The role of thrombin cleaved osteopontin in ischemic reperfusion renal injury models

• Project/Area Number: 23790945
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Ehime University
• Principal Investigator: IRITA Jun 愛媛大学, 医学部附属病院, 講師(病院教員) (00423442)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 急性腎障害 / オステオポンチン / トロンビン切断型オステオポンチン

Research Abstract

Osteopontin (OPN) has been implicated in the pathology of several renal conditions. The aim of the present study was to clarify the roles of OPN in acute renal injury into either wild type (WT) or OPN knockout mice (OPN-/-). We used ischemia-reperfusion models to exaggerate acute renal injury. We demonstrated for the first time that thrombin cleaved OPN was expressed in acute kidney injury models. These results indicate OPN is a promoter of ischemia-induced apoptosis in the kidney and suggest that inhibition of OPN may be a potential therapeutic target for prevention of acute renal injury.

Research Products

• [Journal Article] Relationship between Renal Hemodynamics and Urinary Type IV Collagen in Patients with Essential Hypertension (2012)
  • Author(s): nomoto D, Okura T, Nagao T, Jotoku M, Irita J, Miyoshi KI, Higaki J
  • Journal Title: Clin Exp Hypertens
  • Peer Reviewed
• [Journal Article] Osteopontin plays a critical role in interstitial fibrosis but not glomerular sclerosis in diabetic nephropathy (2012)
  • Author(s): Nagao T, Okura T, Irita J, Jotoku M, Enomoto D, Desilva VR, Miyoshi K, Kurata M, Matsui Y, Uede T, Higaki J
  • Journal Title: Nephron Extra Volume: 2 Pages: 87-103
  • Peer Reviewed
• [Journal Article] Osteopontin deficiency protects against aldosterone-induced inflammation, oxidative stress, and interstitial fibrosis in the kidney (2011)
  • Author(s): Irita J, Okura T, Jotoku M, Nagao T, Enomoto D, Kurata M, Desilva VR, Miyoshi KI, Matsui Y, Uede T, Denhardt DT, Rittiling SR, Higaki J
  • Journal Title: Am J Physiol Renal Physiol Volume: 301
  • Peer Reviewed
• [Journal Article] Angiotensin II receptor blockade with valsartan decreases plasma osteopontin levels in patients with essential hypertension (2011)
  • Author(s): Kurata M, Okura T, Irita J, Enomoto D, Nagao T, Jotoku M, Miyoshi KI, Desilva VR, Higaki J
  • Journal Title: J Hum Hypertens Volume: 25 Pages: 333-339
  • Peer Reviewed
• [Presentation] Osteopontin Deficiency Attenuates Aldosterone induced Tubulointerstitial Fibrosis, EMT, and Oxidative Stress. (2011)
  • Author(s): Jun Irita, Takafumi Okura, Masanori Jotoku, Tomoaki Nagao, Daijiro Enomoto, Ken-ichi Miyoshi, Jitsuo Higaki.
  • Organizer: The 13th China-Japan Joint Hypertension Symposium
  • Place of Presentation: Beijing, China.
  • Year and Date: 2011-12-04
• [Presentation] アルドステロンによる尿細管間質線維化発症機序-オステオポンチンと EMT 及び酸化ストレスの関連について (2011)
  • Author(s): 入田 純、大蔵隆文、長尾知明、城徳昌典、榎本大次郎、三好賢一、檜垣實男
  • Organizer: 第34回日本高血圧学会総会
  • Place of Presentation: 宇都宮
• [Remarks]
  • URL: http://www.m.ehime-u.ac.jp/school/int.med2/