Development of new treatment for fatal ventricular tachyarrhythmias using heavy ion irradiation

• Project/Area Number: 23790880
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Circulatory organs internal medicine
• Research Institution: Tokai University
• Principal Investigator: AMINO Mari 東海大学, 医学部, 講師 (10407976)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: コネキシン43 / 重粒子線照射 / 心室遅延電位 / 心室遅延電 / コネキシン / 心機能 / 心筋梗塞モデル / 致死性心室性不整脈

Research Abstract

The aim of this study is to investigate longitudinal changes in electromechanical performance of myocardial infarction (MI) dog hearts by targeted heavy ion irradiation (THIR). Four dogs were treated with THIR (12C6r, 15Gy; RIKEN,Japan) 2W after MI. Signal-averaged ECGs and echocardiograms were obtained longitudinally over 1 yr. Inducibility of fatal ventricular tachyarrhythmias (VT/VF) and connexin 43(Cx43) immunolabeling of the left ventricular tissues were studied 1 yr after MI. Immunolabeled Cx43 were significantly increased in dogs with THIR at non-ischemic zone and ischemic-border zone. Filtered-QRS increased 2W after MI and remained unchanged up to 1 yr in dogs without THIR, while returned toward baseline in dogs with THIR. RMS40 reduced 2W after MI, and then returned toward baseline only in dogs with THIR. VT/VFs were induced in 1/4 with THIR, whereas in 4/4 without THIR. LV systolic function inhibited 2W after MI, however 1 yr recovery was significantly enhanced by THIR.

Research Products

• [Presentation] Heavy ion irradiation after myocardial infarction facilitates long-term recovery of electromechanical synchrony of canine hearts in association with Cx43 upregulation (2013)
  • Author(s): Mari Amino
  • Organizer: 日本循環器学会学術集会
• [Presentation] 重粒子線照射が梗塞後の不整脈基質に及ぼす長期効果 : イヌ心筋梗塞モデルを用いた検討 (2012)
  • Author(s): 網野真理
  • Organizer: 日本心電学会学術集会
  • Place of Presentation: ホテルニューオータニ幕張
• [Presentation] 重粒子線照射が梗塞後の不整脈基質に及ぼす長期効果：イヌ心筋梗塞モデルを用いた検討 (2012)
  • Author(s): 網野真理
  • Organizer: 日本心電学会学術集会
  • Place of Presentation: ホテルニューオータニ幕張
• [Book] 不整脈学 (2012)
  • Author(s): 網野真理(分担著者)
  • Publisher: 南江堂
• [Book] 不整脈学 (2012)
  • Author(s): 網野真理 分担著者
  • Total Pages: 614
  • Publisher: 南江堂