Differentiation into airway epithelial cells
| Project/Area Number |
23790899
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
AKIRA Saito 東京大学, 保健・健康推進本部, 助教 (90591412)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Keywords | 遺伝子導入 / 分化誘導 / 国際情報交換 / 遺伝子導入法 |
|---|
| Research Abstract |
In order to promote differentiation of human iPS (induced pluripotent stem) cells into lung epithelial cells, we tested the effects of various culture conditions, including inhibitors, growth factors and culture dish coating. We generated episomal vectors for TTF-1 (thyroid transcription factor-1), TAZ (transcriptional co-activator with PDZ-binding motif) and FOXA2 (forkhead box A2), and these genes were transduced into iPS cells or human skin fibroblasts by electroporation. Upregulation of markers for lung epithelial cells were examined by qRT-PCR (quantitative RT-PCR), and cell morphology of differentiated cells were investigated by electron microscopy.
|
Report
(4 results)
Research Products
(10 results)
-
-
-
-
-
[Journal Article] CD99 is a novel prognostic stromal marker in non-small cell lung cancer2012
Author(s)
Edlund K, Lindskog C, Saito A, Berglund A, Pontén F, Göransson-Kultima H, Isaksson A, Jirström K, Planck M, Johansson L, Lambe M, Holmberg L, Nyberg F, Ekman S, Bergqvist M, Landelius P, Lamberg K, Botling J, Ostman A, Micke P
-
Journal Title
Int J Cancer
Volume: 131(10)
Issue: 10
Pages: 2264-73
DOI
Related Report
Peer Reviewed
-
-
-
-
-
