Research Project
Grant-in-Aid for Young Scientists (B)
The process of atherosclerosis is affected by interactions among numerous biological pathways. Accumulating evidence shows that small GTP-binding protein Rho and its effector Rho-kinase (ROCK) play an important role in the development of atherosclerosis. ROCK has two isoforms, ROCK1 and ROCK2. However, it is unclear how ROCK2 regulates endothelial function. To this end, we first examined the effect of ROCK inhibitors on endothelial function. We found that ROCK inhibitors such as Y-27632 and fasudil attenuates endothelial inflammation markers including MCP-1. Furthermore, knockdown of ROCK2 reduced MCP-1 expression levels in endothelial cells. Finally, we isolated peritoneal macrophages from ROCK2-deficient and wild-type mice. LPS induced MCP-1 expression in the cells derived from wild-type. Interestingly, induction of MCP-1 was inhibited in ROCK2-deficient cells. These finding indicate that ROCK2 plays an important role in the development of atherosclerosis.
All 2013 2012 2011 Other
All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (9 results) Remarks (1 results)
Biochem Biophys Res Commun
Volume: 435 Pages: 171-5
Exp Neurol
Volume: 247 Pages: 438-46
Kidney Int
Volume: 84 Pages: 545-54
Biochemical and Biophysical Research Communications
Volume: 435 Issue: 2 Pages: 171-175
10.1016/j.bbrc.2013.04.091
Kidney International
Volume: 印刷中
Proc Natl Acad Sci U S A
Volume: 109 Pages: 6739-44
Volume: 411 Pages: 798-803
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