Author(s): Yoshiyasu Aizawa
  • Journal Title: Heart Rhythm Volume: in press Issue: 7 Pages: 1028-1035
  • DOI: 10.1016/j.hrthm.2013.03.013
• [Journal Article] Narrow QRS tachycardia : Mechanism behind changes in conduction. (2013)
  • Author(s): Yoshiyasu Aizawa
  • Journal Title: Herz Volume: in press
  • Peer Reviewed
• [Journal Article] Successful radiofrequency catheter ablation of atrioventricular nodal reentrant tachycardia in a patient with dextrocardia and situs inversus. (2013)
  • Author(s): Yoshiyasu Aizawa
  • Journal Title: Herz Volume: 38 Issue: 1 Pages: 102-104
  • DOI: 10.1007/s00059-012-3666-1
  • Peer Reviewed
• [Journal Article] Extreme QT prolongation during therapeutic hypothermia after cardiac arrest due to long QT syndrome (2012)
  • Author(s): Nishiyama N, Sato T, Aizawa Y, Nakagawa S, Kanki H.
  • Journal Title: Am J Emerg Med Volume: 30(4)
• [Journal Article] Prevalence and distribution of sarcomeric gene mutations in Japanese patients with familial hypertrophic cardiomyopathy (2012)
  • Author(s): Otsuka H, Arimura T, Abe T, Kawai H, Aizawa Y, Kubo T, Kitaoka H, Nakamura H, Nakamura K, Okamoto H, Ichida F, Ayusawa M, Nunoda S, Isobe M, Matsuzaki M, Doi YL, Fukuda K, Sasaoka T, Izumi T, Ashizawa N, Kimura A
  • Journal Title: Circ J. Volume: 76(2) Pages: 453-61
  • NAID: 10030035969
  • Peer Reviewed
• [Journal Article] Trans-telephonic ICD alert due to recommended replacement time notification: What is the problem? (2012)
  • Author(s): Aizawa Y, Negishi M, Fukuda Y, Takatsuki S
  • Journal Title: Int J Cardiol Volume: 159(1)
  • Peer Reviewed
• [Journal Article] Characteristics of electrocardiographic repolarization in acute myocardial infarction complicated by ventricular fibrillation. (2012)
  • Author(s): Aizawa Y, Jastrzebski M, Ozawa T, Kawecka-Jaszcz K, Kukla P, Mitsuma W, Chinushi M, Ida T, Aizawa Y, Ojima K, Tagawa M, Fujita S, Okabe M, Tsuchida K, Miyakita Y, Shimizu H, Ito S, Imaizumi T, Toba K.
  • Journal Title: J Electrocardiol Volume: 45(3) Pages: 252-9
  • Peer Reviewed
• [Journal Article] Dynamicity of the J-wave in idiopathic ventricular fibrillation with a special reference to pause-dependent augmentation of the J-wave (2012)
  • Author(s): Aizawa Y, Sato A, Watanabe H, Chinushi M, Furushima H, Horie M, Kaneko Y, Imaizumi T, Okubo K, Watanabe I, Shinozaki T, Aizawa Y, Fukuda K, Joo K, Haissaguerre M
  • Journal Title: J Am Coll Cardiol Volume: 59(22) Pages: 1948-53
  • Peer Reviewed
• [Journal Article] Disease characterization using LQTS-specificinduced pluripotent stem cells (2012)
  • Author(s): Egashira T, Yuasa S, Suzuki T, Aizawa Y, Yamakawa H, Matsuhashi T, Ohno Y, Tohyama S, Okata S, Seki T, Kuroda Y, Yae K, Hashimoto H, Tanaka T, Hattori F, Sato T, Miyoshi S, Takatsuki S, Murata M, Kurokawa J, Furukawa T, Makita N, Aiba T, Shimizu W, Horie
