Elucidation of Pathogenic mechanisms for glomerulonephritis and therapeutic application by a newly identified dendritic cell subset

• Project/Area Number: 23790962
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Nippon Medical School
• Principal Investigator: NAGASAKA Shinya 日本医科大学, 医学部, 助教 (00573239)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 新規樹状細胞サブセット / 糸球体腎炎 / プロスタグランジンE2 / Th17 / 免疫学 / 樹状細胞 / 腎炎 / 炎症

Research Abstract

First, I showed that in the point of view of decrease of IL-12p70 production, upregulation of IL-23 production, and enhanced expression of DC maturation markers, new DC subset which I previously identified had very similar characteristics to DC differentiated under prostaglandin E2 (PGE2).Next, I focused on interaction of PGE2 on DC. I showed that PGE2 interacted with renal-DC which had been considered to have important role for glomerulonephritis, and enhanced productions of pro-inflammatory cytokines for example IL-6.The present study suggested that the DC-PGE2 interaction would be involved in pathogenic and progression mechanism of glomerulonephritis.

Research Products

• [Journal Article] Renal inflammatory changes in acute hepatic failure-associated acute kidney injury. (2013)
  • Author(s): Shimizu A, Ishii E, Masuda Y, Sato A, Piao H, Kunugi S, Takahashi M, Terasaki M, Nagasaka S, Terasaki Y, Ohashi R, Morioka T, Fukuda Y.
  • Journal Title: Am J Nephrol. Volume: 37 Issue: 4 Pages: 378-88
  • DOI: 10.1159/000348567
  • Peer Reviewed
• [Journal Article] Surgical technique of orthotopic liver transplantation in rats: the Kamada technique and a new splint technique for hepatic artery reconstruction. (2013)
  • Author(s): Ishii E, Shimizu A, Takahashi M, Terasaki M, Kunugi S, Nagasaka S, Terasaki Y, Ohashi R, Masuda Y, Fukuda Y.
  • Journal Title: J Nippon Med Sch. Volume: 80(1) Pages: 4-15
  • NAID: 130004147203
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/23470801
  • Peer Reviewed
• [Journal Article] Proliferative glomerulonephritis with monoclonal immunoglobulin G3κ deposits in association with parvovirus B19 infection. (2012)
  • Author(s): Fujita E, Shimizu A, Kaneko T, Masuda Y, Ishihara C, Mii A, Higo S, Kajimoto Y, Kanzaki G, Nagasaka S, Iino Y, Katayama Y, Fukuda Y.
  • Journal Title: Hum Pathol. Volume: 43(12) Issue: 12 Pages: 2326-33
  • DOI: 10.1016/j.humpath.2012.04.004
  • Peer Reviewed
• [Journal Article] Inhibition of matrix metalloproteinases reduces ischemia-reperfusion acute kidney injury. (2011)
  • Author(s): Kunugi S, Shimizu A, Kuwahara N, Du X, Takahashi M, Terasaki Y, Fujita E, Mii A, Nagasaka S, Akimoto T, Masuda Y, Fukuda Y.
  • Journal Title: Lab Invest. Volume: 91(2) Issue: 2 Pages: 170-80
  • DOI: 10.1038/labinvest.2010.174
  • Peer Reviewed
• [Presentation] スタチンはマクロファージ分化・活性化を直接抑制し、抗炎症機能を誘導する (2012)
  • Author(s): 永坂真也
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Statin directly inhibit macrophage differentiation and activation, and provide anti0inflammatory function (2012)
  • Author(s): Shinya Nagasaka
  • Organizer: American Society of Nephrology Kidney Week2012
  • Place of Presentation: San Diego, USA
• [Presentation] スタチン存在下で分化させたマクロファージはCox-2発現が増強する (2012)
  • Author(s): 永坂真也
  • Organizer: 第55回日本腎臓学会学術総会
  • Place of Presentation: 横浜
• [Presentation] スタチン処置マクロファージを用いた糸球体腎炎抑制効果の検討 (2012)
  • Author(s): 永坂真也
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] Statin directly inhibit macrophage differentiation and activation, and provide anti-inflammatory function. (2012)
  • Author(s): Shinya Nagasaka
  • Organizer: American Society of Nephrology Kidney Week 2012
  • Place of Presentation: San Diego, USA
• [Presentation] スタチン存在下で分化させたマクロファージはCox-2発現が増強する (2012)
  • Author(s): 永坂真也
  • Organizer: 第55回日本腎臓学会学術集会
  • Place of Presentation: 横浜
• [Presentation] スタチン処理マクロファージを用いた糸球体腎炎抑制効果の検討 (2012)
  • Author(s): 永坂真也
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] スタチン処理マクロファージによる糸球体腎炎に対する抗炎症効果 (2011)
  • Author(s): 永坂真也
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] スタチン（Atorvastatin）によるマクロファージを介した抗炎症機構の解析 (2011)
  • Author(s): 永坂真也、清水章、藤田恵美子、寺崎美佳、高橋美紀子、功刀しのぶ、寺崎泰弘,、益田幸成、福田悠
  • Organizer: 第54回日本腎臓学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] スタチン処理マクロファージによる糸球体腎炎に対する抗炎症効果 (2011)
  • Author(s): 永坂真也、清水章、益田幸成、寺崎泰弘、寺崎美佳、高橋美紀子、功刀しのぶ、福田悠
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Presentation] スタチン(Atorvastatin)によるマクロファージを介した抗炎症機構の解析
  • Author(s): 永坂真也
  • Organizer: 第54回日本腎臓学会学術総会
  • Place of Presentation: 横浜