For elucidation of the mechanism of pituitary gland tumorgenesis focused on BMP system and the development of the medicine cure

• Project/Area Number: 23791057
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: Okayama University
• Principal Investigator: MIYOSHI Tomoko 岡山大学, 医療教育統合開発センター, 助教 (40444674)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 下垂体腫瘍 / 骨形成タンパク / クッシング病 / ソマトスタチン誘導体 / 視床下部 / メタスチン / 内分泌学 / 視床下部細胞 / 骨形成たんぱく / 骨形成蛋白 / 薬物治療

Research Abstract

BMP action was known in corticotrope cells as a negative regulator for the expression of an adrenocorticotropin (ACTH) precursor, proopiomelanocortin (POMC). In corticotrope cells, BMP response is upregulated by somatostatin analogs. Thus, somatostatin analogs suppress CRH-induced ACTH production in corticotrope cells.

Research Products

• [Journal Article] GRK-6 mediates FSH action synergistically enhanced by estrogen and the oocyte in rat granulosa cells (2013)
  • Author(s): Miyoshi T, Otsuka F, Shimasaki S
  • Journal Title: Biochem Biophys Res Commun Volume: 434 Pages: 401-406
  • Peer Reviewed
• [Journal Article] GRK-6 mediates FSH action synergistically enhanced by estrogen and the oocyte in rat granulosa cells. (2013)
  • Author(s): Miyoshi T, Otsuka F, Shimasaki S.
  • Journal Title: Biochem Biophys Res Commun Volume: in press
  • Peer Reviewed
• [Journal Article] Regulatory role of kit ligand-c-kit interaction and oocyte Regulatory role of kit ligand-c-kit interaction and oocyte (2012)
  • Author(s): Miyoshi T, Otsuka F, Nakamura E, Inagaki K, Ogura-Ochi K, Tsukamoto N, Takeda M, Makino H
  • Journal Title: Mol Cell Endocrinol Volume: 358 Pages: 18-26
  • Peer Reviewed
• [Journal Article] BMP action in the pituitary: its possible role in modulating somatostatin sensitivity in pituitary tumor cells (2012)
  • Author(s): Otsuka F, Tsukamoto N, Miyoshi T, Iwasaki Y, Makino H
  • Journal Title: Mol Cell Endocrinol Volume: 349 Pages: 105-110
  • Peer Reviewed
• [Journal Article] Interaction between gonadotropin-releasing hormone and bone morphogenetic protein-6 and -7 signaling in LssT2 gonadotrope cells (2012)
  • Author(s): Takeda M, Otsuka F, Takahashi H, Inagaki K, Miyoshi T, Tsukamoto N, Makino H, Lawson MA
  • Journal Title: Mol Cell Endocrinol Volume: 348 Pages: 147-454
  • Peer Reviewed
• [Journal Article] Regulatory role of kit ligand-c-kit interaction and oocyte factors in steroidogenesis by rat granulosa cells. (2012)
  • Author(s): Miyoshi T, Otsuka F, Nakamura E, Inagaki K, Ogura-Ochi K, Tsukamoto N, Takeda M, Makino H.
  • Journal Title: Mol Cell Endocrinol Volume: 358 Pages: 18-26
  • Peer Reviewed
• [Journal Article] An in vivo role of bone morphogenetic protein-6 in aldosterone production by rat adrenal gland. (2012)
  • Author(s): Matsumoto Y, Otsuka F, Inagaki K, Tsukamoto N, Takano-Narazaki M, Miyoshi T, Nakamura E, Ogura-Ochi K, Takeda M, Makino H.
  • Journal Title: J Steroid Biochem Mol Biol. Volume: 132 Pages: 8-14
  • Peer Reviewed
• [Journal Article] Involvement of bone morphogenetic protein activity in somatostatin actions on ovarian steroidogenesis. (2012)
  • Author(s): Nakamura E, Otsuka F, Inagaki K, Tsukamoto N, Ogura-Ochi K, Miyoshi T, Toma K, Takeda M, Makino H.
  • Journal Title: J Steroid Biochem Mol Biol. Volume: 134 Pages: 67-74
  • Peer Reviewed
• [Journal Article] Interaction between gonadotropin-releasing hormone and bone morphogenetic protein-6 and -7 signaling in LβT2 gonadotrope cells. (2012)
  • Author(s): Takeda M, Otsuka F, Takahashi H, Inagaki K, Miyoshi T, Tsukamoto N, Makino H, Lawson MA.
  • Journal Title: Mol Cell Endocrinol Volume: 348 Pages: 147-154
  • Peer Reviewed
• [Journal Article] Peroxisome proliferator-activated receptor activity is involved in the osteoblastic differentiation regulated by bone morphogenetic proteins and tumor necrosis factor-α. (2012)
  • Author(s): Takano M, Otsuka F, Matsumoto Y, Inagaki K, Takeda M, Nakamura E, Tsukamoto N, Miyoshi T, Sada KE, Makino H.