  • Journal Title: Cardiovasc Res. Volume: 95(4) Pages: 419-29
  • Peer Reviewed
• [Journal Article] Electrical isolation of the superior vena cava using upstream phrenic pacing to avoid phrenic nerve injury (2012)
  • Author(s): Kimura T, Takatsuki S, Fukumoto K, Nishiyama N, Sato Y, Aizawa Y, Fukuda Y, Sato T, Miyoshi S, Fukuda K
  • Journal Title: Pacing Clin Electrophysiol Volume: 35(9) Pages: 1053-60
  • Peer Reviewed
• [Journal Article] Implantation of the right ventricular lead of an implantable cardioverter-defibrillator complicated by apical myocardial infarction. (2012)
  • Author(s): Nishiyama N, Takatsuki S, Kimura T, Aizawa Y, Fukumoto K, Hagiwara Y, Tanimoto K, Fukuda K
  • Journal Title: Circulation Volume: 126(10) Pages: 1314-5
  • Peer Reviewed
• [Journal Article] Trans-telephonic ICD alert due to recommended replacement time notification: What is the problem? (2012)
  • Author(s): Yoshiyasu Aizawa
  • Journal Title: Int J Cardiol Volume: 159 Issue: 1 Pages: e18-e19
  • DOI: 10.1016/j.ijcard.2011.11.008
• [Journal Article] Disease characterization using LQTS-specific induced pluripotent stem cells. (2012)
  • Author(s): Egashira T, Yuasa S, Suzuki T, Tohyama S, et al.
  • Journal Title: Cardiovasc. Res. Volume: 95 Issue: 4 Pages: 419-29
  • DOI: 10.1093/cvr/cvs206
  • Peer Reviewed
• [Journal Article] Dynamicity of the J wave in idiopathic ventricular fibrillation with a special reference to pause-dependent augmentation of the J wave. (2012)
  • Author(s): Aizawa Y
  • Journal Title: Journal of American College of Cardiology Volume: 59 Issue: 22 Pages: 1948-53
  • DOI: 10.1016/j.jacc.2012.02.028
  • Peer Reviewed
• [Journal Article] Notch on the T wave (2011)
  • Author(s): Aizawa Y Tanaka T, Fukuda K, Funazaki T.
  • Journal Title: Intern Med. Volume: 50(12) Pages: 1353-1353
  • NAID: 130000770507
  • Peer Reviewed
• [Journal Article] Trans-telephonic ICD alert due to recommended replacement time notification: What is the problem? (2011)
  • Author(s): Aizawa Y, Negishi M, Fukuda Y, Takatsuki S
  • Journal Title: Int J Cardiol. Volume: [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Prevalence and distribution of sarcomeric gene mutations in Japanese patients with familial hypertrophic cardiomyopathy. (2011)
  • Author(s): Otsuka H, Arimura T, Abe T, Kawai H, Aizawa Y, et al.
  • Journal Title: Circ J. Volume: 76(2) Pages: 453-61
  • NAID: 10030035969
  • Peer Reviewed
• [Journal Article] Notch on the T wave. (2011)
  • Author(s): Aizawa Y, Tanaka T, Fukuda K, Funazaki T.
  • Journal Title: Intern Med. Volume: 50(12) Pages: 1353-1353
  • NAID: 130000770507
  • Peer Reviewed
• [Journal Article] Extreme QT prolongation during therapeutic hypothermia after cardiac arrest due to long QT syndrome. (2011)
  • Author(s): Nishiyama N, Sato T, Aizawa Y, Nakagawa S, Kanki H.
