| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
To identify the pirfenidone targeting molecule, I conducted the affinity purification from culture cell lysates. RAW246.7, PC-9 and A549 culture cells were cultured to obtain the cytosolic and nuclear protein fractions. The affinity purification from RAW246.7lysate and subsequent SDS-PAGE and silver stain showed the candidates of the pirfenidone binding protein. But the repetitive experiment did not present the identical candidate proteins. The affinity purification from PC-9 lysate revealed the candidate of the pirfenidone binding protein. The pirfenidone targeting molecule could not be identified by the mass spectrometry due to the insufficiency of the amount of the candidate protein.
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