  • Journal Title: Mol Cell Endocrinol. Volume: 348 Pages: 224-32
  • NAID: 120005232493
  • Peer Reviewed
• [Journal Article] BMP action in the pituitary: its possible role in modulating somatostatin sensitivity in pituitary tumor cells. (2012)
  • Author(s): Otsuka F, Tsukamoto N, Miyoshi T, Iwasaki Y, Makino H.
  • Journal Title: Mol Cell Endocrinol. Volume: 349 Pages: 105-110
  • Peer Reviewed
• [Journal Article] Mutual regulation of growth hormone and bone morphogenetic protein system in steroidogenesis by rat granulosa cells. (2012)
  • Author(s): Nakamura E, Otsuka F, Inagaki K, Miyoshi T, Matsumoto Y, Ogura K, Tsukamoto N, Takeda M, Makino H.
  • Journal Title: Endocrinology. Volume: 153 Pages: 469-480
  • Peer Reviewed
• [Journal Article] Enhanced expression of bone morphogenetic protein system in aldosterone-treated mouse kidneys. (2012)
  • Author(s): Suzuki J, Otsuka F, Matsumoto Y, Inagaki K, Miyoshi T, Takeda M, Tsukamoto N, Nakamura E, Ogura K, Makino H.
  • Journal Title: Hypertens Res. Volume: 35 Pages: 312-317
  • NAID: 10030644434
  • Peer Reviewed
• [Journal Article] Bone morphogenetic protein-3b (BMP-3b) inhibits osteoblast differentiation via Smad2/3 pathway by counteracting Smad1/5/8 signaling. (2012)
  • Author(s): Matsumoto Y, Otsuka F, Hino J, Miyoshi T, Takano M, Miyazato M, Makino H, Kangawa K.
  • Journal Title: Mol Cell Endocrinol. Volume: 350 Pages: 78-86
  • Peer Reviewed
• [Journal Article] Functional interaction of bone morphogenetic protein and growth hormone releasing peptide in adrenocorticotropin regulation by corticotrope cells (2011)
  • Author(s): Tsukamoto N, Otsuka F, Miyoshi T, Inagaki K, Nakamura E, Terasaka T, Takeda M, Ogura T, Iwasaki Y, Makino H
  • Journal Title: Mol Cell Endocrinol Volume: 344 Pages: 41-50
  • Peer Reviewed
• [Journal Article] Activities of bone morphogenetic proteins in prolactin regulation by somatostatin analogs in rat pituitary GH3 cells (2011)
  • Author(s): Tsukamoto N, Otsuka F, Miyoshi T, Inagaki K, Nakamura E, Suzuki J, Ogura T, Iwasaki Y, Makino H
  • Journal Title: Mol Cell Endocrinol Volume: 332 Pages: 163-169
  • NAID: 120003497020
  • Peer Reviewed
• [Presentation] 卵母細胞-顆粒膜細胞間のKL/c-kitおよびGRKによる新たなEstrogen調節系 (2012)
  • Author(s): 三好智子・他
  • Organizer: 第17回日本生殖内分泌学会
  • Place of Presentation: 東京
  • Year and Date: 2012-12-08
• [Presentation] コルチコトロープ細胞におけるACTH分泌調節に及ぼすメラトニンとBMP-4 の役割 (2012)
  • Author(s): 塚本尚子・三好智子・他
  • Organizer: 第39回日本神経内分泌学会
  • Place of Presentation: 北九州
• [Presentation] Effects of BMP-6 on Aldosterone Production by Rat Adrenal Gland In Vivo (2012)
  • Author(s): Tomoko Miyoshi, et. Al
  • Organizer: ENDO2012
  • Place of Presentation: Houston(USA)
• [Presentation] Regulatory Role of Melatonin and BMP-4 in Prolactin Secretion by Rat Pituitary Lactotrope Cells (2012)
  • Author(s): Kanako Ogura-Ochi, Tomoko Miyoshi, et. Al
  • Organizer: ENDO2012
  • Place of Presentation: Houston(USA)
• [Presentation] 下垂体コルチコトロープにおけるACTH分泌制御機構:GHRP/CRHとBMP-4 の機能連関 (2012)
  • Author(s): 塚本尚子・三好智子・他
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] KisspeptinによるGnRH発現調節とBMP/Estrogenの関与 (2012)
  • Author(s): 寺坂友博・三好智子・他
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Lactotrope細胞におけるメラトニンによるPRL分泌制御とその機序 (2012)
