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Identification of the pirfenidone targeting molecule

Research Project

Project/Area Number 23790893
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Respiratory organ internal medicine
Research InstitutionTohoku University

Principal Investigator

SAKAKIBARA Tomohiro  東北大学, 病院, 助教 (80422111)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords非閉塞性肺疾患 / 肺線維症 / ピルフェニドン
Research Abstract

To identify the pirfenidone targeting molecule, I conducted the affinity purification from culture cell lysates. RAW246.7, PC-9 and A549 culture cells were cultured to obtain the cytosolic and nuclear protein fractions. The affinity purification from RAW246.7lysate and subsequent SDS-PAGE and silver stain showed the candidates of the pirfenidone binding protein. But the repetitive experiment did not present the identical candidate proteins. The affinity purification from PC-9 lysate revealed the candidate of the pirfenidone binding protein. The pirfenidone targeting molecule could not be identified by the mass spectrometry due to the insufficiency of the amount of the candidate protein.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (12 results)

All 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (6 results)

  • [Journal Article] Randomized phase II trial of uracil/tegafur and cisplatin versusvinorelbine and cisplatin with concurrent thoracic radiotherapy for locally advanced unresectable stage III non-small-cell lung cancer:NJLCG 06012013

    • Author(s)
      Sugawara S, Maemondo M, Tachihara M, Inoue A, Ishimoto O, Sakakibara T, 全14名中6番目
    • Journal Title

      Lung Cancer

      Volume: 81 Issue: 1 Pages: 1-1

    • DOI

      10.1016/j.lungcan.2013.04.010

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A phase II study of irinotecan as a third- or fourth-line treatment for advanced non-small cell lung cancer: NJLCG07032013

    • Author(s)
      Matsubara M
    • Journal Title

      Respir Investig.

      Volume: 51 Issue: 1 Pages: 28-34

    • DOI

      10.1016/j.resinv.2012.09.004

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bilateral peripheral infiltrates refractory to immunosuppressants were diagnosed as autoimmune pulmonary alveolar proteinosis and improved by inhalation of granulocyte/macrophage-colony stimulating factor2012

    • Author(s)
      Satoh H, Tazawa R, Sakakibara T,
    • Journal Title

      Intern Med

      Volume: 51 Pages: 1737-42

    • NAID

      130002062188

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Complicated secondary pneumonia after swine-origin influenza A virus infection in an immunocompetent patient2012

    • Author(s)
      Igusa R, Sakakibara T,
    • Journal Title

      Tohoku J Exp Med

      Volume: 226 Pages: 117-20

    • NAID

      10030197117

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bilateral peripheral infiltrates refractory to immunosuppressants were diagnosed as autoimmune pulmonary alveolar proteinosis and improved by inhalation of granulocyte/macrophage-colony stimulating factor.2012

    • Author(s)
      Satoh H
    • Journal Title

      Intern Med.

      Volume: 51 Pages: 1737-1742

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新型インフルエンザに罹患した健常者成人発症した二次性肺炎2012

    • Author(s)
      井草龍太郎、榊原智博、柴原泰三ほか
    • Journal Title

      Tohoku J Exp Med.

      Volume: 226 Pages: 117-120

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Whole-exome sequencing of familial non-small cell lung cancer patients with the EGFR genemutaions2012

    • Author(s)
      東出直樹、菊地利明、榊原智博、一ノ瀬正和
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      2012-09-21
    • Related Report
      2012 Final Research Report
  • [Presentation] EGFR遺伝子変異を伴う肺腺癌患者における遺伝学的機序の解析2012

    • Author(s)
      東出直樹、菊地利明、榊原智博、一ノ瀬正和
    • Organizer
      第52回日本呼吸器学会学術講演会
    • Place of Presentation
      神戸
    • Year and Date
      2012-04-22
    • Related Report
      2012 Final Research Report
  • [Presentation] EGFR遺伝子変異を伴う肺腺癌患者における遺伝学的機序の解析2012

    • Author(s)
      東出直樹
    • Organizer
      第52回日本呼吸器学会学術講演会
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] Whole-exome sequencing of familial non-small cell lung cancer patients with the EGFR gene mutations2012

    • Author(s)
      東出直樹
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] 東北大学病院におけるEML4ALK融合遺伝子陽2011

    • Author(s)
      小林誠、榊原智博、井上彰、福原達朗、貫和敏博
    • Organizer
      第52回日本肺癌学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2011-11-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 東北大学病院におけるEML4ALK 融合遺伝子陽

    • Author(s)
      小林誠、榊原智博、井上彰、福原達朗、貫和敏博
    • Organizer
      第52回日本肺癌学会総会
    • Place of Presentation
      大阪、日本
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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