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Elucidation of Pathogenesis and Development of a Cell Therapy in Lipodystrophy Using Human iPS Cells

Research Project

Project/Area Number 23791031
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Metabolomics
Research InstitutionKyoto University

Principal Investigator

NOGUCHI Michio  京都大学, 医学研究科, 医員 (00432394)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords脂肪萎縮症 / ヒトiPS細胞 / 疾患特異的iPS細胞 / 細胞移植 / iPS細胞 / 脂肪細胞分化
Research Abstract

Patient-specific iPS cells were generated from human dermal fibroblasts of 8 lipodystrophic patients by retroviruses containing human Oct3/4, Sox2, Klf4 and c-Myc. These patients included 5 patients with congenital generalized lipodystrophy, 1 patient with familial partial lipodystrophy and 2 patients with acquired lipodystrophy. Adipogenic differentiation properties of several patient-specific iPS cell lines were investigated. After in vitro adipogenic differentiation, differentiated human iPS cells were transplanted into the subcutaneous area of nude mice. The study demonstrated that adipocytes derived from human iPS cells can survive and maintain the differentiated properties in vivo for at least 4 weeks after transplantation.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (27 results)

All 2012 2011 Other

All Journal Article (1 results) Presentation (24 results) Book (2 results)

  • [Journal Article] Induced pluripotent stem cells generated from diabetic patients with mitochondrial DNA A3243G mutation.2012

    • Author(s)
      J. Fujikura, K. Nakao, M. Sone, M. Noguchi, E. Mori, M. Naito, D. Taura, M. Harada-Shiba, I. Kishimoto, A. Watanabe, I. Asaka, K. Hosoda, K. Nakao.
    • Journal Title

      Diabetologia

      Volume: 55 Pages: 1689-98

    • Related Report
      2012 Final Research Report
  • [Presentation] In Vitro Differentiation and Transplantation of Human Pluripotent Stem Cell-derived Adipocytes2012

    • Author(s)
      M. Noguchi, 他.
    • Organizer
      Obesity 2012International Symposium for the Study ofObesity
    • Place of Presentation
      京都市
    • Year and Date
      2012-10-14
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト iPS 細胞由来脂肪細胞の分化誘導と細胞移植2012

    • Author(s)
      野口倫生, 他.
    • Organizer
      第33回日本肥満学会
    • Place of Presentation
      京都市
    • Year and Date
      2012-10-12
    • Related Report
      2012 Final Research Report
  • [Presentation] LMNA 遺伝子異常を伴った家族性部分性脂肪萎縮症患者由来iPS 細胞の脂肪細胞分化の検討2012

    • Author(s)
      松原正樹, 野口倫生, 他.
    • Organizer
      第33回日本肥満学会
    • Place of Presentation
      京都市
    • Year and Date
      2012-10-12
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト多能性幹細胞由来前駆脂肪細胞の単離 -細胞表面マーカーの探索-2012

    • Author(s)
      野口倫生, 他.
    • Organizer
      第33回日本肥満学会
    • Place of Presentation
      京都市
    • Year and Date
      2012-10-11
    • Related Report
      2012 Final Research Report
  • [Presentation] Differentiation and survival of adipocyte-like cells derived from human induced pluripotent stem cells and embryonic stem cells2012

    • Author(s)
      M. Noguchi et al.
    • Organizer
      Differentiation and survival of adipocyte-like cells derived from human induced pluripotent stem cells and embryonic stem cells
    • Year and Date
      2012-05-09
    • Related Report
      2012 Final Research Report
  • [Presentation] LMNA 遺伝子異常を伴った家族性部分性脂肪萎縮症患者由来 iPS 細胞の脂肪細胞分化の検討2012

    • Author(s)
      松原正樹, 野口倫生, 他
    • Organizer
      第85回日本内分泌学会学術総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2012-04-21
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト ips 細胞由来の脂肪細胞の脂肪細胞機能解析と細胞移植(プレナリーセッション)2012

    • Author(s)
      野口倫生, 他.
    • Organizer
      第109回日本内科学会
    • Place of Presentation
      京都市
    • Year and Date
      2012-04-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 脂肪萎縮症に対する脂肪組織移植治療に関する検討2012

    • Author(s)
      森栄作, 野口倫生, 他
    • Organizer
      第109回日本内科学会
    • Place of Presentation
      京都市
    • Year and Date
      2012-04-14
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト iPS 細胞由来の脂肪細胞の脂肪細胞機能解析と細胞移植2012

    • Author(s)
      野口倫生, 他.
    • Organizer
      第109回日本内科学会
    • Place of Presentation
      京都市
    • Year and Date
      2012-04-14
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト iPS/ES 細胞由来脂肪細胞の機能解析2011

    • Author(s)
      中根舞子, 野口倫生, 他.
    • Organizer
      第32回日本肥満学会学術集会
    • Place of Presentation
      淡路市
    • Year and Date
      2011-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト iPS 細胞を用いた脂肪細胞治療法の開発2011

    • Author(s)
      野口倫生, その他.
    • Organizer
      第54回日本糖尿病学会年次学術集会
    • Place of Presentation
      札幌市
    • Year and Date
      2011-05-19
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト iPS 細胞を用いた脂肪萎縮症の細胞治療法の開発2011