  • Author(s): 越智可奈子・三好智子・他
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] Effects of BMP-6 on Aldosterone Production by Rat Adrenal Gland In Vivo (2012)
  • Author(s): Miyoshi T Otsuka F Matsumoto Y Inagaki K Tsukamoto N Nakamura E Takano M Ochi K Takeda M Makino H
  • Organizer: THE ENDOCRINE SOCIETY’S 94th ANNUAL MEETING&EXPO
  • Place of Presentation: Houston,Texas, USA
• [Presentation] Estorogen Facilitates Osteoblast Differentiatlon by Upregulating BMP-4 Signaling (2012)
  • Author(s): Otsuka F Matsumoto Y Takano M Miyoshi T Inagaki K Ochi K Makino H
  • Organizer: THE ENDOCRINE SOCIETY’S 94th ANNUAL MEETING&EXPO
  • Place of Presentation: Houston,Texas, USA
• [Presentation] Regulatory Role of Melatonin and BMP-4 in Prolactin Secretion by Rat Pituitary Lactotrope Cells (2012)
  • Author(s): Ochi K Otsuka F Tsukamoto N Miyoshi T Nakamura E Takeda M Inagaki K Ogura T Iwasaki Y Makino H
  • Organizer: THE ENDOCRINE SOCIETY’S 94th ANNUAL MEETING&EXPO
  • Place of Presentation: Houston,Texas, USA
• [Presentation] BMP-3b inhibits osteoblast differentiation via Smad2/3 pathway by counteracting Smad 1/5/8 signaling (2012)
  • Author(s): Otsuka F Hino J Matsumoto Y Miyoshi T Takano M Miyazato M Makino H Kangawa K
  • Organizer: 15thINTERNATIONAL AND 14th EUROREAN CONGRESS OF ENDOCRINOLOGY
  • Place of Presentation: FLORENCE,ITALY
• [Presentation] Kit Iigand-c-kit interaction regulates estradiol production by rat granulose cells via oocyte factors (2012)
  • Author(s): Miyoshi T Otsuka F Nakamura E Inagaki K Makino H
  • Organizer: 15thINTERNATIONAL AND 14th EUROREAN CONGRESS OF ENDOCRINOLOGY
  • Place of Presentation: 名古屋
• [Presentation] 卵細胞間におけるkit ligand/c-kit連関によるステロイド産出調節の検討 (2012)
  • Author(s): 三好智子、大塚文男、中村絵里、稲垣兼一、武田昌也、塚本尚子、越智可奈子、槇野博史
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] 下垂体コルチコトロープにおけるACTH分泌制御機構：GHRP/CRHとBMP-4の機能連関 (2012)
  • Author(s): 塚本尚子、大塚文男、三好智子、稲垣兼一、岩崎泰正、槇野博史
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] GH/IGF-Iによる生殖内分泌機能への影響：ラット卵細胞顆粒膜細胞を用いた検討 (2012)
  • Author(s): 中村絵里、大塚文男、稲垣兼一、三好智子、塚本尚子、越智可奈子、武田昌也、三村由香里、小倉俊郎、槇野博史
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] コルチコトロープ細胞におけるACTH分泌調節に及ぼすメラトニンとBMP-4の役割 (2012)
  • Author(s): 塚本尚子、大塚文男、越智可奈子、中村絵里、武田昌也、三好智子、稲垣兼一、岩崎泰正、槇野博史
  • Organizer: 第39回日本神経内分泌学会
  • Place of Presentation: 北九州
• [Presentation] GHRP-2 によるACTH分泌調節機序の検討とBMP-4 の関与 (2011)
  • Author(s): 塚本尚子・三好智子・他
  • Organizer: 第38回日本神経内分泌学会学術集会
  • Place of Presentation: 東京
• [Presentation] Functional roles of kit ligand-c-kit interaction and oocyte factors in regulation of steroidogenesis by rat granulosa cells (2011)
  • Author(s): Miyoshi T,et. Al
  • Organizer: Functional roles of kit ligand-c-kit interaction and oocyte factors in regulation of steroidogenesis by rat granulosa cells
  • Place of Presentation: Portland
• [Presentation] A Novel Interaction of Bone Morphogenetic Protein and Growth Hormone-Releasing Peptide in Regulating Adrenocorticotropin Production by Corticotrope Cells (2011)
  • Author(s): Naoko Tsukamoto, Tomoko Miyoshi, et. Al
  • Organizer: ENDO 2011: The 93rd Annual Meeting & Expo
  • Place of Presentation: Boston(USA)
