Differentiation into airway epithelial cells

• Project/Area Number: 23790899
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: AKIRA Saito 東京大学, 保健・健康推進本部, 助教 (90591412)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 遺伝子導入 / 分化誘導 / 国際情報交換 / 遺伝子導入法

Research Abstract

In order to promote differentiation of human iPS (induced pluripotent stem) cells into lung epithelial cells, we tested the effects of various culture conditions, including inhibitors, growth factors and culture dish coating. We generated episomal vectors for TTF-1 (thyroid transcription factor-1), TAZ (transcriptional co-activator with PDZ-binding motif) and FOXA2 (forkhead box A2), and these genes were transduced into iPS cells or human skin fibroblasts by electroporation. Upregulation of markers for lung epithelial cells were examined by qRT-PCR (quantitative RT-PCR), and cell morphology of differentiated cells were investigated by electron microscopy.

Research Products

• [Journal Article] Histamine induces human lung fibroblast-mediated collagen gel contraction via histamine H1 receptor. (2014)
  • Author(s): 5.Horie M, Saito A, Yamauchi Y, Mikami Y, Sakamoto M, Jo T, Nakajima J, Takizawa H, Nagase T, Kohyama T.
  • Journal Title: Exp Lung Res. Volume: 40 Issue: 5 Pages: 222-36
  • DOI: 10.3109/01902148.2014.900155
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] An integrated expression profiling reveals target genes of TGF-βand TNF-αpossibly mediated by microRNAs in lung cancer cells (2013)
  • Author(s): Saito A, Suzuki HI, Horie M, Ohshima M, Morishita Y, Abiko Y, Nagase T
  • Journal Title: PLoS One Volume: 8(2) Issue: 1
  • DOI: 10.1371/annotation/874e0b87-0383-45ec-a250-3a4cd087dc86
  • Peer Reviewed
• [Journal Article] EMT and EndMT: regulated in similar ways? (2013)
  • Author(s): Akira Saito
  • Journal Title: Journal of Biochemistry Volume: 153(6) Issue: 6 Pages: 493-495
  • DOI: 10.1093/jb/mvt032
  • Peer Reviewed
• [Journal Article] An integrated expression profiling reveals target genes of TGF-β and TNF-α possibly mediated by microRNAs in lung cancer cells (2013)
  • Author(s): Akira Saito, Hiroshi I Iizuka, Masafumi Horie, Mitsuhiro Ohshima, Yasuyuki Morishita, Yoshimitsu Abiko, Takahide Nagase
  • Journal Title: PLoS ONE 8 Volume: 8 Issue: 1 Pages: 158-163
  • DOI: 10.1016/j.bbrc.2012.05.104
  • Peer Reviewed
• [Journal Article] CD99 is a novel prognostic stromal marker in non-small cell lung cancer (2012)
  • Author(s): Edlund K, Lindskog C, Saito A, Berglund A, Pontén F, Göransson-Kultima H, Isaksson A, Jirström K, Planck M, Johansson L, Lambe M, Holmberg L, Nyberg F, Ekman S, Bergqvist M, Landelius P, Lamberg K, Botling J, Ostman A, Micke P
  • Journal Title: Int J Cancer Volume: 131(10) Issue: 10 Pages: 2264-73
  • DOI: 10.1002/ijc.27518
  • Peer Reviewed
• [Journal Article] Characterization of human lung cancer-associated fibroblasts in three dimensional in vitro co-culture model (2012)
  • Author(s): Masafumi Horie, Akira Saito, Mitsuhiro Ohshima, Yasuyuki Morishita, Jun Nakajima, Tadashi Kohyama, Takahide Nagase
  • Journal Title: Biochem Biophys Res Commun Volume: 423 Issue: 2 Pages: 158-163
  • DOI: 10.1371/journal.pone.0056587
  • Peer Reviewed
• [Presentation] An integrated expression profiling reveals target genes of TGF-beta and TNF-alpha possibly mediated by microRNAs in lung cancer cells (2013)
  • Author(s): Akira Saito
  • Organizer: American Thoracic Society
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2013-05-20
• [Presentation] An integrated expression profiling reveals target genes of TGF-beta and TNF-alpha possibly mediated by microRNAs in lung cancer cells (2012)
  • Author(s): Akira Saito
  • Organizer: International Cancer Microenvironment Society
  • Place of Presentation: Suzhou, China
  • Year and Date: 2012-11-15
• [Presentation] An integrated expression profiling reveals target genes of TGF-β and TNF-αpossibly mediated by microRNAs in lung cancer cells (2012)
  • Author(s): 齋藤朗
  • Organizer: 2012 International Tumor Microenvironment Conference
  • Place of Presentation: Suzhou Dushu Lake Conference Center（中国・蘇州市）
• [Presentation] An Integrated Expression Profiling Reveals Target Genes Of TGF-β And TNF-α Possibly Mediated By microRNAs In Lung Cancer Cells
  • Author(s): Akira Saito
  • Organizer: American Thoracic Society 2013
  • Place of Presentation: Pennsylvania Convention Center(アメリカ・フィラデルフィア)