| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
To comprehensively find molecular targets of autophagy in β cell, we compared protein expressi on profile in islets from pancreatic β-cell specific autophagy deficient mice (Atg7^-cell) and control mice (Atg7^). We identified ERp57 (PDIA3) significantly increased in Atg7^-cellisletsTo investigate the role of ERp57 in autophagy deficient β cells, we generated tetracycline-inducible Atg7 knock-down (Atg7KD) rat insulinoma INS-1 cells. Then, we additively reduced ERp57 in using siRNA in both INS-1 cells to investigate cell viabilities by sub-G1 analysis. The suppression of ERp57 caused to increase sub-G1 population in Atg7KD INS-1 cells. These data suggested that ERp57 might be induced by autophagy deficiency in β cells and ERp57 had protective effects on autophagy deficient β cells against apoptotic cell death.
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