Understanding of spontaneous generation of prions in sporadicCreutzfeld-Jakob disease
| Project/Area Number |
23790998
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurology
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| Research Institution | Nagasaki University |
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Principal Investigator |
SANO Kazunori 長崎大学, 大学院・医歯薬学総合研究科, 助教 (50534343)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 神経分子病態学 / プリオン / 孤発性クロイツフェルトヤコブ病 / dsRNA / 感染 |
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| Research Abstract |
Prion diseases are infectious and fatal neurodegenerative disorders characterized by progressive spongiform changes and the accumulation of abnormal prion protein (PrPSc) in the central nervous system. Although prion diseases were thought to be caused by slow-virus infections, no exogenous viral genome has been identified. The infectious agent, now called prion, is thought not to possess its own genome and to be composed uniquely of prion proteins, which are encoded by the host gene. In this study, we found that polyinosine-polycytidylic acid (poly(I:C)), a synthetic analog of dsRNA,-treated mice increased accumulation of PrPScin prion-infected cells, and accelerated the onset of prion disease in wild-type mice. Moreover, PrPScwas increased by overexpression of Toll-like receptor 3 (TLR3), Retinoic acid-inducible gene-I (RIG-I), and Melanoma differentiation-associated protein 5 (MDA5), which recognize dsRNA, in prion-infected cells. Our findings suggest that dsRNA may play a key role in prion infection.
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Report
(3 results)
Research Products
(3 results)