  • Journal Title: Am J Emerg Med. Volume: [Epub ahead of print]
  • Peer Reviewed
• [Journal Article] A danger of induction of Brugada syndrome during pill-in-the-pocket therapy for paroxysmal atrial fibrillation (2010)
  • Author(s): Aizawa Y, Matsuhashi T, Sato T, Takatsuki S, Fukuda K
  • Journal Title: Drug Healthc Patient Saf Volume: 2 Pages: 139-40
  • Peer Reviewed
• [Presentation] Clinical and Electrocardiographic Characteristics of Right Bundle Branch Block in Patients with Brugada Syndrome (2013)
  • Author(s): Yoshiyasu Aizawa, Seiji Takatsuki , Kohei Inagawa, Yoshinori Katsumata, Takahiko Nishiyama, Takehiro Kimura, Nobuhiro Nishiyama, Kotaro Fukumoto, Yoko Tanimoto, Kojiro Tanimoto, Shun Kohsaka, Mitsushige Murata, Shunichiro Miyoshi, Hideo Mitamura, Satoshi
  • Organizer: 第77回日本循環器学会学術集会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] デバイスで検出された心房細動の患者背景と臨床的特徴の検討 (2013)
  • Author(s): 相澤義泰、高月誠司、根岸壮親、大石愛光、冨永浩史、稲川浩平、勝俣良紀、西山崇比古、木村雄弘、西山信大、福本耕太郎、谷本陽子、谷本耕司郎、福田恵一
  • Organizer: 第5回植込みデバイス関連冬季大会
  • Place of Presentation: 東京都千代田区
• [Presentation] Brugada症候群および特発性心室細動患者に対するベプリジルの不整脈イベント抑制効果に関する検討 (2012)
  • Author(s): 相澤義泰、樫村晋、稲川浩平、勝俣良紀、西山崇比古、木村雄弘 、西山信大、福本耕太郎、谷本陽子、谷本耕司郎、高月誠司、福田恵一
  • Organizer: 第7回Arrhythmia Frontier (AF)
  • Place of Presentation: 東京
  • Year and Date: 2012-12-01
• [Presentation] 家族性WPW症候群の1家系における臨床像の検討 (2012)
  • Author(s): 稲川浩平 、相澤義泰 、高月誠司、勝俣良紀 、西山崇比古 、木村雄弘 、西山信大 、福本耕太郎 、谷本陽子 、谷本耕司郎 、湯浅慎介 、西森健雄 、稲垣雅行 、有村卓朗、木村彰方 、三田村秀雄 、福田恵一
  • Organizer: 第29回日本心電学会学術集会
  • Year and Date: 2012-10-12
• [Presentation] 動悸精査における携帯型心電計(カルジオホン)の不整脈診断能力に関する検討 (2012)
  • Author(s): 相澤義泰、高月誠司 、木村雄弘 、西山信大 、福本耕太郎 、谷本陽子 、谷本耕司郎 、香坂俊 、福田有希子 、村田光繁 、三好俊一郎 、佐藤俊明 、岩永史郎 、小 川 聡 、福田恵一
  • Organizer: 第60回日本心臓病学会学術集会
  • Year and Date: 2012-09-16
• [Presentation] 完全右脚ブロックを呈する特発性心室細動症例の臨床的および心電図学的特徴の検討 (2012)
  • Author(s): 相澤義泰, 高月誠司, 木村雄弘, 西山信大, 福本耕太郎, 谷本陽子, 谷本耕司郎, 三好俊一郎, 鈴木誠, 横山泰廣, 池主雅臣, 相澤義房, 福田恵一
  • Organizer: 第27回日本不整脈学会学術集会
  • Place of Presentation: 神奈川県横浜
  • Year and Date: 2012-07-06
• [Presentation] Diagnostic Value of Portable Electrocardiogram (Cardiophone) in Patients Complaining of Palpitations (2012)
  • Author(s): Yoshiyasu Aizawa, Seiji Takatsuki, Kohei Inagawa, Yoshinori Katsumata, Takahiko Nishiyama, Takehiro Kimura, Nobuhiro Nishiyama, Kotaro Fukumoto, Yoko Tanimoto, Kojiro Tanimoto, Toshiaki Sato, Satoshi Ogawa, Keiichi Fukuda
  • Organizer: APHRS
• [Presentation] Diagnostic Value of Portable Electrocardiogram (Cardiophone) in Patients Complaining of Palpitations (2012)
  • Author(s): Yoshiyasu Aizawa
  • Organizer: 5th Asia Pacific Heart Rhythm Society Scientific Session
  • Place of Presentation: Taipei, Taiwan
