| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
We have identified a long non-coding RNA, which is located at the antisense region ofCTBP1 (Carboxyl teiminal binding protein 1) and then named this transcript‘CTBP1-AS’. CTBP1-AS is induced by androgen treatment and represses CTBP1. Upregulation of CTBP1-AS in prostate cancer promotes hormone-dependent and castration-resistant tumor growth. In addition, we also demonstrated the interaction of CTBP1-AS with one of the epigenetic modifiers which has RNA-binding domains and then subsequent genome-wide regulation of gene expression. Interestingly, cell cycle regulators are significantly enriched in such regulated genes. Taken together, we demonstrated a novel hormone dependent cell cycle regulation mechanism and CTBP1-AS may be a therapeutic targets for hormone refractory prostate cancer.We also identified and analyzed the functions of androgen-regulated miRNAs. We found one of such miRNA promotes prostate cancer growth and is related with the anti-androgen, bicalutamide-resistant cell proliferation. We are going to perform experiments of xenograft model to show the mechanism of development of hormonerefractory prostate cancer.
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