Analysis of neuroprotective function of Parkinson's Disease Gene DJ-1

• Project/Area Number: 23790970
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Hokkaido University
• Principal Investigator: TAKAHASHI-NIKI Kazuko 北海道大学, 薬学研究科(研究院), 助教 (50447645)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: DJ-1 / パーキンソン病 / PARK7 / 酸化ストレス / 分泌

Research Abstract

[Function analysis of DJ-1 secreted under oxidative stress] We found that secreted DJ-1 from astrocytes protected co-cultured neurons against oxidative stress. Furthermore, we found that the amount of secreted DJ-1 mutants found in Familial Parkinson's disease PARK7 was less than DJ-1 wt.
[Function analysis of DJ-1 as protease] We analyzed the biochemical characterization of DJ-1 protease. Futhermore, we identified the recognition sequences for DJ-1 protease and targeted proteins.

Research Products

• [Journal Article] Transcriptional activation of the cholecystokinin gene by DJ-1 through interaction of DJ-1 with RREB1 and the effect of DJ-1 on the cholecystokinin level in mice (2013)
  • Author(s): Yamane, T., Suzui, S., Kitaura, H., Takahashi-Niki K,, Iguchi-Ariga, S.M.M. and Ariga, H.
  • Journal Title: PLoS ONE Volume: 8(11)
  • Peer Reviewed
• [Journal Article] Identification of the recognition sequence and target proteins for DJ-1 protease (2013)
  • Author(s): Mitsugi H., Niki T., Takahashi-Niki K, Tanimura K., Yoshizawa-Kumagaye K, Tsunemi M.,Iguchi-Ariga SM, Ariga H.
  • Journal Title: FEBS Letters Volume: 587(16) Issue: 16 Pages: 2493-9
  • DOI: 10.1016/j.febslet.2013.06.032
  • Peer Reviewed
• [Journal Article] Neuroprotective Function of DJ-1 against Parkinson's Disease (2013)
  • Author(s): Ariga H. Takahashi-Niki K, Kato I, Maita H, Niki T,, Iguchi-Ariga SM
  • Journal Title: SM Oxidative Medicine and Cellular Longevity Volume: 683920 Pages: 683920-683920
  • DOI: 10.1155/2013/683920
  • Peer Reviewed
• [Journal Article] Oxidized DJ-1 inhibits p53 by sequestering p53 from promoters in a DNA-binding affinity-dependent manner. (2013)
  • Author(s): Kato, I.
  • Journal Title: Mol. Cell. Biol. Volume: 33 Issue: 2 Pages: 340-359
  • DOI: 10.1128/mcb.01350-12
  • Peer Reviewed
• [Journal Article] Neuroprotective function of DJ-1 in Parkinson's disease. (2013)
  • Author(s): Ariga H, Takahashi-Niki K, Kato I, Maita H, Niki T, Iguchi-Ariga SM.
  • Journal Title: Oxid Med Cell Longev. Volume: 2013
  • Peer Reviewed
• [Journal Article] Identification of the recognition sequence and target proteins for DJ-1 protease. (2013)
  • Author(s): Mitsugi H, Niki T, Takahashi-Niki K, Tanimura K, Yoshizawa-Kumagaye K, Tsunemi M, Iguchi-Ariga SM, Ariga H.
  • Journal Title: FEBS Lett. Volume: 587
  • Peer Reviewed
• [Journal Article] Transcriptional activation of the cholecystokinin gene by DJ-1 through interaction of DJ-1 with RREB1 and the effect of DJ-1 on the cholecystokinin level in mice. (2013)
  • Author(s): Yamane T, Suzui S, Kitaura H, Takahashi-Niki K, Iguchi-Ariga SM, Ariga H.
  • Journal Title: PLoS One. Volume: 8
  • Peer Reviewed
• [Journal Article] Transcriptional activation of low-density lipoprotein receptor gene by DJ-1 and effect of DJ-1 on cholesterol homeostasis (2012)
  • Author(s): Yamaguchi, S., Yamane, T., Takahashi-Niki, K., Kato, I., Niki, T., Goldberg, M. S., Shen, J., Ishimoto, K., Doi, T., Iguchi-Ariga, S. M. M., Ariga, H.
  • Journal Title: PLoS ONE Volume: 7 Issue: 5 Pages: e38144-e38144
  • DOI: 10.1371/journal.pone.0038144
  • Peer Reviewed
• [Journal Article] Stimulation of vesicular monoamine transporter 2 activity by DJ-1 in SH-SY5Y cells (2012)
  • Author(s): Ishikawa, S., Tanaka, Y., Takahashi-Niki, K., Niki, T., Ariga, H., Iguchi-Ariga, S. M. M.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 42 Issue: 4 Pages: 813-818
  • DOI: 10.1016/j.bbrc.2012.04.095
  • Peer Reviewed
• [Journal Article] Function of DJ-1 in mitochondria (2012)
