Research Project
Grant-in-Aid for Young Scientists (B)
Parkin is a neuroprotective protein with many functions, including maintaining mitochondrial homeostasis. Recent evidence suggests that Parkin is recruited from the cytoplasm to damaged mitochondria with low membrane potential. We found that intracellular localization of Parkin changed with cellular growth phase. Parkin was preferentially localized in the mitochondria of cultured cells in the late log phase of growth. The mitochondria with large amounts of Parkin showed preserved membrane potentials even during treatment with carbonyl cyanide m-chlorophenylhydrazone. Here we report a novel protein named Klokin 1 that transports Parkin to the mitochondria. Klokin 1 was localized to the mitochondria and enhanced mitochondrial expression of Parkin. Klokin 1 enhanced cell viability in Parkin-silenced cells. Klokin 1 expression was enhanced in the brains of Parkin-deficient mice but not in an autopsied PARK2 brain. Our findings indicate that mitochondrial Parkin prevents mitochondrial depolarization and cellular apoptosis and that Klokin 1 may compensate for Parkin deficiency.
All 2012 2011 Other
All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (4 results) Remarks (1 results)
Hum Mol Genet.
Volume: 21(5) Pages: 991-1003
Human Molecular Genetics
Volume: Vol.21 Issue: 5 Pages: 991-1003
10.1093/hmg/ddr530
J Tokushima Natl Hosp
Volume: 2 Pages: 32-35
40019066369
Volume: 2 Pages: 23-26
40019066348
Acta Neurol Belg.
Volume: 111 Pages: 188-194
Arch Med Sci
Volume: (in press)
http://www.tokusimahosp-nho.jp/
