| Project/Area Number |
23790889
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 心筋症 / リン酸化プロテオミクス / 定量プロテオミクス / プロテオミクス / シグナルネットワーク / LC-MS/MS / 心不全 |
|---|
| Research Abstract |
In this study, a novel method for reproducible, unbiased, and quantitative extraction of tissue proteins was successfully developed. Using this method combined with an optimized protocol for phosphopeptide enrichment and LC-MS/MS analysis, more than 6,000 phosphopeptides were identified and quantified from two kinds of mouse models for cardiomyopathy and a control one, and 20% of the identified peptides showed more than two-fold changes in MS signals. From these data, I am attempting to identify specific signal networks closely related to the onset and development of cardiomyopathy.
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