Regulation of Endothelial Function by ROCK2
| Project/Area Number |
23790882
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
KAWANAMI DAIJI 東京慈恵会医科大学, 医学部, 講師 (50568889)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 動脈硬化 / 血管内皮 / Ｒｈｏ－ｋｉｎａｓｅ / Rho / ROCK2 / ROCK / 小胞体ストレス |
|---|
| Research Abstract |
The process of atherosclerosis is affected by interactions among numerous biological pathways. Accumulating evidence shows that small GTP-binding protein Rho and its effector Rho-kinase (ROCK) play an important role in the development of atherosclerosis. ROCK has two isoforms, ROCK1 and ROCK2. However, it is unclear how ROCK2 regulates endothelial function. To this end, we first examined the effect of ROCK inhibitors on endothelial function. We found that ROCK inhibitors such as Y-27632 and fasudil attenuates endothelial inflammation markers including MCP-1. Furthermore, knockdown of ROCK2 reduced MCP-1 expression levels in endothelial cells. Finally, we isolated peritoneal macrophages from ROCK2-deficient and wild-type mice. LPS induced MCP-1 expression in the cells derived from wild-type. Interestingly, induction of MCP-1 was inhibited in ROCK2-deficient cells. These finding indicate that ROCK2 plays an important role in the development of atherosclerosis.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Rho-kinase inhibition prevents the progression of diabetic nephropathy by downregulating hypoxia-inducible factor 1α2013
Author(s)
Matoba K, Kawanami D, Okada R, Tsukamoto M, Kinoshita J, Ito T, Ishizawa S, Kanazawa Y, Yokota T, Murai N, Matsufuji S, Takahashi-Fujigasaki J, Utsunomiya K
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Journal Title
Kidney Int
Volume: 84
Pages: 545-54
Related Report
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[Journal Article] Rho-kinase inhibition prevents the progression of diabetic nephropathy by downregulating hypoxia-inducible factor 1a2013
Author(s)
Matoba K, Kawanami D, Okada R, Tsukamoto M, Kinoshita J, Ito T, Ishizawa S, Kanazawa Y, Yokota T, Murai N, Matsufuji S, Takahashi-Fujigasaki J, Utsunomiya K
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Journal Title
Kidney International
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Kruppel-like factor 15 regulates skeletal muscle lipid flux and exercise adaptation2012
Author(s)
Haldar SM, Jeyaraj D, Anand P, Zhu H, Lu Y, Prosdocimo DA, Eapen B, Kawanami D, Okutsu M, Brotto L, Fujioka H, Kerner J, Rosca MG, McGuinness OP, Snow RJ, Russell AP, Gerber AN, Bai X, Yan Z, Nosek TM, Brotto M, Hoppel CL, Jain MK
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Journal Title
Proc Natl Acad Sci U S A
Volume: 109
Pages: 6739-44
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