• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

MicroRNAs associated with smoking-induced pulmonary emphysema in senescence marker protein-30 knockout mice

Research Project

Project/Area Number 23790922
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Respiratory organ internal medicine
Research InstitutionJuntendo University

Principal Investigator

SATO Tadashi  順天堂大学, 医学部, 助教 (10596993)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords慢性閉塞性肺疾患(COPD) / microRNA / miR-146a / inflammation / smoking / SMP30 / マイクロRNA / 喫煙 / SMP30KOマウス / 炎症 / SMP30ノックアウトマウス / マイクロアレイ解析 / SMP30ノックアウトマウス
Research Abstract

Cigarette smoking is the most important cause of chronic obstructive pulmonary disease (COPD), but the mechanisms of pathogenesis are incompletely defined. We have previously reported that senescence marker protein-30 knockout (SMP30-KO) mice can be useful for investigating cigarette smoke-induced pulmonary emphysema. Moreover, we have already shown that miR-146a plays a pathogenetic role in the abnormal inflammatory response in COPD.
Vitamin C-controlled SMP30-KO were given miR-146a inhibitor on day 1 and 8 intranasally during exposure to either diluted cigarette smoke or fresh air for 2 weeks. Total cell and lymphocyte numbers were increased in the BALF of both miR-146a inhibitor and smoke exposed mice. PGE2 production and COX-2 expression were also increased significantly higher by exposure both miR-146a inhibitor and cigarette smoke.
In conclusion, miR-146a appears to play a pathogenetic role in the abnormal inflammatory response in a cigarette smoke-exposed murine model of COPD.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (16 results)

All 2014 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (6 results) Book (2 results)

  • [Journal Article] Vitamin C Prevents Cigarette Smoke-Induced Pulmonary Emphysema in Mice and Provides Pulmonary Restoration.2014

    • Author(s)
      Koike K, Ishigami A, Sato Y, Hirai T, Yuan Y, Kobayashi E, Tobino K, Sato T, Sekiya M, Takahashi K, Fukuchi Y, Maruyama N, Seyama K.
    • Journal Title

      Am J Respir Cell Mol Biol.

      Volume: 50 Issue: 2 Pages: 347-357

    • DOI

      10.1165/rcmb.2013-0121oc

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential Roles of JNK, ERK1/2, and p38 Mitogen-Activated Protein Kinases on Endothelial Cell Tissue Repair Functions in Response to Tumor Necrosis Factor-a.2013

    • Author(s)
      Kanaji N, Nelson A, Wang X, Sato T, Nakanishi M, Gunji Y, Basma H, Michalski J, Farid M, Rennard SI, Liu X.
    • Journal Title

      J Vasc Res.

      Volume: 50 Issue: 2 Pages: 145-156

    • DOI

      10.1159/000345525

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-inflammatory effects of budesonide in human lung fibroblasts are independent of histone deacetylase 2.2013

    • Author(s)
      Wang X, Nelson A, Weiler ZM, Patil A, Sato T, Kanaji N, Nakanishi M, Michalski J, Farid M, Basma H, LeVan TD, Miller-Lasson A, Wieslander E, Muller KC, Holz O, Magnussen H, Rabe KF, Liu X, Rennard SI.
    • Journal Title

      J Inflamm Res.

      Volume: 6 Pages: 109-119

    • DOI

      10.2147/jir.s43736

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prostaglandin E2 Stimulates the Production of Vasculaar Endothelial Growth Factor through the E-Prostanoid-2 Receptor in Cultured Human Lung Fibroblasts2012

    • Author(s)
      Nakanishi M, Sato T, Li Y, Nelson A, Farid M, Michalski J, Kanaji N, Wang X, Basma H, Patil A, Goraya J, Liu X, Togo S, Toews ML, Holz O, Muller K, Magnussen H, Rennard SI
    • Journal Title

      Am J Respir Cell Mol Biol

      Volume: 46 Issue: 2 Pages: 217-23

    • DOI

      10.1165/rcmb.2010-0115oc

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The p38 mitogen-activated protein kinases modulate endothelial cell survival and tissue repair2012