• [Presentation] 動悸精査における携帯型心電計（ カルジオホン）の不整脈診断能力に関する検討 (2012)
  • Author(s): 相澤義泰
  • Organizer: 第60回日本心臓病学会学術集会
  • Place of Presentation: 石川県金沢市
• [Presentation] 完全右脚ブロックを呈する特発性心室細動症例の臨床的および心電図学的特徴の検討 (2012)
  • Author(s): 相澤義泰
  • Organizer: 第27回日本不整脈学会学術集会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] 非心不全患者におけるリモートモニタリングによる胸郭インピーダンス測定の意義に関する検討． (2012)
  • Author(s): 相澤義泰
  • Organizer: 第4回植込みデバイス関連冬季大会．
  • Place of Presentation: 北九州国際会議場(福岡県)．
• [Presentation] 診断に苦慮し、DDDペースメーカ植込みにより症状の著明な改善を得た再発性失神の1例． (2012)
  • Author(s): 田部井亮太、相澤義泰他．
  • Organizer: 第4回植込みデバイス関連冬季大会．
  • Place of Presentation: 北九州国際会議場(福岡県)．
• [Presentation] 遠隔モニタリングの詳細なチェックにより患者の死亡を早期に発見出来た1例． (2012)
  • Author(s): 根岸壮親、相澤義泰他．
  • Organizer: 第4回植込みデバイス関連冬季大会．
  • Place of Presentation: 北九州国際会議場(福岡県)．
• [Presentation] 特発性心室細動のVFストームに対し心内膜側からのカテーテルアブレーションを試みた1例． (2012)
  • Author(s): 相澤義泰
  • Organizer: 第10回 特発性心室細動研究会(J-IVFS)．
  • Place of Presentation: 大手町サンケイプラザ(東京都)．
• [Presentation] Significance of Daily Thoracic Impedance Measurement in Patients without Heart Failure: is it Really Necessary? (2012)
  • Author(s): 相澤義泰
  • Organizer: 第76回日本循環器学会学術集会．
  • Place of Presentation: 福岡国際会議場(福岡県)．
• [Presentation] デバイス遠隔モニタリング時のアラート発生とその詳細に関する検討． (2012)
  • Author(s): 冨永浩史、相澤義泰他
  • Organizer: 第76回日本循環器学会学術集会．
  • Place of Presentation: 福岡国際会議場(福岡県)．
• [Presentation] 『院外心肺停止にて発症、VF除細動直後に著明なJ波を認めたJ wave syndromeの1例』 (2011)
  • Author(s): 相澤義泰
  • Organizer: 第4回Arrhythmia Frontier (AF).
  • Place of Presentation: 御茶ノ水ファーストビル(東京都)．
• [Presentation] 2本の副伝導路があり、1つは過常伝導特性を呈したWPW症候群の一例． (2011)
  • Author(s): 相澤義泰
  • Organizer: 第1回関東アブレーションフロンティア．
  • Place of Presentation: アキバプラザ(東京都)．
• [Presentation] Long-term Predictors of ICD Shocks in Patients with Brugada Syndrome. (2011)
  • Author(s): Aizawa Y
  • Organizer: 第75回日本循環器学会学術集会．
  • Place of Presentation: パシフィコ横浜(神奈川県)．
• [Presentation] A Case of J Wave Syndrome Successfully Resuscitated from Cardiac Arrest Followed by Prominent J Wave Augmentation. (2011)
  • Author(s): Aizawa Y
  • Organizer: 4th Asia Pacific Heart Rhythm Society Scientific Session (APHRS).
  • Place of Presentation: ヒルトン福岡シーホーク(福岡県)．
• [Presentation] Successful Radiofrequency Catheter Ablation of Atrioventricular Nodal Reentrant Tachycardia in a Patient with Dextrocardia and Situs Invertus. (2011)
  • Author(s): Aizawa Y
  • Organizer: 4th Asia Pacific Heart Rhythm Society Scientific Session (APHRS).
  • Place of Presentation: ヒルトン福岡シーホーク(福岡県)．
• [Presentation] ．『ICDストームを再発し、イソプロテレノール点滴静注がVF抑制に有効であった特発性心室細動の1例』 (2011)
  • Author(s): 相澤義泰
  • Organizer: 第10回平岡不整脈研究会．
  • Place of Presentation: KKRホテル熱海(静岡県)．