| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
There was no significant change of the number of fibrocyte on fluorescence-activated cell sorter (FACS) analysis in peripheral blood monocytes from healthy control cultured with IL-13 or IL-4 for 5 days. CD14 positive mononuclear cells selected by magnetic beads from PBMCs of healthy control and patients with nonspecific interstitial pneumonia were cultured with IL-13 or IL-4, and the number of the cultured cells and analysis of polymerase chain reaction (PCR) of CD14 and S100A4 of cell lysate from cultured cells did not significantly changed from non-stimulated cells. U937 cells were cultured with IL-13 and IL-4, however there was no significant change of the number of fibrocyte on FACS analysis. Analysis of PCR of cell lysate from cultured U937 cells and cultured spleen cells from bleomycin induced fibrosis model of mice with IL-13 did not significantly changed compared with non-stimulated cells.
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