    • Author(s)
      Kanaji N, Nelson A, Allen-Gipson DS, Sato T, Nakanishi M, Wang X, Li Y, Basma H, Michalski J, Farid M, Rennard SI, Liu X
    • Journal Title

      Inflamm Res

      Volume: 61 Issue: 3 Pages: 233-11

    • DOI

      10.1007/s00011-011-0405-7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Attenuation of Inhibitory PGE2 Signaling in Human Lung Fibroblasts is Mediated by PDE4.2012

    • Author(s)
      佐藤 匡
    • Journal Title

      Am J Respir Cell Mol Biol

      Volume: 47 Issue: 6 Pages: 729-737

    • DOI

      10.1165/rcmb.2012-0057oc

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Interleukin-4 induces differentiation of human embryonic stem cells into "fibrogenic" fibroblast-like cells2011

    • Author(s)
      Sato T, Liu X, Basma H, Togo S, Sugiura H, Nelson A, Nakanishi M, Kanaji N, Wang X, Kim M, Li Y, Michalski J, Farid M, Sharp JG, Rennard SI
    • Journal Title

      J Allergy Clin Immun

      Volume: 127 Issue: 6 Pages: 1595-1603

    • DOI

      10.1016/j.jaci.2011.01.049

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inflammatory Cytokines Regulate Endothelial Cell Survival and Tissue Repair Functions via NF-kB Signaling2011

    • Author(s)
      Kanaji N, Sato T, Nelson A, Wang X, Li Y, Kim M, Nakanishi M, Basma H, Michalski J, Farid M, Chandler M, Pease W, Patil A, Rennard SI, Liu X
    • Journal Title

      J Inflamm Res

      Volume: 4 Pages: 127-38

    • DOI

      10.2147/jir.s19461

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] MicroRNAs associated with smoking-induced pulmonary emphysema in senescence marker protein-30 knockout mice2014

    • Author(s)
      佐藤匡
    • Organizer
      第52回日本呼吸器学会総会
    • Place of Presentation
      神戸
    • Year and Date
      2014-04-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 慢性閉塞性肺疾患(COPD)の発症と病態に関与するmicroRNA-SMP30ノックアウトマウスを用いた解析-2013

    • Author(s)
      佐藤匡
    • Organizer
      呼吸不全に関する調査研究班平成25年度第2回総会
    • Place of Presentation
      京都
    • Year and Date
      2013-12-20
    • Related Report
      2013 Final Research Report
  • [Presentation] MicroRNAs associated with smoking-induced pulmonary emphysema in senescence marker protein-30 knockout mice2012

    • Author(s)
      佐藤匡
    • Organizer
      American Thoracic Society International Conference 2012
    • Place of Presentation
      サンフランシスコ
    • Year and Date
      2012-05-22
    • Related Report
      2013 Final Research Report
  • [Presentation] MicroRNAs associated with smoking-induced pulmonary emphysema in senescence marker protein-30 knockout mice.2012

    • Author(s)
      佐藤 匡
    • Organizer
      第52回日本呼吸器学会総会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Presentation] MicroRNAs associated with smoking-induced pulmonary emphysema in senescence marker protein-30 knockout mice.2012

    • Author(s)
      佐藤 匡
    • Organizer
      American Thoracic Society International Conference 2012
    • Place of Presentation
      San Francisco
    • Related Report
      2012 Research-status Report
  • [Presentation] 慢性閉塞性肺疾患 (COPD)の発症と病態に関与するmicroRNA-SMP30ノックアウトマウスを用いた解析-.

    • Author(s)
      佐藤 匡
    • Organizer
      呼吸不全に関する調査研究班 平成25年度第2回総会.
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Book] Annual Review呼吸器2011

    • Author(s)
      佐藤匡, 他
    • Publisher
      中外医学社
    • Related Report
      2013 Final Research Report
  • [Book] 新しい診断と治療のABC(1)慢性閉塞性肺疾患

    • Author(s)
      佐藤匡, 他
    • Publisher
      最新医学社
    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi