Identification of the pirfenidone targeting molecule
Project/Area Number |
23790893
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Respiratory organ internal medicine
|
Research Institution | Tohoku University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 非閉塞性肺疾患 / 肺線維症 / ピルフェニドン |
Research Abstract |
To identify the pirfenidone targeting molecule, I conducted the affinity purification from culture cell lysates. RAW246.7, PC-9 and A549 culture cells were cultured to obtain the cytosolic and nuclear protein fractions. The affinity purification from RAW246.7lysate and subsequent SDS-PAGE and silver stain showed the candidates of the pirfenidone binding protein. But the repetitive experiment did not present the identical candidate proteins. The affinity purification from PC-9 lysate revealed the candidate of the pirfenidone binding protein. The pirfenidone targeting molecule could not be identified by the mass spectrometry due to the insufficiency of the amount of the candidate protein.
|
Report
(3 results)
Research Products
(12 results)