| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Patient-specific iPS cells were generated from human dermal fibroblasts of 8 lipodystrophic patients by retroviruses containing human Oct3/4, Sox2, Klf4 and c-Myc. These patients included 5 patients with congenital generalized lipodystrophy, 1 patient with familial partial lipodystrophy and 2 patients with acquired lipodystrophy. Adipogenic differentiation properties of several patient-specific iPS cell lines were investigated. After in vitro adipogenic differentiation, differentiated human iPS cells were transplanted into the subcutaneous area of nude mice. The study demonstrated that adipocytes derived from human iPS cells can survive and maintain the differentiated properties in vivo for at least 4 weeks after transplantation.
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