Therapeutic effects of isoquinoline alkaloids isolated from Coptis Rhizoma, potential anti-glomerulonephritis

• Project/Area Number: 23790966
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Aichi Gakuin University
• Principal Investigator: SUZUKI Hiroka 愛知学院大学, 薬学部, 助教 (00581026)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 糸球体腎炎 / 黄連含有イソキノリンアルカロイド / TRPA1 / casein kinase 2

Research Abstract

Effects of isoquinoline alkaloids isolated from Coptis Rhizoma were examined on activity of Transient receptor potential（TRP）channel, which has multiple physiological functions in biological organs. Furthermore, stimulatory effects of various drugs including auranofin and some NADPH oxidase inhibitors on TRP ankyrin1（TRPA1） channel were identified.

Research Products

• [Journal Article] TRPV4 partially participates in proliferation of human brain capillary endothelial cells. (2013)
  • Author(s): Noriyuki Hatano, Hiroka Suzuki, Yuka Itoh, Katsuhiko Muraki.
  • Journal Title: Life Sciences Volume: Vol.92、No.4-5 Issue: 4-5 Pages: 317-324
  • DOI: 10.1016/j.lfs.2013.01.002
  • Peer Reviewed
• [Journal Article] Stimulation of human TRPA1 channels by clinical concentrations of theanti-rheumatic drug, auranofin. (2013)
  • Author(s): Noriyuki Hatano, Hiroka Suzuki, YukikoMuraki, Katsuhiko Muraki.
  • Journal Title: American Journal of Physiology - Cell Physiology Volume: Vol.304、No.2 Pages: 354-361
  • Peer Reviewed
• [Journal Article] Stimulation of human TRPA1 channels by clinical concentrations of the anti-rheumatic drug, auranofin. (2013)
  • Author(s): Noriyuki Hatano
  • Journal Title: Cell Physiology: American Journal of Physiology Volume: 304
  • Peer Reviewed
• [Journal Article] HIF1α switches on TRPA1 gene expression via a hypoxia response element-like motif to modulate cytokine release (2012)
  • Author(s): N Hatano, Y Itoh, H Suzuki, Y Muraki, H Hayashi, K Onozaki, IC Wood, DJ Beech & K Muraki
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 38 Pages: 31962-31972
  • DOI: 10.1074/jbc.m112.361139
  • Peer Reviewed
• [Journal Article] Differential gene expression in rat vascular smooth muscle cells following treatment with coptisine exerts aselective antiproliferative effect. (2012)
  • Author(s): Hiroka Suzuki, Horoki Tanabe, HajimeMizukami, Makoto Inoue.
  • Journal Title: Journal of Natural Products Volume: Vol.74、No.4 Issue: 4 Pages: 634-638
  • DOI: 10.1021/np100645d
  • Peer Reviewed
• [Journal Article] Differential gene expression in rat vascular smooth muscle cells following treatment with coptisine exerts a selective antiproliferative effect. (2011)
  • Author(s): Suzuki H, Tanabe H, Mizukami H, Inoue M.
  • Journal Title: J Nat Prod. Volume: 74(4) Pages: 634-638
  • Peer Reviewed
• [Presentation] NADPHオキシダーゼ阻害剤はTRPA1チャネルを活性化する (2013)
  • Author(s): 鈴木裕可,波多野紀行,村木由起子,伊藤友香,林秀敏,小野嵜菊夫,村木克彦
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-29
• [Presentation] Potent activation of TRPA1 channel byNADPH oxidase inhibitors (2013)
  • Author(s): Hiroka Suzuki, Noriyuki Hatano, Yukiko Muraki, Yuka Itoh, Hidetoshi Hayashi, Kikuo Onozaki, Katsuhiko Muraki.
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2013-03-21
• [Presentation] Potent activation of TRPA1 channel by NADPH oxidase inhibitors. (2013)
  • Author(s): Hiroka Suzuki
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡
• [Presentation] 抗リウマチ薬オーラノフィンはTRPA1チャネルを活性化する (2012)
  • Author(s): 鈴木裕可,楠神枝里香,神谷絵梨奈,藤嵩久,波多野紀行,村木由起子,村木克彦
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-29
• [Presentation] Selective and potent activation of human TRPA1 by a disease modifying anti-rheumatic drug, auranofin (2012)
  • Author(s): Noriyuki Hatano, Erika Kusugami, Erina Kamiya, Hiroka Suzuki, Yukiko Muraki, Katsuhiko Muraki.
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-16