Analysis of adipocyte-specific transcriptional gene regulation by using next generation sequencer
| Project/Area Number |
23791020
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Metabolomics
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
WAKI Hironori 東京大学, 医学部附属病院, 特任准教授 (00466765)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 分化 / 遺伝子 / ゲノム / 糖尿病 / 脂肪細胞 / FAIRE / クロマチン / PPARg / 転写 / クロマチン免疫沈降 / 次世代シークエンサー / NFI |
|---|
| Research Abstract |
We generated genome-wide maps of open chromatin, histone modifications and PPARγ binding sites in 3T3-L1 adipocytes using FAIRE-seq and ChIP-seq. The data suggest that regulatory elements located distal to target genes play a critical role in adipocyte-specific gene regulation. We also demonstrated that a combination of motif analysis and FAIRE-seq provides unbiased identification of regulators of adipocyte-specific gene expression and reported identification of the NFI transcription family as a novel regulator of adipocyte differentiation.
|
Report
(3 results)
Research Products
(29 results)
-
-
[Journal Article] Global Mapping of Cell Type-Specific Open Chromatin by FAIRE-seq Reveals the Regulatory Role of the NFI Family in Adipocyte Differentiation2011
Author(s)
Hironori Waki, Masahiro Nakamura, Toshimasa Yamauchi, Ken-ichi Wakabayashi, Jing Yu, Lisa Hirose-Yotsuya, Kazumi Take, Wei Sun, Masato Iwabu, Miki Okada-Iwabu, Takanori Fujita, Tomohisa Aoyama, Shuichi Tsutsumi, Kohjiro Ueki, Tatsuhiko Kodama, Juro Sakai, Hiroyuki Aburatani, Takashi Kadowaki
-
Journal Title
Related Report
Peer Reviewed
-
[Journal Article] Global mapping of cell type-specific open chromatin by FAIRE-seq reveals the regulatory role of the NFI family in adipocyte differentiation2011
Author(s)
Waki H, Nakamura M, Yamauchi T, Wakabayashi K, Yu J, Hirose-Yotsuya L, Take K, Sun W, Iwabu M, Okada-Iwabu M, Fujita T, Aoyama T, Tsutsumi S, Ueki K, Kodama T, Sakai J, Aburatani H, Kadowaki
-
Journal Title
PLos Genetics
Volume: 7
Issue: 10
Pages: e1002311-e1002311
DOI
Related Report
Peer Reviewed
-
-
-
-
[Presentation] Global Mapping of Cell-Type-Specific Open Chromatin by FAIRE-seq Reveals the Regulatory Role of the NFI Family in Adipocyte Differentiation2012
Author(s)
Hironori Waki, Masahiro Nakamura, Toshimasa Yamauchi, , Ken-ichi Wakabayashi, Jing Yu, Lisa Hirose Yotsuya, Kazumi Take, Wei Sun, Masato Iwabu, Miki Okada-Iwabu, , Takanori Fujita, Tomohisa Aoyama, Shuichi Tsutsumi, Kohjiro Ueki, Tatsuhiko Kodama, Juro Sakai, Hiroyuki Aburatani, and Takashi Kadowaki
Organizer
Keystone Symposia Genetic and Molecular Basis of Obesity and Body Weight Regulation (J7) Epigenetics
Place of Presentation
Santa Fe
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