  • Author(s): Takahashi-Niki K, Niki T, Iguchi-Ariga S, Ariga H.
  • Journal Title: Yakugaku Zasshi. Volume: 132 Pages: 1105-1110
• [Journal Article] Transcriptional activation of low-density lipoprotein receptor gene by DJ-1 and effect of DJ-1 on cholesterol homeostasis. (2012)
  • Author(s): Yamaguchi S, Yamane T, Takahashi-Niki K, Kato I, Niki T, Goldberg MS, Shen J, Ishimoto K, Doi T, Iguchi-Ariga SM, Ariga H.
  • Journal Title: PLoS One. Volume: 7
  • Peer Reviewed
• [Journal Article] Stimulation of vesicular monoamine transporter 2 activity by DJ-1 in SH-SY5Y cells. (2012)
  • Author(s): Ishikawa S, Tanaka Y, Takahashi-Niki K, Niki T, Ariga H, Iguchi-Ariga SM.
  • Journal Title: Biochem Biophys Res Commun Volume: 421 Pages: 813-818
• [Journal Article] Neuroprotective effect of a new DJ-1-binding compound against neurodegeneration in Parkinson's disease and stroke model rats (2011)
  • Author(s): Kitamura, Y., Watanabe, S., Taguchi, M., Takagi, K., Kawata, T., Takahashi-Niki, K., Yasui, H., Maita, H., Iguchi-Ariga, S. M. M., Ariga, H.
  • Journal Title: Mol. Neurodegeneration Volume: 6 Issue: 1 Pages: 48-48
  • DOI: 10.1186/1750-1326-6-48
  • Peer Reviewed
• [Journal Article] Neuroprotective effect of a new DJ-1-binding compound against neurodegeneration in Parkinson's disease and stroke model rats. (2011)
  • Author(s): Kitamura Y, Watanabe S, Taguchi M, Takagi K, Kawata T, Takahashi-Niki K, et al.
  • Journal Title: Mol Neurodegener. 2011 Volume: 6 Pages: 48-66
  • Peer Reviewed
• [Presentation] アストロサイトから分泌されたDJ-1による神経細胞死抑制 (2013)
  • Author(s): 仁木(高橋)加寿子、綿引佑実、仁木剛史、北浦廣剛、有賀(井口)早苗、有賀寛芳
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2013-09-11
• [Presentation] アストロサイトから分泌されたDJ-1による神経細胞死抑制 (2013)
  • Author(s): 仁木（高橋）加寿子、綿引祐実、仁木剛史、北浦廣剛、有賀（井口）早苗、有賀寛芳
  • Organizer: 日本生化学会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] DJ-1によるLDLR転写活性化およびコレステロール恒常性維持 (2013)
  • Author(s): 仁木（高橋）加寿子、仁木剛史、有賀（井口）早苗、有賀寛芳
  • Organizer: 第14回 Pharmaco-Hematology シンポジウム
  • Place of Presentation: 長井記念ホール（東京都）
  • Invited
• [Presentation] パーキンソン病原因遺伝子DJ-1の抗酸化ストレス機能の解析 (2012)
  • Author(s): 仁木(高橋)加寿子、仁木剛史、有賀(井口)早苗、有賀寛芳
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2012-12-15
• [Presentation] DJ-1のミトコンドリアにおける機能 (2012)
  • Author(s): 仁木(高橋)加寿子、仁木剛史、米田千夏、我那覇陽子、有賀(井口)早苗、有賀寛芳
  • Organizer: 日本薬学会第132回年会シンポジウム
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2012-03-29
• [Presentation] DJ-1のミトコンドリアにおける機能 (2012)
  • Author(s): 仁木(高橋)加寿子、仁木剛史、米田千夏、我那覇陽子、有賀(井口)早苗、有賀寛芳
  • Organizer: ミトコンドリアとDDS2
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2012-01-20
• [Presentation] パーキンソン病原因遺伝子DJ-1の抗酸化ストレス機能の解析 (2012)
  • Author(s): 仁木（高橋） 加寿子、仁木剛史、有賀（井口）早苗、有賀 寛芳
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] DJ-1のミトコンドリアにおける機能 (2012)
  • Author(s): 仁木 加寿子
  • Organizer: ミトコンドリアとDDS2(招待講演)
  • Place of Presentation: 北海道大学（札幌市）
• [Presentation] DJ-1のミトコンドリアにおける機能 (2012)
  • Author(s): 仁木 加寿子
  • Organizer: 日本薬学会 第132年会
  • Place of Presentation: 北海道大学（札幌市）
• [Presentation] DJ-1の抗酸化ストレスセンサーとしての役割 (2011)
  • Author(s): 仁木(高橋)加寿子、仁木剛史、有賀(井口)早苗、有賀寛芳
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2011-09-22
• [Presentation] Oxidative stress-response by DJ-1 and its binding compounds (2011)
  • Author(s): Takahashi-Niki K, Niki, T, Iguchi-Ariga, S.M.M and Ariga, H
  • Organizer: ISN-ESN 2011 23rd Biennial Meeting
  • Place of Presentation: ギリシャアテネ
  • Year and Date: 2011-08-29
• [Presentation] Role of DJ-1 as an anti-oxidative stress sensor (2011)
  • Author(s): Takahashi-Niki K
  • Organizer: ISN-ESN-2011
  • Place of Presentation: Megaron Athens International Conference Centre (ギリシャ)
• [Presentation] DJ-1の抗酸化ストレスセンサーとしての役割 (2011)
  • Author(s): 仁木 加寿子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館（京都府）
• [Remarks]
  • URL: http://bunseiserver.pharm.hokudai.ac.jp/
• [Remarks] 北海道大学薬学部分子生物学分野へようこそ
  • URL: http://bunseiserver.pharm.hokudai.ac.jp/