• [Presentation] PRL分泌調節におけるBMP-4 の作用とSSTR反応性の変化 (2011)
  • Author(s): 塚本尚子・三好智子・他
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸
• [Presentation] Interaction of Growth Hormone and Bone Morphogenetic Protein System in Steroidogenesis by Rat Granulosa Cells (2011)
  • Author(s): Eri Nakamura, Fumio Otsuka, Kenichi Inagaki, Tomoko Miyoshi, Naoko Tsukamoto, Mariko Takano, Masaya Takeda, Jiro Suzuki, Toshio Ogura, and Hirofumi Makino
  • Organizer: The 93rd Annual Meeting & Expo
  • Place of Presentation: Boston
• [Presentation] PPAR Activity Modulates the Interaction of Bone Morphogenetic Proteins and Tumor Necrosis Factor-{alpha} in Osteoblast Differentiation (2011)
  • Author(s): Mariko Takano, Fumio Otsuka, Yoshinori Matsumoto, Kenichi Inagaki, Masaya Takeda, Eri Nakamura, Naoko Tsukamoto, Tomoko Miyoshi, Ken-ei Sada, and Hirofumi Makino
  • Organizer: The 93rd Annual Meeting & Expo
  • Place of Presentation: Boston
• [Presentation] A Novel Interaction of Bone Morphogenetic Protein and Growth Hormone-Releasing Peptide in Regulating Adrenocorticotropin Production by Corticotrope Cells (2011)
  • Author(s): Naoko Tsukamoto, Fumio Otsuka, Tomoko Miyoshi, Mariko Takano, Kenichi Inagaki, Eri Nakamura, Tomohiro Terasaka, Toshio Ogura, Yasumasa Iwasaki, and Hirofumi Makino
  • Organizer: The 93rd Annual Meeting & Expo
  • Place of Presentation: Boston
• [Presentation] Effects of Fibroblast Growth Factor-8 on Breast Cancer Cell Mitosis by Regulating Bone Morphogenetic Protein and Estrogen Receptor Actions (2011)
  • Author(s): Fumio Otsuka, Hiroko Masuda, Tomoko Miyoshi, Naoko Tsukamoto, Mariko Takano, Kenichi Inagaki, Yoshinori Matsumoto, Hirofumi Makino, and Hiroyoshi Doihara
  • Organizer: The 93rd Annual Meeting & Expo
  • Place of Presentation: Boston
• [Presentation] Functional roles of kit ligand-c-kit interaction and oocyte factors in regulation of steroidogenesis by rat granulosa cells (2011)
  • Author(s): Miyoshi T, Otsuka F, Inagaki K and Makino H
  • Organizer: 44th Annual Meeting - Society for the Study of Reproduction
  • Place of Presentation: Portland
• [Presentation] 卵胞ステロイド分泌における成長ホルモンの影響：顆粒膜細胞におけるBMPとIGF-Iのクロストーク (2011)
  • Author(s): 中村絵里、大塚文男、稲垣兼一、三好智子、塚本尚子、小倉可奈子、槇野博史
  • Organizer: 第16回日本生殖内分泌学会学術集会
  • Place of Presentation: 東京
• [Presentation] GHRP-2によるACTH分泌調節機序の検討とBMP-4の関与 (2011)
  • Author(s): 塚本尚子、大塚文男、小倉可奈子、中村絵里、武田昌也、三好智子、岩崎泰正、槇野博史
  • Organizer: 第38回日本神経内分泌学会学術集会
  • Place of Presentation: 東京
• [Presentation] 成長ホルモンが及ぼす卵巣内分泌機能への影響とその作用メカニズム (2011)
  • Author(s): 中村絵里、大塚文男、稲垣兼一、三好智子、塚本尚子、小倉可奈子、武田昌也、槇野博史
  • Organizer: 第38回日本神経内分泌学会学術集会
  • Place of Presentation: 東京
• [Presentation] A role of BMP-6 on aldosterone production by rat adrenals in vivo
  • Author(s): 大塚文男 松本佳則 稲垣兼一 三好智子 越智可奈子 中村絵里 塚本尚子 武田昌也 槇野博史
  • Organizer: The 5th International Aldosterone Forum in Japan
  • Place of Presentation: Tokyo
• [Presentation] ソマトスタチンアナログの卵巣ステロイド産生への影響と卵胞BMPシステムの関与
  • Author(s): 中村絵里、大塚文男、稲垣兼一、三好智子、塚本尚子、武田昌也、越智可奈子、当真貴志雄、槇野博史
  • Organizer: 第17回日本生殖内分泌学会
  • Place of Presentation: 東京
• [Presentation] 卵母細胞―顆粒膜細胞間のKL/c-kitおよびGRKによる新たなEstrogen調節系
  • Author(s): 三好智子、大塚文男、中村絵里、稲垣兼一、槇野博史
  • Organizer: 第17回日本生殖内分泌学会
  • Place of Presentation: 東京