    • Author(s)
      野口倫生, その他.
    • Organizer
      第84回日本内分泌学会学術総会
    • Place of Presentation
      神戸市
    • Year and Date
      2011-04-23
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒトiPS細胞を用いた脂肪細胞治療法の開発2011

    • Author(s)
      野口倫生, 細田公則, 森栄作, 中根舞子, 藤倉純二, 海老原健, 中尾一和
    • Organizer
      第54回日本糖尿病学会年次学術集会
    • Place of Presentation
      札幌プリンスホテル(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] ヒトiPS/ES細胞由来脂肪細胞の機能解析2011

    • Author(s)
      中根舞子, 野口倫生, 森栄作, 中尾一泰, 松原正樹, 山本祐二, 藤倉純二, 海老原健, 細田公則, 中尾一和
    • Organizer
      第32回日本肥満学会学術集会
    • Place of Presentation
      兵庫県立淡路夢舞台国際会議場(兵庫県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Differentiationand survival of adipocytes derived from human induced pluripotent stem cells and embryonic stem cells2011

    • Author(s)
      M. Noguchi, K. Hosoda, E. Mori, K. Nakao, M. Nakane, D. Taura, M. Sone, T. Kusakabe, Y. Yamamoto, J. Fujikura, K. Takahashi, K. Ebihara, S. Yamanaka, and K. Nakao
    • Organizer
      OBESITY 2011 29th Annual Scientific Meeting
    • Place of Presentation
      Orlando World Center Marriott (Florida, USA)
    • Related Report
      2011 Research-status Report
  • [Presentation] ヒトiPS細胞由来の脂肪細胞の脂肪細胞機能解析と細胞移植

    • Author(s)
      野口倫生、細田公則、田浦大輔、森栄作、松原正樹、曽根正勝、藤倉純二、海老原健、中尾一和
    • Organizer
      第109回日本内科学会
    • Place of Presentation
      みやこめっせ(京都府)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 脂肪萎縮症に対する脂肪組織移植治療に関する検討

    • Author(s)
      森栄作、細田公則、野口倫生、藤倉純二、孫徹、冨田努、内藤雅喜、日下部徹、海老原健、中尾一和
    • Organizer
      第109回日本内科学会
    • Place of Presentation
      みやこめっせ(京都府)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒトips細胞由来の脂肪細胞の脂肪細胞機能解析と細胞移植

    • Author(s)
      野口倫生、細田公則、田浦大輔、森栄作、松原正樹、曽根正勝、藤倉純二、海老原健、中尾一和
    • Organizer
      第109回日本内科学会
    • Place of Presentation
      みやこめっせ(京都府)
    • Related Report
      2012 Annual Research Report
  • [Presentation] LMNA 遺伝子異常を伴った家族性部分性脂肪萎縮症患者由来iPS 細胞の脂肪細胞分化の検討

    • Author(s)
      松原正樹、藤倉純二、森栄作、中尾一泰、野口倫生、細田公則、中尾一和
    • Organizer
      第85回日本内分泌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Differentiation and survival of adipocyte-like cells derived from human induced pluripotent stem cells and embryonic stem cells

    • Author(s)
      M. Noguchi, K. Hosoda, K. Nakao
    • Organizer
      19th European Congress on Obesity (ECO2012)
    • Place of Presentation
      Lyon Convention Centre(France)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト多能性幹細胞由来前駆脂肪細胞の単離 -細胞表面マーカーの探索-

    • Author(s)
      野口倫生、細田公則、森栄作、松原正樹、藤倉純二、海老原健、中尾一和
    • Organizer
      第33回日本肥満学会
    • Place of Presentation
      ホテルグランヴィア京都(京都府)
    • Related Report
      2012 Annual Research Report
  • [Presentation] LMNA遺伝子異常を伴った家族性部分性脂肪萎縮症患者由来iPS 細胞の脂肪細胞分化の検討

    • Author(s)
      松原正樹、野口倫生、日下部徹、藤倉純二、海老原健、細田公則、中尾一和
    • Organizer
      第33回日本肥満学会
    • Place of Presentation
      ホテルグランヴィア京都(京都府)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒトiPS 細胞由来脂肪細胞の分化誘導と細胞移植

    • Author(s)
      野口倫生、細田公則、森栄作、松原正樹、田浦大輔、曽根正勝、藤倉純二、海老原健、中尾一和
    • Organizer
      第33回日本肥満学会
    • Place of Presentation
      ホテルグランヴィア京都(京都府)
    • Related Report
      2012 Annual Research Report
  • [Presentation] In Vitro Differentiation and Transplantation of Human Pluripotent Stem Cell-derived Adipocytes

    • Author(s)
      M. Noguchi
    • Organizer
      Obesity 2012 International Symposium for the Study of Obesity
    • Place of Presentation
      京都大学芝蘭会館(京都府)
    • Related Report
      2012 Annual Research Report
  • [Book] Human Embryonic and Induced Pluripotent Stem Cells:Lineage-Specific Differentiation Protocols2011

    • Author(s)
      M. Noguchi et al.
    • Publisher
      Springer Science+Business Media LLC
    • Related Report
      2012 Final Research Report
  • [Book] Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols2011

    • Author(s)
      Michio Noguchi et al.
    • Publisher
      Springer Science+Business Media, LLC
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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