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Effect of TGF-β on WT1 expression in podocyte.

Research Project

Project/Area Number 23790930
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Kidney internal medicine
Research InstitutionGunma University

Principal Investigator

SAKAIRI Toru  群馬大学, 医学部, 助教 (20455976)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords糖尿病性腎症 / 巣状糸球体硬化症 / 糸球体上皮細胞 / TGF-beta1 / WT1 / TGF-beta / Wilms tumor 1 / TGF-beta 1 / podocyte
Research Abstract

TGF-beta1 is involved in diabetes nephropathy and focal segmental glomerulonephritis. Transcriptional factor Wilms tumor suppressor gene (WT1) is essential for normal function of podocytes. We found that TGF-beta1 reduces WT1 expression in both cultured humanpodocytes and mouse podocytes. Using the cultured podocytes, we identified following mechanisms by which TGF-beta1 suppress WT1 expression. First, Smad4 mediates a decrease in WT1 expression by TGF-beta1. Second, TGF-beta1 caused DNA methylation in promoter region of the podocytes. Our results indicate that diverse pathways contribute to the TGF-beta1-induced WT1 reduction that may result in podocyte injury.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2011 2010 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (11 results) Remarks (2 results)

  • [Journal Article] TGF-beta1 reduces Wilms' tumor suppressor gene expression in podocytes2011

    • Author(s)
      Sakairi T, Abe Y, Kopp JB
    • Journal Title

      Nephrol DialTransplant

      Volume: 26(9) Pages: 2746-52

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] TGF-beta1 reduces Wilms' tumor suppressor gene expression in podocytes.2011

    • Author(s)
      Sakairi T, Abe Y, Kopp JB.
    • Journal Title

      Nephrol Dial Transplant

      Volume: 26 Issue: 9 Pages: 2746-2752

    • DOI

      10.1093/ndt/gfr061

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] miR-143 contributes to podocyte injury induced by TGF-betal2011

    • Author(s)
      Sakairi T, Abe Y, Hiromura K, TakahashiS, Hamatani H, Nojima Y, and Kopp JB
    • Organizer
      The 44th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Philadelphia, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Transforming Growth Factor (TGF)-β1 Induced DNA Methylation of Wilms' Tumor Suppressor Gene (WT1) Promoter in Human Podocytes2011

    • Author(s)
      Hamatani H, Hiromura K, Sakairi T, Takahashi S, Ikeuchi H, Maeshima A, and Nojima Y
    • Organizer
      The 44th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Philadelphia, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] miR-143 contributes to podocyte injury induced by TGF-beta12011

    • Author(s)
      Sakairi T, Abe Y, Hiromura K, Takahashi S, Hamatani H, Nojima Y, and Kopp JB
    • Organizer
      The 44th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Philadelphia, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Transforming Growth Factor (TGF)-β1 Induced DNA Methylation of Wilms’ Tumor Suppressor Gene (WT1) Promoter in Human Podocytes.2011

    • Author(s)
      Hamatani H, Hiromura K, Sakairi T, Takahashi S, Ikeuchi H, Maeshima A, Nojima Y.
    • Organizer
      The 44th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Philadelphia, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] A Novel Case of Nephrotic Syndrome Caused by Immune-Mediated Severe LCAT Deficiency.2011

    • Author(s)
      Takahashi S, Hiromura K, Tsukida M, Ohishi Y, Hamatani H, Sakurai N, Sakairi T, Ikeuchi H, Maeshima A, Kuroiwa T, Nagata M, and Nojima Y.
    • Organizer
      The 44th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Philadelphia, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] TGF-beta1 は、糸球体上皮細胞におけるWT1の発現を抑制する2011

    • Author(s)
      坂入 徹、阿部 祥英、廣村 桂樹、高橋 哲史、浜谷 博子、櫻井 則之、池内 秀和、野島 美久、Kopp Jeffrey
    • Organizer
      第54回日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] SIRPαシグナル経路の遮断は糖尿病性腎症を増悪させる2011

    • Author(s)
      高橋 哲史1、廣村 桂樹1、浜谷 博子1、加藤 麻衣1、坂入 徹、櫻井 則之、池内 秀和、前嶋 明人、黒岩 卓、青木 武生、大西 浩史、的崎 尚、野島 美久
    • Organizer
      第54回日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] 後天性LCAT欠損によるネフローゼ症候群の1例とその機序解析2011

    • Author(s)
      高橋 哲史、廣村 桂樹、月田 真祐子、大石 裕子、浜谷 博子、櫻井 則之、坂入 徹、池内 秀和、前嶋 明人、黒岩 卓、長田 道夫、野島 美久
    • Organizer
      第54回日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] ループス腎炎の寛解導入における、タクロリムス(TAC)とミコフェノール酸モフェチル(MMF)の併用療法の試み2011

    • Author(s)
      池内 秀和、廣村 桂樹、高橋 哲史、月田 真祐子、内山 和彦、櫻井 則之、坂入 徹、前嶋 明人、黒岩 卓、野島 美久
    • Organizer
      第54回日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] 複数のThymidineアナログを用いたlabel-retaining cells(LRCs)の検討2011

    • Author(s)
      前嶋 明人、宮 政明、三島 敬一郎、櫻井 則之、池内 秀和、坂入 徹、黒岩 卓、廣村 桂樹、野島 美久
    • Organizer
      第54回日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] TGF-beta reduces Wilms tumor 1 expression in podocytes in vitro and in vivo2010

    • Author(s)
      Sakairi T, Abe Y, and Kopp JB
    • Organizer
      The 43th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Denver, USA
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://mcs.dept.med.gunma-u.ac.jp/index.html

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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