Function of mitochondrial SIRT5 protein and metabolic interactionbetween liver and skeletal muscle

• Project/Area Number: 23791026
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: Kyoto University
• Principal Investigator: NAKAMURA Yasuhiko 京都大学, 医学研究科, 助教 (10375256)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ミトコンドリア / 細胞 / 老化 / 肝臓 / マウス / サーチュイン / 核酸代謝

Research Abstract

We identified urate oxidase (UOX) as a target of SIRT5 by using two-dimensional electrophoresis. Acetylation levels of UOX in liver of SIRT5 Tg mice were approximately half of those in wild-type mice, and UOX activity was significantly increased.

Research Products

• [Journal Article] SIRT5 deacetylates and activates urate oxidase in liver mitochondria of mice (2012)
  • Author(s): Yasuhiko Nakamura, Masahito Ogura, Kasane Ogura, Daisuke Tanaka, Nobuya Inagaki
  • Journal Title: FEBS Lett Volume: 586(23) Issue: 23 Pages: 4076-4081
  • DOI: 10.1016/j.febslet.2012.10.009
  • Peer Reviewed
• [Journal Article] Systems Analysis of GLP-1 Receptor Signaling in Pancreatic {beta}-cells (2011)
  • Author(s): Takeda Y, Amano A, Noma A, Nakamura Y, Fujimoto S, Inagaki N
  • Journal Title: Am J Physiol Cell Physiol Volume: 301(4) Issue: 4 Pages: C792-C803
  • DOI: 10.1152/ajpcell.00057.2011
  • Peer Reviewed
• [Journal Article] Ionic mechanisms and Ca2+ dynamics underlying the glucose response of pancreatic βcells: a simulation study (2011)
  • Author(s): Cha CY , Nakamura Y, Himeno Y, Wang J,Fujimoto S, Inagaki N, Earm YE, Noma A
  • Journal Title: J Gen Physiol Volume: 138(1)巻 Issue: 1 Pages: 21-37
  • DOI: 10.1085/jgp.201110611
  • Peer Reviewed
• [Journal Article] Role of mitochondrial phosphate carrier in metabolism-secretion coupling in rat insulinoma cell line INS-1 (2011)
  • Author(s): Nishi Y, Fujimoto S, Sasaki M, Mukai E, Sato H, Sato Y, Tahara Y, Nakamura Y, Inagaki N
  • Journal Title: Biochem J Volume: 435 Issue: 2 Pages: 421-430
  • DOI: 10.1042/bj20101708
  • Peer Reviewed
• [Presentation] マウス肝ミトコンドリアにおける Sirt5 による urate oxidaseの脱アセチル化と活性化 (2012)
  • Author(s): 中村 靖彦、小倉 雅仁、小倉 かさね、田中 大祐、稲垣 暢也
  • Organizer: 第55回日本糖尿病学会
  • Place of Presentation: 横浜
• [Presentation] Nobuya Inagaki SIRT5 deacetylates and activates urate oxidase in mouse liver mitochondria (2012)
  • Author(s): Yasuhiko Nakamura, Masahito Ogura, Kasane Ogura, Daisuke Tanaka
  • Organizer: 9th International Diabetes Federation Western Pacific Region Congress and 4th Scientific Meeting of the Asian Association for the study of Diabetes
  • Place of Presentation: Kyoto
• [Presentation] マウス肝ミトコンドリアにおけるSirt5によるurate oxidaseの脱アセチル化と活性化
  • Author(s): 中村靖彦
  • Organizer: 第55回日本糖尿病学会大会
  • Place of Presentation: 横浜
• [Presentation] SIRT5 deacetylates and activates urate oxidase in mouse liver mitochondria
  • Author(s): Yasuhiko Nakamura
  • Organizer: 9th International Diabetes Federation Western Pacific Region Congress and 4th Scientific Meeting of the Asian Association for the study of Diabetes
  • Place of Presentation: Kyoto