Generation of pancreatic beta cell from human pluripotent stem cell by use of the purification with flow cytometry
| Project/Area Number |
23791025
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Kyoto University |
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Principal Investigator |
TOYODA Taro 京都大学, iPS 細胞研究所, 特定拠点助教 (60593530)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 再生医学 / エネルギー / 糖質代謝異常 / PDX1 / 膵臓β細胞 / インスリン / 糖尿病 / iPS 細胞 / 膵臓 / β細胞 / iPS細胞 |
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| Research Abstract |
Cell therapy using pancreatic ・cells generated from human pluripotent stem cells (hPSCs) is one of the possible solutionsof diabetes problems. Since the generation of target cell types from PSCs with high purity is currently difficult, it is necessary to isolate differentiated pancreatic cells for accurate analysis. In this study, we generated a human iPS cell line in which the fluorescent protein reporter gene is expressed under the control of the promoter of PDX1 gene, a key transcriptional factor for pancreatic development and function. This cell line is useful to analyze pancreatic cells derived from hPSCs
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Report
(3 results)
Research Products
(5 results)
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[Presentation] 病態解析に向けたヒト iPS/ES 細胞から膵外分泌細胞への高効率分化誘導法の開発2012
Author(s)
舩戸道徳, 豊田太郎, 近藤恭士, 細川吉弥,須藤智美, 沖田圭介, 浅香勲, 上杉志成, 加藤善一郎, 太田章, 山中伸弥, 近藤直実, 長船健二
Organizer
第12回日本再生医療学会
Place of Presentation
横浜
Related Report
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